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- W3206133601 abstract "Abstract Prime editing is a universal and very promising precise genome editing technology. However, optimization of prime editor (PE) from different aspects remains vital for its use as a routine tool in plant basic research and crop molecular breeding. In this report, we tested MS2-based prime editor (MS2PE). We fused the M-MLV reverse transcriptase (RT) gene variant to the MS2 RNA binding protein gene, MCP , and allowed the MCP-RT fusion gene to co-express with the SpCas9 nickase gene, SpCas9H840A , and various engineered pegRNAs harboring MS2 RNA (MS2pegR). Compared with control PEs, MS2PEs significantly enhanced editing efficiency at four of six targets in rice protoplasts, and achieved 1.2∼10.1-fold increase in editing efficiency at five of six targets in transgenic rice lines. Furthermore, we tested total 22 different MS2pegR scaffolds, 3 RT variants or genes, 2 MCP variants, and various combinations of the Cas9 nickase, RT, and MCP modules. Our results demonstrated an alternative strategy for enhancing prime editing." @default.
- W3206133601 created "2021-10-25" @default.
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- W3206133601 date "2021-10-21" @default.
- W3206133601 modified "2023-10-17" @default.
- W3206133601 title "MS2 RNA aptamer enhances prime editing in rice" @default.
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- W3206133601 doi "https://doi.org/10.1101/2021.10.20.465209" @default.
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