FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3205380003> ?p ?o ?g. }

• W3205380003 endingPage "1610" @default.
• W3205380003 startingPage "1600" @default.
• W3205380003 abstract "Ebola virus (EBOV) belongs to the Filoviridae family and causes severe illnesses such as hemorrhagic fever with a high mortality rate up to 90%. Now two antibody drugs termed Inmazeb and Ebanga have been approved for treating EBOV infection. However, clinical studies have demonstrated that the mortality rate of the patients who received these two antibody drugs remains above 30%. Therefore, novel therapeutics with better efficacy is still desired. The isolated human IgG1 constant domain 2 (CH2 domain) has been proposed as a scaffold for the development of C-based single domain antibodies (C-sdAbs) as therapeutic candidates against viral infections and other diseases. Here, we screened and identified a novel C-sdAb termed M24 that targets EBOV glycoprotein (GP) from a C-sdAb phage display library. M24 neutralizes the pseudotype EBOV with IC50 of 0.8 nmol/L (12 ng/mL) and has modest neutralizing activity against authentic EBOV. Epitope determination, including molecular docking and site mutation analysis, discloses that M24 binds to the internal fusion loop (IFL) within GP2, a transmembrane subunit of GP. Interestingly, we found that the binding of M24 to GP at pH 5.5 has dramatically decreased compared to the binding at pH 7.5, which may lead to weak efficacy in the neutralization of authentic EBOV. Since no sdAb against EBOV infection has been reported to date, our results not only give a proof of concept that sdAbs could be utilized for the development of potential therapeutic candidates against EBOV infection, but also provide useful information for the discovery and improvement of anti-EBOV agents." @default.
• W3205380003 created "2021-10-25" @default.
• W3205380003 creator A5018569451 @default.
• W3205380003 creator A5041422262 @default.
• W3205380003 creator A5061099924 @default.
• W3205380003 creator A5070812231 @default.
• W3205380003 creator A5084621264 @default.
• W3205380003 creator A5084662151 @default.
• W3205380003 date "2021-10-11" @default.
• W3205380003 modified "2023-09-30" @default.
• W3205380003 title "Identification and Characterization of a Novel Single Domain Antibody Against Ebola Virus" @default.
• W3205380003 cites W1972063245 @default.
• W3205380003 cites W2012833737 @default.
• W3205380003 cites W2042995802 @default.
• W3205380003 cites W2052505196 @default.
• W3205380003 cites W2059976588 @default.
• W3205380003 cites W2062448305 @default.
• W3205380003 cites W2077153526 @default.
• W3205380003 cites W2077661149 @default.
• W3205380003 cites W2087482145 @default.
• W3205380003 cites W2092048050 @default.
• W3205380003 cites W2093797128 @default.
• W3205380003 cites W2094993149 @default.
• W3205380003 cites W2117131474 @default.
• W3205380003 cites W2128406623 @default.
• W3205380003 cites W2128963410 @default.
• W3205380003 cites W2159725619 @default.
• W3205380003 cites W2173672434 @default.
• W3205380003 cites W2190300929 @default.
• W3205380003 cites W2237639303 @default.
• W3205380003 cites W2274022023 @default.
• W3205380003 cites W2288202420 @default.
• W3205380003 cites W2466466163 @default.
• W3205380003 cites W2507513922 @default.
• W3205380003 cites W2544612685 @default.
• W3205380003 cites W2616753020 @default.
• W3205380003 cites W2616831184 @default.
• W3205380003 cites W2792845868 @default.
• W3205380003 cites W2804822363 @default.
• W3205380003 cites W2890513614 @default.
• W3205380003 cites W2896920028 @default.
• W3205380003 cites W2957804804 @default.
• W3205380003 cites W2958312008 @default.
• W3205380003 cites W2991491848 @default.
• W3205380003 cites W2993846589 @default.
• W3205380003 cites W3004859799 @default.
• W3205380003 cites W3021621689 @default.
• W3205380003 cites W3097467831 @default.
• W3205380003 cites W3119456195 @default.
• W3205380003 doi "https://doi.org/10.1007/s12250-021-00454-z" @default.
• W3205380003 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8502631" @default.
• W3205380003 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34632543" @default.
• W3205380003 hasPublicationYear "2021" @default.
• W3205380003 type Work @default.
• W3205380003 sameAs 3205380003 @default.
• W3205380003 citedByCount "4" @default.
• W3205380003 countsByYear W32053800032022 @default.
• W3205380003 countsByYear W32053800032023 @default.
• W3205380003 crossrefType "journal-article" @default.
• W3205380003 hasAuthorship W3205380003A5018569451 @default.
• W3205380003 hasAuthorship W3205380003A5041422262 @default.
• W3205380003 hasAuthorship W3205380003A5061099924 @default.
• W3205380003 hasAuthorship W3205380003A5070812231 @default.
• W3205380003 hasAuthorship W3205380003A5084621264 @default.
• W3205380003 hasAuthorship W3205380003A5084662151 @default.
• W3205380003 hasBestOaLocation W32053800031 @default.
• W3205380003 hasConcept C14086860 @default.
• W3205380003 hasConcept C159047783 @default.
• W3205380003 hasConcept C159654299 @default.
• W3205380003 hasConcept C186268636 @default.
• W3205380003 hasConcept C195616568 @default.
• W3205380003 hasConcept C203014093 @default.
• W3205380003 hasConcept C2522874641 @default.
• W3205380003 hasConcept C2777469322 @default.
• W3205380003 hasConcept C2777820669 @default.
• W3205380003 hasConcept C2909623084 @default.
• W3205380003 hasConcept C86803240 @default.
• W3205380003 hasConceptScore W3205380003C14086860 @default.
• W3205380003 hasConceptScore W3205380003C159047783 @default.
• W3205380003 hasConceptScore W3205380003C159654299 @default.
• W3205380003 hasConceptScore W3205380003C186268636 @default.
• W3205380003 hasConceptScore W3205380003C195616568 @default.
• W3205380003 hasConceptScore W3205380003C203014093 @default.
• W3205380003 hasConceptScore W3205380003C2522874641 @default.
• W3205380003 hasConceptScore W3205380003C2777469322 @default.
• W3205380003 hasConceptScore W3205380003C2777820669 @default.
• W3205380003 hasConceptScore W3205380003C2909623084 @default.
• W3205380003 hasConceptScore W3205380003C86803240 @default.
• W3205380003 hasIssue "6" @default.
• W3205380003 hasLocation W32053800031 @default.
• W3205380003 hasLocation W32053800032 @default.
• W3205380003 hasLocation W32053800033 @default.
• W3205380003 hasOpenAccess W3205380003 @default.
• W3205380003 hasPrimaryLocation W32053800031 @default.
• W3205380003 hasRelatedWork W1567514421 @default.
• W3205380003 hasRelatedWork W2060284918 @default.
• W3205380003 hasRelatedWork W2173672434 @default.
• W3205380003 hasRelatedWork W2772479624 @default.

• next