FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3205535888> ?p ?o ?g. }

• W3205535888 endingPage "32" @default.
• W3205535888 startingPage "24" @default.
• W3205535888 abstract "Microsomal protein per gram of liver (MPPGL) is an important scaling factor for bottom-up physiology-based pharmacokinetic modeling and simulation, but data in pediatrics are limited. Therefore, MPPGL was determined in 160 liver samples from pediatric (<i>n</i> = 129) and adult (<i>n</i> = 31) donors obtained from four sources: the University of Maryland Brain and Tissue Bank (UMBTB), tissue retrieval services at the University of Minnesota and University of Pittsburgh, and Sekisui-Xenotech. Tissues were homogenized and subjected to differential centrifugation to prepare microsomes, and cytochrome <i>c</i> reductase activities in tissue homogenates and microsomes were used to estimate cytochrome P450 reductase (POR) activity as a marker of microsomal recovery; microsomal POR content was also assessed by quantitative proteomics. MPPGL values varied 5- to 10-fold within various age groups/developmental stages, and tissue source was identified as a contributing factor. Using a “trimmed” dataset comprised of samples ranging from 3 to 18 years of age common to the four sources, POR protein abundance and activity in microsomes and POR activity in homogenates was lower in UMBTB samples (autopsy) compared with other sources (perfused/flash-frozen). Regression analyses revealed that the UMBTB samples were driving an apparent age effect as no effect of age on log-transformed MPPGL values was observed when the UMBTB samples were excluded. We conclude that a mean±SD MPPGL value of 30.4±1.7 mg/g is representative between one month postnatal age and early adulthood. Potential source effects should be considered for studies involving tissue samples from multiple sources with different procurement and processing procedures. <h3>SIGNIFICANCE STATEMENT</h3> Microsomal protein per gram of liver (MPPGL) is an important scaling factor for bottom up PBPK modeling and simulation, but data in pediatrics are limited. Although MPPGL varies 5- to 10-fold at a given developmental stage, a value of 30.4 ± 1.7 mg/g (mean ± SD) is representative between one month postnatal age and early adulthood. However, when tissue samples are obtained from multiple sources, different procurement and processing procedures may influence the results and should be taken into consideration." @default.
• W3205535888 created "2021-10-25" @default.
• W3205535888 creator A5001681968 @default.
• W3205535888 creator A5001874759 @default.
• W3205535888 creator A5005624600 @default.
• W3205535888 creator A5029949592 @default.
• W3205535888 creator A5044043452 @default.
• W3205535888 creator A5084405945 @default.
• W3205535888 date "2021-10-22" @default.
• W3205535888 modified "2023-10-10" @default.
• W3205535888 title "Ontogeny of Scaling Factors for Pediatric Physiology-Based Pharmacokinetic Modeling and Simulation: Microsomal Protein Per Gram of Liver" @default.
• W3205535888 cites W1589451590 @default.
• W3205535888 cites W1899598719 @default.
• W3205535888 cites W2021022873 @default.
• W3205535888 cites W2055498861 @default.
• W3205535888 cites W2107533226 @default.
• W3205535888 cites W2114542242 @default.
• W3205535888 cites W2116418471 @default.
• W3205535888 cites W2120416054 @default.
• W3205535888 cites W2131259606 @default.
• W3205535888 cites W2139500170 @default.
• W3205535888 cites W2161213051 @default.
• W3205535888 cites W2161296790 @default.
• W3205535888 cites W2169424381 @default.
• W3205535888 cites W2187761001 @default.
• W3205535888 cites W2498448054 @default.
• W3205535888 cites W2612063647 @default.
• W3205535888 cites W2750262502 @default.
• W3205535888 cites W2794990556 @default.
• W3205535888 cites W2801051064 @default.
• W3205535888 cites W2893858554 @default.
• W3205535888 cites W2921257051 @default.
• W3205535888 cites W2944683377 @default.
• W3205535888 cites W3044445445 @default.
• W3205535888 cites W3114373673 @default.
• W3205535888 cites W3210411854 @default.
• W3205535888 cites W4256587082 @default.
• W3205535888 doi "https://doi.org/10.1124/dmd.121.000623" @default.
• W3205535888 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34686522" @default.
• W3205535888 hasPublicationYear "2021" @default.
• W3205535888 type Work @default.
• W3205535888 sameAs 3205535888 @default.
• W3205535888 citedByCount "4" @default.
• W3205535888 countsByYear W32055358882022 @default.
• W3205535888 countsByYear W32055358882023 @default.
• W3205535888 crossrefType "journal-article" @default.
• W3205535888 hasAuthorship W3205535888A5001681968 @default.
• W3205535888 hasAuthorship W3205535888A5001874759 @default.
• W3205535888 hasAuthorship W3205535888A5005624600 @default.
• W3205535888 hasAuthorship W3205535888A5029949592 @default.
• W3205535888 hasAuthorship W3205535888A5044043452 @default.
• W3205535888 hasAuthorship W3205535888A5084405945 @default.
• W3205535888 hasBestOaLocation W32055358881 @default.
• W3205535888 hasConcept C134651460 @default.
• W3205535888 hasConcept C16685009 @default.
• W3205535888 hasConcept C181199279 @default.
• W3205535888 hasConcept C42407357 @default.
• W3205535888 hasConcept C55493867 @default.
• W3205535888 hasConcept C71924100 @default.
• W3205535888 hasConcept C86803240 @default.
• W3205535888 hasConcept C87644729 @default.
• W3205535888 hasConceptScore W3205535888C134651460 @default.
• W3205535888 hasConceptScore W3205535888C16685009 @default.
• W3205535888 hasConceptScore W3205535888C181199279 @default.
• W3205535888 hasConceptScore W3205535888C42407357 @default.
• W3205535888 hasConceptScore W3205535888C55493867 @default.
• W3205535888 hasConceptScore W3205535888C71924100 @default.
• W3205535888 hasConceptScore W3205535888C86803240 @default.
• W3205535888 hasConceptScore W3205535888C87644729 @default.
• W3205535888 hasIssue "1" @default.
• W3205535888 hasLocation W32055358881 @default.
• W3205535888 hasLocation W32055358882 @default.
• W3205535888 hasLocation W32055358883 @default.
• W3205535888 hasOpenAccess W3205535888 @default.
• W3205535888 hasPrimaryLocation W32055358881 @default.
• W3205535888 hasRelatedWork W130996601 @default.
• W3205535888 hasRelatedWork W1973983361 @default.
• W3205535888 hasRelatedWork W1974172069 @default.
• W3205535888 hasRelatedWork W2029367523 @default.
• W3205535888 hasRelatedWork W2042554554 @default.
• W3205535888 hasRelatedWork W2051212618 @default.
• W3205535888 hasRelatedWork W2079939027 @default.
• W3205535888 hasRelatedWork W2091729352 @default.
• W3205535888 hasRelatedWork W2408008987 @default.
• W3205535888 hasRelatedWork W2442865744 @default.
• W3205535888 hasVolume "50" @default.
• W3205535888 isParatext "false" @default.
• W3205535888 isRetracted "false" @default.
• W3205535888 magId "3205535888" @default.
• W3205535888 workType "article" @default.