Matches in SemOpenAlex for { <https://semopenalex.org/work/W3205122988> ?p ?o ?g. }
- W3205122988 abstract "Salmonella enterica serovar Enteritidis (or Salmonella Enteritidis, SE) is one of the oldest members of the genus Salmonella, based on the date of first description and has only gained prominence as a significant bacterial contaminant of food over the last three or four decades. Currently, SE is the most common Salmonella serovar causing foodborne illnesses. Control measures to alleviate human infections require that food isolates be characterized and this was until recently carried out using Pulsed-Field Gel Electrophoresis (PFGE) and phage typing as the main laboratory subtyping tools for use in demonstrating relatedness of isolates recovered from infected humans and the food source. The results provided by these analytical tools were presented with easy-to-understand and comprehensible nomenclature, however, the techniques were inherently poorly discriminatory, which is attributable to the clonality of SE. The tools have now given way to whole genome sequencing which provides a full and comprehensive genetic attributes of an organism and a very attractive and superior tool for defining an isolate and for inferring genetic relatedness among isolates. A comparative phylogenomic analysis of isolates of choice provides both a visual appreciation of relatedness as well as quantifiable estimates of genetic distance. Despite the considerable information provided by whole genome analysis and development of a phylogenetic tree, the approach does not lend itself to generating a useful nomenclature-based description of SE subtypes. To this end, a highly discriminatory, cost-effective, high throughput, validated single nucleotide based genotypic polymerase chain reaction assay (SNP-PCR) was developed focussing on 60 polymorphic loci. The procedure was used to identify 25 circulating clades of SE, the largest number so far described for this organism. The new subtyping test, which exploited whole genome sequencing data, displays the attributes of an ideal subtyping test: high discrimination, low cost, rapid, highly reproducible and epidemiological concordance. The procedure is useful for identifying the subtype designation of an isolate, for defining the population structure of the organism as well as for surveillance and outbreak detection." @default.
- W3205122988 created "2021-10-25" @default.
- W3205122988 creator A5001131987 @default.
- W3205122988 creator A5004372064 @default.
- W3205122988 creator A5021286386 @default.
- W3205122988 creator A5048705470 @default.
- W3205122988 creator A5059285785 @default.
- W3205122988 creator A5059403533 @default.
- W3205122988 creator A5065961980 @default.
- W3205122988 creator A5071420733 @default.
- W3205122988 date "2021-11-10" @default.
- W3205122988 modified "2023-10-03" @default.
- W3205122988 title "Tracking <i>Salmonella</i> Enteritidis in the Genomics Era: Clade Definition Using a SNP-PCR Assay and Implications for Population Structure" @default.
- W3205122988 cites W1425765506 @default.
- W3205122988 cites W1511663195 @default.
- W3205122988 cites W1545018946 @default.
- W3205122988 cites W1881100847 @default.
- W3205122988 cites W1885623304 @default.
- W3205122988 cites W1909050135 @default.
- W3205122988 cites W1941880120 @default.
- W3205122988 cites W1966057120 @default.
- W3205122988 cites W1966615916 @default.
- W3205122988 cites W1966768621 @default.
- W3205122988 cites W1968062083 @default.
- W3205122988 cites W1981821928 @default.
- W3205122988 cites W1984590590 @default.
- W3205122988 cites W1992158011 @default.
- W3205122988 cites W1993865292 @default.
- W3205122988 cites W2001866904 @default.
- W3205122988 cites W2007217762 @default.
- W3205122988 cites W2009555387 @default.
- W3205122988 cites W2010287357 @default.
- W3205122988 cites W2012336968 @default.
- W3205122988 cites W2029929322 @default.
- W3205122988 cites W2032489215 @default.
- W3205122988 cites W2036388473 @default.
- W3205122988 cites W2041148890 @default.
- W3205122988 cites W2055715438 @default.
- W3205122988 cites W2058550226 @default.
- W3205122988 cites W2066827579 @default.
- W3205122988 cites W2089100760 @default.
- W3205122988 cites W2092229850 @default.
- W3205122988 cites W2094740601 @default.
- W3205122988 cites W2096166657 @default.
- W3205122988 cites W2102321404 @default.
- W3205122988 cites W2105537192 @default.
- W3205122988 cites W2112540214 @default.
- W3205122988 cites W2112821341 @default.
- W3205122988 cites W2117091907 @default.
- W3205122988 cites W2118442768 @default.
- W3205122988 cites W2120967220 @default.
- W3205122988 cites W2122387041 @default.
- W3205122988 cites W2128725117 @default.
- W3205122988 cites W2131607849 @default.
- W3205122988 cites W2132295158 @default.
- W3205122988 cites W2136095103 @default.
- W3205122988 cites W2138546143 @default.
- W3205122988 cites W2140299964 @default.
- W3205122988 cites W2142503351 @default.
- W3205122988 cites W2145512499 @default.
- W3205122988 cites W2147476516 @default.
- W3205122988 cites W2154295605 @default.
- W3205122988 cites W2156961803 @default.
- W3205122988 cites W2164278522 @default.
- W3205122988 cites W2164868452 @default.
- W3205122988 cites W2166065412 @default.
- W3205122988 cites W2170339087 @default.
- W3205122988 cites W2170620030 @default.
- W3205122988 cites W2273187858 @default.
- W3205122988 cites W2289904494 @default.
- W3205122988 cites W2327641866 @default.
- W3205122988 cites W2394763400 @default.
- W3205122988 cites W2395194670 @default.
- W3205122988 cites W2396762411 @default.
- W3205122988 cites W2416892151 @default.
- W3205122988 cites W2473847476 @default.
- W3205122988 cites W2513615729 @default.
- W3205122988 cites W2526226643 @default.
- W3205122988 cites W2558514881 @default.
- W3205122988 cites W2564326523 @default.
- W3205122988 cites W2579940954 @default.
- W3205122988 cites W26060601 @default.
- W3205122988 cites W2608487937 @default.
- W3205122988 cites W2730252722 @default.
- W3205122988 cites W2799524357 @default.
- W3205122988 cites W2803720100 @default.
- W3205122988 cites W2899879084 @default.
- W3205122988 cites W2900384045 @default.
- W3205122988 cites W2904597931 @default.
- W3205122988 cites W2950050732 @default.
- W3205122988 cites W2951211878 @default.
- W3205122988 cites W2963310822 @default.
- W3205122988 cites W2975983989 @default.
- W3205122988 cites W2993005136 @default.
- W3205122988 cites W3016846979 @default.
- W3205122988 cites W3035623041 @default.
- W3205122988 cites W3134153430 @default.
- W3205122988 cites W4241674666 @default.
- W3205122988 cites W4243730337 @default.
- W3205122988 cites W4245621004 @default.