FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3205489849> ?p ?o ?g. }

• W3205489849 endingPage "21" @default.
• W3205489849 startingPage "1" @default.
• W3205489849 abstract "Osteoarthritis (OA), a highly prevalent chronic joint disease, involves a complex network of inflammatory mediators that not only triggers pain and cartilage degeneration but also accelerates disease progression. Traditional Chinese medicinal shenjinhuoxue mixture (SHM) shows anti-inflammatory and analgesic effects against OA with remarkable clinical efficacy. This study explored the mechanism underlying anti-OA properties of SHM and evaluated its efficacy and safety via in vivo experiments. Through network pharmacology and published literature, we identified the key active phytochemicals in SHM, including β-sitosterol, oleanolic acid, licochalcone A, quercetin, isorhamnetin, kaempferol, morusin, lupeol, and pinocembrin; the pivotal targets of which are TLR-4 and NF-κB, eliciting anti-OA activity. These phytochemicals can enter the active pockets of TLR-4 and NF-κB with docking <math xmlns=http://www.w3.org/1998/Math/MathML id=M1> <mtext>score</mtext> <mo>≤</mo> <mo>−</mo> <mn>3.86</mn> <mtext> </mtext> <mtext>kcal</mtext> <mo>/</mo> <mtext>mol</mtext> </math> , as shown in molecular docking models. By using surface plasmon resonance assay, licochalcone A and oleanolic acid were found to have good TLR-4-binding affinity. In OA rats, oral SHM at mid and high doses (8.72 g/kg and 26.2 g/kg) over 6 weeks significantly alleviated mechanical and thermal hyperalgesia ( <math xmlns=http://www.w3.org/1998/Math/MathML id=M2> <mi>P</mi> <mo><</mo> <mn>0.0001</mn> </math> ). Accordingly, the expression of inflammatory mediators (TLR-4, interleukin (IL-) 1 receptor-associated kinase 1 (IRAK1), NF-κB-p65, tumor necrosis factor (TNF-) α, IL-6, and IL-1β), receptor activator of the NF-κB ligand (RANKL), and transient receptor potential vanilloid 1 (TRPV1) in the synovial and cartilage tissue of OA rats was significantly decreased ( <math xmlns=http://www.w3.org/1998/Math/MathML id=M3> <mi>P</mi> <mo><</mo> <mn>0.05</mn> </math> ). Moreover, pathological observation illustrated amelioration of cartilage degeneration and joint injury. In chronic toxicity experiment of rats, SHM at 60 mg/kg demonstrated the safety. SHM had an anti-inflammatory effect through a synergistic combination of active phytochemicals to attenuate pain and cartilage degeneration by inhibiting TLR-4 and NF-κB activation. This study provided the experimental foundation for the development of SHM into a more effective dosage form or new drugs for OA treatment." @default.
• W3205489849 created "2021-10-25" @default.
• W3205489849 creator A5025303085 @default.
• W3205489849 creator A5026292397 @default.
• W3205489849 creator A5029675218 @default.
• W3205489849 creator A5029683591 @default.
• W3205489849 creator A5070942166 @default.
• W3205489849 creator A5071900659 @default.
• W3205489849 creator A5078262808 @default.
• W3205489849 creator A5078656976 @default.
• W3205489849 date "2021-10-21" @default.
• W3205489849 modified "2023-09-27" @default.
• W3205489849 title "Shenjinhuoxue Mixture Attenuates Inflammation, Pain, and Cartilage Degeneration by Inhibiting TLR-4 and NF-κB Activation in Rats with Osteoarthritis: A Synergistic Combination of Multitarget Active Phytochemicals" @default.
• W3205489849 cites W1551306717 @default.
• W3205489849 cites W1586814698 @default.
• W3205489849 cites W1903593728 @default.
• W3205489849 cites W1968652079 @default.
• W3205489849 cites W1969307566 @default.
• W3205489849 cites W1980172418 @default.
• W3205489849 cites W1982415074 @default.
• W3205489849 cites W1987028778 @default.
• W3205489849 cites W2006898142 @default.
• W3205489849 cites W2015788414 @default.
• W3205489849 cites W2033304578 @default.
• W3205489849 cites W2037008013 @default.
• W3205489849 cites W2043249607 @default.
• W3205489849 cites W2046925632 @default.
• W3205489849 cites W2090632754 @default.
• W3205489849 cites W2094371980 @default.
• W3205489849 cites W2097972249 @default.
• W3205489849 cites W2105924489 @default.
• W3205489849 cites W2110598751 @default.
• W3205489849 cites W2115781555 @default.
• W3205489849 cites W2128001125 @default.
• W3205489849 cites W2137122368 @default.
• W3205489849 cites W2139649249 @default.
• W3205489849 cites W2144312770 @default.
• W3205489849 cites W2145028653 @default.
• W3205489849 cites W223345270 @default.
• W3205489849 cites W2284606198 @default.
• W3205489849 cites W2369391196 @default.
• W3205489849 cites W2413373620 @default.
• W3205489849 cites W2473633310 @default.
• W3205489849 cites W2507683743 @default.
• W3205489849 cites W2546525825 @default.
• W3205489849 cites W2548637454 @default.
• W3205489849 cites W2562834111 @default.
• W3205489849 cites W2565330593 @default.
• W3205489849 cites W2577634437 @default.
• W3205489849 cites W2680118380 @default.
• W3205489849 cites W2747571590 @default.
• W3205489849 cites W2768837288 @default.
• W3205489849 cites W2770617105 @default.
• W3205489849 cites W2772784997 @default.
• W3205489849 cites W2783254727 @default.
• W3205489849 cites W2789793216 @default.
• W3205489849 cites W2790312388 @default.
• W3205489849 cites W2791498498 @default.
• W3205489849 cites W2795251222 @default.
• W3205489849 cites W2803949946 @default.
• W3205489849 cites W2809987113 @default.
• W3205489849 cites W2884583228 @default.
• W3205489849 cites W2885452066 @default.
• W3205489849 cites W2889947704 @default.
• W3205489849 cites W2891184420 @default.
• W3205489849 cites W2896617527 @default.
• W3205489849 cites W2900850186 @default.
• W3205489849 cites W2901107708 @default.
• W3205489849 cites W2901151076 @default.
• W3205489849 cites W2915379541 @default.
• W3205489849 cites W2921539094 @default.
• W3205489849 cites W2927221756 @default.
• W3205489849 cites W2937956639 @default.
• W3205489849 cites W2941766873 @default.
• W3205489849 cites W2944694507 @default.
• W3205489849 cites W2946419846 @default.
• W3205489849 cites W2946427746 @default.
• W3205489849 cites W2951905154 @default.
• W3205489849 cites W2952300692 @default.
• W3205489849 cites W2964090301 @default.
• W3205489849 cites W2971013613 @default.
• W3205489849 cites W2972536232 @default.
• W3205489849 cites W2973223006 @default.
• W3205489849 cites W2974242780 @default.
• W3205489849 cites W2988641875 @default.
• W3205489849 cites W2991150004 @default.
• W3205489849 cites W2995046159 @default.
• W3205489849 cites W2999748704 @default.
• W3205489849 cites W3005917808 @default.
• W3205489849 cites W3007505839 @default.
• W3205489849 cites W3012124082 @default.
• W3205489849 cites W3013005131 @default.
• W3205489849 cites W3015673050 @default.
• W3205489849 cites W3016236964 @default.
• W3205489849 cites W3020510838 @default.
• W3205489849 cites W3020887667 @default.
• W3205489849 cites W3033788317 @default.
• W3205489849 cites W3034998433 @default.

• next