FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3205499276> ?p ?o ?g. }

• W3205499276 endingPage "9537" @default.
• W3205499276 startingPage "9519" @default.
• W3205499276 abstract "B-cell precursor acute lymphoblastic leukemia (BCP-ALL) with mixed-lineage leukemia gene rearrangement (MLL-r) is a poor-prognosis subtype for which additional therapeutic targets are urgently needed. Currently no multi-omics data set for primary MLL r patient cells exists that integrates transcriptomics, proteomics and glycomics to gain an inclusive picture of theranostic targets. Methods: We have integrated transcriptomics, proteomics and glycomics to i) obtain the first inclusive picture of primary patient BCP-ALL cells and identify molecular signatures that distinguish leukemic from normal precursor B-cells and ii) better understand the benefits and limitations of the applied technologies to deliver deep molecular sequence data across major cellular biopolymers. Results: MLL-r cells feature an extensive remodeling of their glycocalyx, with increased levels of Core 2-type O-glycans and complex N-glycans as well as significant changes in sialylation and fucosylation. Notably, glycosaminoglycan remodeling from chondroitin sulfate to heparan sulfate was observed. A survival screen, to determine if glycan remodeling enzymes are redundant, identified MGAT1 and NGLY1, essential components of the N-glycosylation/degradation pathway, as highly relevant within this in vitro screening. OGT and OGA, unique enzymes that regulate intracellular O-GlcNAcylation, were also indispensable. Transcriptomics and proteomics further identified Fes and GALNT7-mediated glycosylation as possible therapeutic targets. While there is overall good correlation between transcriptomics and proteomics data, we demonstrate that a systematic combined multi-omics approach delivers important diagnostic information that is missed when applying a single omics technology. Conclusions: Apart from confirming well-known MLL-r BCP-ALL glycoprotein markers, our integrated multi-omics workflow discovered previously unidentified diagnostic/therapeutic protein targets." @default.
• W3205499276 created "2021-10-25" @default.
• W3205499276 creator A5007447740 @default.
• W3205499276 creator A5016980011 @default.
• W3205499276 creator A5018317721 @default.
• W3205499276 creator A5040470585 @default.
• W3205499276 creator A5043680660 @default.
• W3205499276 creator A5054829821 @default.
• W3205499276 creator A5055022776 @default.
• W3205499276 creator A5057326412 @default.
• W3205499276 creator A5059491004 @default.
• W3205499276 creator A5065164000 @default.
• W3205499276 creator A5067174207 @default.
• W3205499276 creator A5069729940 @default.
• W3205499276 creator A5072036320 @default.
• W3205499276 creator A5077589394 @default.
• W3205499276 creator A5086226696 @default.
• W3205499276 creator A5086608958 @default.
• W3205499276 date "2021-01-01" @default.
• W3205499276 modified "2023-09-27" @default.
• W3205499276 title "Glycoproteome remodeling in MLL-rearranged B-cell precursor acute lymphoblastic leukemia" @default.
• W3205499276 cites W1182461782 @default.
• W3205499276 cites W1533948571 @default.
• W3205499276 cites W1602405601 @default.
• W3205499276 cites W1869510227 @default.
• W3205499276 cites W1898194260 @default.
• W3205499276 cites W1974572597 @default.
• W3205499276 cites W1974966545 @default.
• W3205499276 cites W1981254698 @default.
• W3205499276 cites W1993052072 @default.
• W3205499276 cites W1993838215 @default.
• W3205499276 cites W1994041743 @default.
• W3205499276 cites W1997678829 @default.
• W3205499276 cites W2005497166 @default.
• W3205499276 cites W2006986579 @default.
• W3205499276 cites W2007058211 @default.
• W3205499276 cites W2007731313 @default.
• W3205499276 cites W2021314764 @default.
• W3205499276 cites W2024191111 @default.
• W3205499276 cites W2031211461 @default.
• W3205499276 cites W2033560619 @default.
• W3205499276 cites W2042520913 @default.
• W3205499276 cites W2058229406 @default.
• W3205499276 cites W2067219650 @default.
• W3205499276 cites W2072865927 @default.
• W3205499276 cites W2072918899 @default.
• W3205499276 cites W2085862908 @default.
• W3205499276 cites W2089871583 @default.
• W3205499276 cites W2092041579 @default.
• W3205499276 cites W2094911240 @default.
• W3205499276 cites W2100122648 @default.
• W3205499276 cites W2105435030 @default.
• W3205499276 cites W2108339680 @default.
• W3205499276 cites W2120988920 @default.
• W3205499276 cites W2134440814 @default.
• W3205499276 cites W2136011221 @default.
• W3205499276 cites W2137548222 @default.
• W3205499276 cites W2138790009 @default.
• W3205499276 cites W2140868727 @default.
• W3205499276 cites W2146473098 @default.
• W3205499276 cites W2153978505 @default.
• W3205499276 cites W2157150216 @default.
• W3205499276 cites W2159825863 @default.
• W3205499276 cites W2162764916 @default.
• W3205499276 cites W2171521392 @default.
• W3205499276 cites W2176545661 @default.
• W3205499276 cites W2190199302 @default.
• W3205499276 cites W2259447954 @default.
• W3205499276 cites W2262824621 @default.
• W3205499276 cites W2277505199 @default.
• W3205499276 cites W2301744235 @default.
• W3205499276 cites W2311800161 @default.
• W3205499276 cites W2318095071 @default.
• W3205499276 cites W2475195266 @default.
• W3205499276 cites W2520574697 @default.
• W3205499276 cites W2522203795 @default.
• W3205499276 cites W2522355464 @default.
• W3205499276 cites W2544360569 @default.
• W3205499276 cites W2561097795 @default.
• W3205499276 cites W2582404943 @default.
• W3205499276 cites W2584033231 @default.
• W3205499276 cites W2618985326 @default.
• W3205499276 cites W2620975679 @default.
• W3205499276 cites W2751182982 @default.
• W3205499276 cites W2757692042 @default.
• W3205499276 cites W2759969772 @default.
• W3205499276 cites W2782677321 @default.
• W3205499276 cites W2792594611 @default.
• W3205499276 cites W2795307994 @default.
• W3205499276 cites W2799688212 @default.
• W3205499276 cites W2803757278 @default.
• W3205499276 cites W2805271550 @default.
• W3205499276 cites W2808548669 @default.
• W3205499276 cites W2809371507 @default.
• W3205499276 cites W2811346354 @default.
• W3205499276 cites W2838250877 @default.
• W3205499276 cites W2887484375 @default.
• W3205499276 cites W2889741335 @default.

• next