Matches in SemOpenAlex for { <https://semopenalex.org/work/W3205223899> ?p ?o ?g. }
- W3205223899 abstract "Abstract The cholesterol-dependent cytolysin perfringolysin O (PFO) is secreted by Clostridium perfringens as a bacterial virulence factor able to form giant ring-shaped pores that perforate and ultimately lyse mammalian cell membranes. To resolve the kinetics of all steps in the assembly pathway, we have used single-molecule fluorescence imaging to follow the dynamics of PFO on dye-loaded liposomes that lead to opening of a pore and release of the encapsulated dye. Formation of a long-lived membrane-bound PFO dimer nucleates the growth of an irreversible oligomer. The growing oligomer can insert into the membrane and open a pore at stoichiometries ranging from tetramers to full rings (∼35-mers), whereby the rate of insertion increases linearly with the number of subunits. Oligomers that insert before the ring is complete continue to grow by monomer addition post insertion. Overall, our observations suggest that PFO membrane insertion is kinetically controlled." @default.
- W3205223899 created "2021-10-25" @default.
- W3205223899 creator A5003513613 @default.
- W3205223899 creator A5008315680 @default.
- W3205223899 creator A5028427338 @default.
- W3205223899 creator A5047199535 @default.
- W3205223899 creator A5053628410 @default.
- W3205223899 creator A5064331067 @default.
- W3205223899 creator A5066343783 @default.
- W3205223899 creator A5076236652 @default.
- W3205223899 date "2021-10-19" @default.
- W3205223899 modified "2023-10-01" @default.
- W3205223899 title "Single-molecule analysis of the entire perfringolysin O pore formation pathway" @default.
- W3205223899 cites W1507553352 @default.
- W3205223899 cites W1863042354 @default.
- W3205223899 cites W1953565486 @default.
- W3205223899 cites W1970026235 @default.
- W3205223899 cites W1990554391 @default.
- W3205223899 cites W2001276190 @default.
- W3205223899 cites W2003331118 @default.
- W3205223899 cites W2007142983 @default.
- W3205223899 cites W2011865356 @default.
- W3205223899 cites W2014079110 @default.
- W3205223899 cites W2018180763 @default.
- W3205223899 cites W2024791084 @default.
- W3205223899 cites W2027492022 @default.
- W3205223899 cites W2028475005 @default.
- W3205223899 cites W2031286310 @default.
- W3205223899 cites W2031294950 @default.
- W3205223899 cites W2032856741 @default.
- W3205223899 cites W2045227909 @default.
- W3205223899 cites W2046462248 @default.
- W3205223899 cites W2057417107 @default.
- W3205223899 cites W2058482910 @default.
- W3205223899 cites W2063024547 @default.
- W3205223899 cites W2081321639 @default.
- W3205223899 cites W2089934202 @default.
- W3205223899 cites W2090821297 @default.
- W3205223899 cites W2105430590 @default.
- W3205223899 cites W2105703571 @default.
- W3205223899 cites W2130222655 @default.
- W3205223899 cites W2130860011 @default.
- W3205223899 cites W2138150368 @default.
- W3205223899 cites W2139089713 @default.
- W3205223899 cites W2160293301 @default.
- W3205223899 cites W2163246095 @default.
- W3205223899 cites W2195753589 @default.
- W3205223899 cites W2245269023 @default.
- W3205223899 cites W2297203981 @default.
- W3205223899 cites W2341531987 @default.
- W3205223899 cites W2499547421 @default.
- W3205223899 cites W2559284316 @default.
- W3205223899 cites W2584282544 @default.
- W3205223899 cites W2596297068 @default.
- W3205223899 cites W2602465944 @default.
- W3205223899 cites W2616524931 @default.
- W3205223899 cites W2807506288 @default.
- W3205223899 cites W2883353719 @default.
- W3205223899 cites W2886160205 @default.
- W3205223899 cites W2886214535 @default.
- W3205223899 cites W2940944653 @default.
- W3205223899 cites W2950224362 @default.
- W3205223899 cites W2951063320 @default.
- W3205223899 cites W2951391808 @default.
- W3205223899 cites W2953440379 @default.
- W3205223899 cites W2963275059 @default.
- W3205223899 cites W3014455984 @default.
- W3205223899 cites W3091308292 @default.
- W3205223899 cites W3128350817 @default.
- W3205223899 cites W3130254153 @default.
- W3205223899 cites W3158914928 @default.
- W3205223899 doi "https://doi.org/10.1101/2021.10.19.464937" @default.
- W3205223899 hasPublicationYear "2021" @default.
- W3205223899 type Work @default.
- W3205223899 sameAs 3205223899 @default.
- W3205223899 citedByCount "3" @default.
- W3205223899 countsByYear W32052238992021 @default.
- W3205223899 countsByYear W32052238992022 @default.
- W3205223899 crossrefType "posted-content" @default.
- W3205223899 hasAuthorship W3205223899A5003513613 @default.
- W3205223899 hasAuthorship W3205223899A5008315680 @default.
- W3205223899 hasAuthorship W3205223899A5028427338 @default.
- W3205223899 hasAuthorship W3205223899A5047199535 @default.
- W3205223899 hasAuthorship W3205223899A5053628410 @default.
- W3205223899 hasAuthorship W3205223899A5064331067 @default.
- W3205223899 hasAuthorship W3205223899A5066343783 @default.
- W3205223899 hasAuthorship W3205223899A5076236652 @default.
- W3205223899 hasBestOaLocation W32052238991 @default.
- W3205223899 hasConcept C104317684 @default.
- W3205223899 hasConcept C121332964 @default.
- W3205223899 hasConcept C12554922 @default.
- W3205223899 hasConcept C148898269 @default.
- W3205223899 hasConcept C166940927 @default.
- W3205223899 hasConcept C178790620 @default.
- W3205223899 hasConcept C185592680 @default.
- W3205223899 hasConcept C188027245 @default.
- W3205223899 hasConcept C2776403692 @default.
- W3205223899 hasConcept C2779546866 @default.
- W3205223899 hasConcept C2780642029 @default.
- W3205223899 hasConcept C41625074 @default.