Matches in SemOpenAlex for { <https://semopenalex.org/work/W3206058908> ?p ?o ?g. }
- W3206058908 abstract "Abstract Actively maintained close appositions, or contact sites, between organelle membranes, enable the efficient transfer of biomolecules between the various cellular compartments. Several such sites have been described together with their tethering machinery. Despite these advances we are still far from a comprehensive understanding of the function and regulation of most contact sites. To systematically characterize the proteome of contact sites and support the discovery of new tethers and functional molecules, we established a high throughput screening approach in Saccharomyces cerevisiae based on co-localization imaging. We imaged split fluorescence reporters for six different contact sites, two of which have never been studied before, on the background of 1165 strains expressing a mCherry-tagged yeast protein that have a cellular punctate distribution (a hallmark of contact sites). By scoring both co-localization events and effects on reporter size and abundance, we discovered over 100 new potential contact site residents and effectors in yeast. Focusing on several of the newly identified residents, we identified one set of hits as previously unrecognized homologs to Vps13 and Atg2. These proteins share their lipid transport domain, thus expanding this family of lipid transporters. Analysis of another candidate, Ypr097w, which we now call Lec1 ( L ipid-droplet E rgosterol C ortex 1), revealed that this previously uncharacterized protein dynamically shifts between lipid droplets and the cell cortex, and plays a role in regulation of ergosterol distribution in the cell." @default.
- W3206058908 created "2021-10-25" @default.
- W3206058908 creator A5012207898 @default.
- W3206058908 creator A5019160578 @default.
- W3206058908 creator A5023177319 @default.
- W3206058908 creator A5027385934 @default.
- W3206058908 creator A5045167388 @default.
- W3206058908 creator A5051642552 @default.
- W3206058908 creator A5053405804 @default.
- W3206058908 creator A5061166792 @default.
- W3206058908 creator A5068495723 @default.
- W3206058908 creator A5070072152 @default.
- W3206058908 creator A5074493569 @default.
- W3206058908 creator A5074873933 @default.
- W3206058908 creator A5075551606 @default.
- W3206058908 creator A5077051030 @default.
- W3206058908 creator A5080554132 @default.
- W3206058908 creator A5086426687 @default.
- W3206058908 creator A5086561505 @default.
- W3206058908 creator A5086005689 @default.
- W3206058908 date "2021-10-19" @default.
- W3206058908 modified "2023-09-26" @default.
- W3206058908 title "Systematic analysis of membrane contact sites in <i>Saccharomyces cerevisiae</i> uncovers modulators of cellular lipid distribution" @default.
- W3206058908 cites W1507851645 @default.
- W3206058908 cites W1547675180 @default.
- W3206058908 cites W164879481 @default.
- W3206058908 cites W1757201082 @default.
- W3206058908 cites W1803102843 @default.
- W3206058908 cites W1898232548 @default.
- W3206058908 cites W1977436924 @default.
- W3206058908 cites W1984667462 @default.
- W3206058908 cites W1984954439 @default.
- W3206058908 cites W1985128605 @default.
- W3206058908 cites W1987640473 @default.
- W3206058908 cites W1989378845 @default.
- W3206058908 cites W1990459021 @default.
- W3206058908 cites W2001796296 @default.
- W3206058908 cites W2006124679 @default.
- W3206058908 cites W2019351415 @default.
- W3206058908 cites W2024969564 @default.
- W3206058908 cites W2037586865 @default.
- W3206058908 cites W204275195 @default.
- W3206058908 cites W2055370880 @default.
- W3206058908 cites W2062197104 @default.
- W3206058908 cites W2070563411 @default.
- W3206058908 cites W2077475973 @default.
- W3206058908 cites W2078818620 @default.
- W3206058908 cites W2093629098 @default.
- W3206058908 cites W2096717747 @default.
- W3206058908 cites W2096751414 @default.
- W3206058908 cites W2098756782 @default.
- W3206058908 cites W2100837269 @default.
- W3206058908 cites W2101876702 @default.
- W3206058908 cites W2103017472 @default.
- W3206058908 cites W2106425485 @default.
- W3206058908 cites W2115875921 @default.
- W3206058908 cites W2132629607 @default.
- W3206058908 cites W2133614954 @default.
- W3206058908 cites W2139472183 @default.
- W3206058908 cites W2139848107 @default.
- W3206058908 cites W2145970367 @default.
- W3206058908 cites W2146503498 @default.
- W3206058908 cites W2150723052 @default.
- W3206058908 cites W2160360032 @default.
- W3206058908 cites W2160973386 @default.
- W3206058908 cites W2161093157 @default.
- W3206058908 cites W2165615730 @default.
- W3206058908 cites W2169805130 @default.
- W3206058908 cites W2174792969 @default.
- W3206058908 cites W2277267665 @default.
- W3206058908 cites W2317531456 @default.
- W3206058908 cites W2411402390 @default.
- W3206058908 cites W2415459469 @default.
- W3206058908 cites W2552607372 @default.
- W3206058908 cites W2589191356 @default.
- W3206058908 cites W2617667835 @default.
- W3206058908 cites W2741043158 @default.
- W3206058908 cites W2767714822 @default.
- W3206058908 cites W2770672566 @default.
- W3206058908 cites W2773880766 @default.
- W3206058908 cites W2777138242 @default.
- W3206058908 cites W2784909400 @default.
- W3206058908 cites W2802226968 @default.
- W3206058908 cites W2802723872 @default.
- W3206058908 cites W2808551081 @default.
- W3206058908 cites W2810266624 @default.
- W3206058908 cites W2884390408 @default.
- W3206058908 cites W2885910759 @default.
- W3206058908 cites W2900044275 @default.
- W3206058908 cites W2908633314 @default.
- W3206058908 cites W2911498721 @default.
- W3206058908 cites W2919477878 @default.
- W3206058908 cites W2923461283 @default.
- W3206058908 cites W2933277191 @default.
- W3206058908 cites W2935116054 @default.
- W3206058908 cites W2950595506 @default.
- W3206058908 cites W2951357004 @default.
- W3206058908 cites W2956011757 @default.
- W3206058908 cites W2967913301 @default.
- W3206058908 cites W2968769148 @default.