FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3205472011> ?p ?o ?g. }

• W3205472011 abstract "UBTF is an HMGB-box DNA binding protein and a necessary Pol I/Pol II basal transcription factor. It has been found that UBTF involves in carcinogenesis and progression of a few cancers. Nevertheless, the the biological function and potential molecular mechanism of UBTF in melanoma are still not clear and need to be clarified.UBTF and GIT1 expressions in melanoma specimens and cell lines were examined by quantitative real-time PCR (qRT-PCR) and Western blot. MTT and colony formation assays were used to investigate the effects of UBTF and GIT1 on melanoma cell proliferation. Cell cycle and apoptosis assays were detected by flow cytometry. Tumor formation assay was used to analyze the effect of UBTF on melanoma growth. Bioinformatics predicting, chromatin immunoprecipitation (ChIP)-qRT-PCR and reporter gene assay were fulfilled for verifing GIT1 as UBTF targeting gene.Here we reported that UBTF mRNA and protein expressions were upregulated in primary melanoma specimens and cell lines. UBTF overexpression facilitated melanoma cell proliferation and cell cycle progression and restrained. Silencing UBTF suppressed cell multiplication, cell cycle progression and tumor growth, and promoted apoptosis. UBTF expression was positively related with GIT1 expression in human melanoma tissues. It was verified that UBTF promoted GIT1 transcription in melanoma cells through binding to the promoter region of GIT1. Furthermore, GIT1 overexpression promoted melanoma cell growth and suppressed apoptosis. Knockdown of GIT1 inhibited cell multiplication and induced apoptosis. Overexpression of GIT1 eliminated the effects of silencing UBTF on melanoma cells. Importantly, UBTF activated MEK1/2-ERK1/2 signalling pathways by upregulating GIT1 expression.Our study demonstrates that UBTF promotes melanoma cell proliferation and cell cycle progression by promoting GIT1 transcription, thereby activating MEK1/2-ERK1/2 signalling pathways. The findings indicate that UBTF plays a crucial function in melanoma and may be a potential therapeutic target for the treatment of this disease." @default.
• W3205472011 created "2021-10-25" @default.
• W3205472011 creator A5006150283 @default.
• W3205472011 creator A5015098000 @default.
• W3205472011 creator A5026235098 @default.
• W3205472011 creator A5030189966 @default.
• W3205472011 creator A5032287435 @default.
• W3205472011 creator A5038951834 @default.
• W3205472011 creator A5057209439 @default.
• W3205472011 creator A5072214656 @default.
• W3205472011 creator A5072673242 @default.
• W3205472011 date "2021-10-18" @default.
• W3205472011 modified "2023-10-05" @default.
• W3205472011 title "UBTF facilitates melanoma progression via modulating MEK1/2-ERK1/2 signalling pathways by promoting GIT1 transcription" @default.
• W3205472011 cites W1927177123 @default.
• W3205472011 cites W1963911710 @default.
• W3205472011 cites W1966699869 @default.
• W3205472011 cites W1969194057 @default.
• W3205472011 cites W1970540591 @default.
• W3205472011 cites W1972882837 @default.
• W3205472011 cites W1983140984 @default.
• W3205472011 cites W1986201152 @default.
• W3205472011 cites W1991356247 @default.
• W3205472011 cites W1999446997 @default.
• W3205472011 cites W2023226118 @default.
• W3205472011 cites W2029033880 @default.
• W3205472011 cites W2035595951 @default.
• W3205472011 cites W2057634589 @default.
• W3205472011 cites W2061006256 @default.
• W3205472011 cites W2079854108 @default.
• W3205472011 cites W2080531393 @default.
• W3205472011 cites W2080786558 @default.
• W3205472011 cites W2090710252 @default.
• W3205472011 cites W2095924529 @default.
• W3205472011 cites W2106657280 @default.
• W3205472011 cites W2123355529 @default.
• W3205472011 cites W2148453050 @default.
• W3205472011 cites W2153780234 @default.
• W3205472011 cites W2154041756 @default.
• W3205472011 cites W2170858841 @default.
• W3205472011 cites W2272984102 @default.
• W3205472011 cites W2275812893 @default.
• W3205472011 cites W2294649300 @default.
• W3205472011 cites W2525697724 @default.
• W3205472011 cites W2570618306 @default.
• W3205472011 cites W2734840967 @default.
• W3205472011 cites W2762347490 @default.
• W3205472011 cites W2781525129 @default.
• W3205472011 cites W2911188335 @default.
• W3205472011 cites W3110967103 @default.
• W3205472011 cites W3137524256 @default.
• W3205472011 doi "https://doi.org/10.1186/s12935-021-02237-8" @default.
• W3205472011 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8522148" @default.
• W3205472011 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34663332" @default.
• W3205472011 hasPublicationYear "2021" @default.
• W3205472011 type Work @default.
• W3205472011 sameAs 3205472011 @default.
• W3205472011 citedByCount "6" @default.
• W3205472011 countsByYear W32054720112022 @default.
• W3205472011 countsByYear W32054720112023 @default.
• W3205472011 crossrefType "journal-article" @default.
• W3205472011 hasAuthorship W3205472011A5006150283 @default.
• W3205472011 hasAuthorship W3205472011A5015098000 @default.
• W3205472011 hasAuthorship W3205472011A5026235098 @default.
• W3205472011 hasAuthorship W3205472011A5030189966 @default.
• W3205472011 hasAuthorship W3205472011A5032287435 @default.
• W3205472011 hasAuthorship W3205472011A5038951834 @default.
• W3205472011 hasAuthorship W3205472011A5057209439 @default.
• W3205472011 hasAuthorship W3205472011A5072214656 @default.
• W3205472011 hasAuthorship W3205472011A5072673242 @default.
• W3205472011 hasBestOaLocation W32054720111 @default.
• W3205472011 hasConcept C101762097 @default.
• W3205472011 hasConcept C104317684 @default.
• W3205472011 hasConcept C119056186 @default.
• W3205472011 hasConcept C121608353 @default.
• W3205472011 hasConcept C134320426 @default.
• W3205472011 hasConcept C150194340 @default.
• W3205472011 hasConcept C173396325 @default.
• W3205472011 hasConcept C190283241 @default.
• W3205472011 hasConcept C2777658100 @default.
• W3205472011 hasConcept C29537977 @default.
• W3205472011 hasConcept C502942594 @default.
• W3205472011 hasConcept C54355233 @default.
• W3205472011 hasConcept C55493867 @default.
• W3205472011 hasConcept C555283112 @default.
• W3205472011 hasConcept C62112901 @default.
• W3205472011 hasConcept C86339819 @default.
• W3205472011 hasConcept C86803240 @default.
• W3205472011 hasConceptScore W3205472011C101762097 @default.
• W3205472011 hasConceptScore W3205472011C104317684 @default.
• W3205472011 hasConceptScore W3205472011C119056186 @default.
• W3205472011 hasConceptScore W3205472011C121608353 @default.
• W3205472011 hasConceptScore W3205472011C134320426 @default.
• W3205472011 hasConceptScore W3205472011C150194340 @default.
• W3205472011 hasConceptScore W3205472011C173396325 @default.
• W3205472011 hasConceptScore W3205472011C190283241 @default.
• W3205472011 hasConceptScore W3205472011C2777658100 @default.
• W3205472011 hasConceptScore W3205472011C29537977 @default.
• W3205472011 hasConceptScore W3205472011C502942594 @default.
• W3205472011 hasConceptScore W3205472011C54355233 @default.

• next