FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3205855801> ?p ?o ?g. }

• W3205855801 endingPage "23" @default.
• W3205855801 startingPage "23" @default.
• W3205855801 abstract "The European oyster Ostrea edulis played a key role in the North Sea by providing several ecosystem functions and services. Today, O. edulis is classified as severely degraded or functionally extinct in Europe. Marine conservation is focusing on biogenic reef restoration, namely the restoration of O. edulis in Natura 2000 sites of the North Sea. The identification of oyster larvae related to natural spatfalls of restored reefs and monitoring of larval drift is a key aspect of marine protected area management. Morphological identification and distinction from other abundant bivalve larvae using microscopy is difficult. Existing molecular biological methods are expensive and bound to stationary laboratory equipment, or are inadequate in the visualization. In this study, we identified nucleic acid lateral flow immunoassay (NALFIA), a well-established tool in human pathogen diagnostics, as an efficient approach for point-of-care (POC) testing in marine monitoring. Based on the genetic sequence of the mitochondrial cytochrome b of O. edulis , forward and reverse primers were developed. The reverse primer was labelled with fluorescent dye FITC, forward primer with biotin. Reaction on the lateral flow stripe could be realized with a single O. edulis larva in direct PCR with multiplex primers in a portable PCR-cycler. The established NALFIA system can distinguish O. edulis larvae from Crassostrea gigas and Mytilus edulis larvae, respectively. This method offers new approaches in POC testing in marine research and monitoring. It gives quick and clear results, is inexpensive, and could be easily adapted to other species of interest." @default.
• W3205855801 created "2021-10-25" @default.
• W3205855801 creator A5021588340 @default.
• W3205855801 creator A5023978866 @default.
• W3205855801 creator A5065741866 @default.
• W3205855801 creator A5082658226 @default.
• W3205855801 date "2021-01-01" @default.
• W3205855801 modified "2023-10-18" @default.
• W3205855801 title "Direct multiplex PCR-NALFIA to inform marine conservation: Use of an innovative diagnostic tool for the detection of Ostrea edulis larvae" @default.
• W3205855801 cites W1546341007 @default.
• W3205855801 cites W1653760104 @default.
• W3205855801 cites W1977901924 @default.
• W3205855801 cites W1981171976 @default.
• W3205855801 cites W1981609196 @default.
• W3205855801 cites W1987176207 @default.
• W3205855801 cites W1987230442 @default.
• W3205855801 cites W1993919077 @default.
• W3205855801 cites W1995956195 @default.
• W3205855801 cites W2001194562 @default.
• W3205855801 cites W2009119978 @default.
• W3205855801 cites W2013469333 @default.
• W3205855801 cites W2019595860 @default.
• W3205855801 cites W2034354296 @default.
• W3205855801 cites W2035792726 @default.
• W3205855801 cites W2058190483 @default.
• W3205855801 cites W2064256352 @default.
• W3205855801 cites W2085607126 @default.
• W3205855801 cites W2087914924 @default.
• W3205855801 cites W2097230364 @default.
• W3205855801 cites W2105211451 @default.
• W3205855801 cites W2105275554 @default.
• W3205855801 cites W2105613624 @default.
• W3205855801 cites W2109701270 @default.
• W3205855801 cites W2112928209 @default.
• W3205855801 cites W2113892777 @default.
• W3205855801 cites W2117240806 @default.
• W3205855801 cites W2124347172 @default.
• W3205855801 cites W2152763806 @default.
• W3205855801 cites W2153350094 @default.
• W3205855801 cites W2162098634 @default.
• W3205855801 cites W2168620459 @default.
• W3205855801 cites W2418070246 @default.
• W3205855801 cites W2514642609 @default.
• W3205855801 cites W2793595581 @default.
• W3205855801 cites W2793661659 @default.
• W3205855801 cites W2809853783 @default.
• W3205855801 cites W2890342156 @default.
• W3205855801 cites W2900668256 @default.
• W3205855801 cites W2910583421 @default.
• W3205855801 cites W2947942827 @default.
• W3205855801 cites W2966983598 @default.
• W3205855801 cites W2996452764 @default.
• W3205855801 cites W3006955471 @default.
• W3205855801 cites W3047091869 @default.
• W3205855801 cites W3133737854 @default.
• W3205855801 cites W3138671839 @default.
• W3205855801 cites W4230655221 @default.
• W3205855801 doi "https://doi.org/10.1051/alr/2021023" @default.
• W3205855801 hasPublicationYear "2021" @default.
• W3205855801 type Work @default.
• W3205855801 sameAs 3205855801 @default.
• W3205855801 citedByCount "0" @default.
• W3205855801 crossrefType "journal-article" @default.
• W3205855801 hasAuthorship W3205855801A5021588340 @default.
• W3205855801 hasAuthorship W3205855801A5023978866 @default.
• W3205855801 hasAuthorship W3205855801A5065741866 @default.
• W3205855801 hasAuthorship W3205855801A5082658226 @default.
• W3205855801 hasBestOaLocation W32058558012 @default.
• W3205855801 hasConcept C104317684 @default.
• W3205855801 hasConcept C158278297 @default.
• W3205855801 hasConcept C2776327930 @default.
• W3205855801 hasConcept C2776364969 @default.
• W3205855801 hasConcept C2776845067 @default.
• W3205855801 hasConcept C2776991149 @default.
• W3205855801 hasConcept C2777750631 @default.
• W3205855801 hasConcept C2779909984 @default.
• W3205855801 hasConcept C2781028344 @default.
• W3205855801 hasConcept C2781188995 @default.
• W3205855801 hasConcept C2909208804 @default.
• W3205855801 hasConcept C49105822 @default.
• W3205855801 hasConcept C505870484 @default.
• W3205855801 hasConcept C54355233 @default.
• W3205855801 hasConcept C60644358 @default.
• W3205855801 hasConcept C86803240 @default.
• W3205855801 hasConcept C90583042 @default.
• W3205855801 hasConcept C90856448 @default.
• W3205855801 hasConceptScore W3205855801C104317684 @default.
• W3205855801 hasConceptScore W3205855801C158278297 @default.
• W3205855801 hasConceptScore W3205855801C2776327930 @default.
• W3205855801 hasConceptScore W3205855801C2776364969 @default.
• W3205855801 hasConceptScore W3205855801C2776845067 @default.
• W3205855801 hasConceptScore W3205855801C2776991149 @default.
• W3205855801 hasConceptScore W3205855801C2777750631 @default.
• W3205855801 hasConceptScore W3205855801C2779909984 @default.
• W3205855801 hasConceptScore W3205855801C2781028344 @default.
• W3205855801 hasConceptScore W3205855801C2781188995 @default.
• W3205855801 hasConceptScore W3205855801C2909208804 @default.
• W3205855801 hasConceptScore W3205855801C49105822 @default.

• next