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• W3206135443 abstract "To explore the effect on food allergy of stachyose. The egg allergen ovalbumin (OVA) was used to induce a food allergy model of BALB/c mice, and different doses of stachyose were given in process. Using enzyme-linked immunosorbent assay (ELISA) methods to detect the levels of IgE, IgG1, histamine and cytokines. And flow cytometry was used to analyze TH1/TH2 balance further. Besides, Hematoxylin-eosin (HE) staining was used to observe changes of intestinal morphology. Lastly, Reverse Transcription-Polymerase Chain Reaction (RT-qPCR) and western Blot was conducted to explore the possible mechanism. Compared with OVA group, serum IgE and IgG1 levels in the low-dose (1mg/mouse) group and high-dose (5mg/mouse) group of stachyose were significantly reduced (P < 0.05); the level of plasma histamine was also decreased significantly (P < 0.05) and the body temperature were decreased. In all, allergic symptoms were alleviated after stachyose treatment. Furthermore, TH1/TH2 balance was improved after stachyose treatment. Lastly, the expression of TLR2 and NF-κB were increased significantly (P < 0.05) in both mRNA and protein levels after stachyose treatment. Food allergy was alleviated through improving TH1/TH2 balance by activating TLR2/NF-κB signal by stachyose." @default.
• W3206135443 created "2021-10-25" @default.
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• W3206135443 date "2021-12-01" @default.
• W3206135443 modified "2023-10-18" @default.
• W3206135443 title "Study on alleviation effect of stachyose on food allergy through TLR2/NF-κB signal pathway in a mouse model" @default.
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• W3206135443 doi "https://doi.org/10.1016/j.lfs.2021.120038" @default.
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