FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3206184510> ?p ?o ?g. }

• W3206184510 endingPage "104224" @default.
• W3206184510 startingPage "104224" @default.
• W3206184510 abstract "• Self-quenching fluorogenic element for Salmo salar was designed and optimized. • The assay was highly specific to S. salar . • The assay was capable of detecting 5 pg target DNA. • The assay embraces a high tolerance to impurities. Loop-mediated isothermal amplification (LAMP) has emerged as an important diagnostic tool for fish authenticity purpose. While the sequence-independent detection methods greatly increased the likelihood of detecting false-positive signals. Instead, target-specific detection of LAMP amplicons can be achieved through the use of target-specific probes or modified primers as biorecognition elements. The present work selected Salmo salar as a case study, and developed a novel LAMP assay coupled with the self-quenching approach for accurate species identification. Specifically, the loop primer (LB-6) was identified as the candidate primer to design the self-quenching element. The fluorophore attached to LB-6 is self-quenched in unbound state. After binding to the dumb bell shaped DNA specifically, the FAM fluorophore is de-quenched, resulting in the fluorescence release. With the novel assay, as little as 5 pg of S. salar DNA could be detected. Moreover, the self-quenching assay embraces a high tolerance to impurities and the positive LAMP results can always be obtained, regardless of the DNA extraction methods and sample treatments. While significant difference with the Ct value according to both the extraction methods and sample treatments, highlighted also an improved LAMP amplification using DNA with higher purity." @default.
• W3206184510 created "2021-10-25" @default.
• W3206184510 creator A5006436767 @default.
• W3206184510 creator A5013680127 @default.
• W3206184510 creator A5031654301 @default.
• W3206184510 creator A5037634062 @default.
• W3206184510 creator A5042261970 @default.
• W3206184510 creator A5044250938 @default.
• W3206184510 creator A5066285440 @default.
• W3206184510 date "2022-01-01" @default.
• W3206184510 modified "2023-09-23" @default.
• W3206184510 title "Rapid identification of Atlantic salmon (Salmo salar) based on loop-mediated isothermal amplification (LAMP) using self-quenching fluorogenic approach" @default.
• W3206184510 cites W1979974453 @default.
• W3206184510 cites W2000112516 @default.
• W3206184510 cites W2006824546 @default.
• W3206184510 cites W2022800117 @default.
• W3206184510 cites W2050515639 @default.
• W3206184510 cites W2052803978 @default.
• W3206184510 cites W2087980045 @default.
• W3206184510 cites W2100808809 @default.
• W3206184510 cites W2105275554 @default.
• W3206184510 cites W2107839390 @default.
• W3206184510 cites W2140209894 @default.
• W3206184510 cites W2286788400 @default.
• W3206184510 cites W2306914672 @default.
• W3206184510 cites W2552308889 @default.
• W3206184510 cites W2569168468 @default.
• W3206184510 cites W2588534993 @default.
• W3206184510 cites W2753105152 @default.
• W3206184510 cites W2789506182 @default.
• W3206184510 cites W2794759388 @default.
• W3206184510 cites W2892291572 @default.
• W3206184510 cites W2915949504 @default.
• W3206184510 cites W2931521133 @default.
• W3206184510 cites W2948179651 @default.
• W3206184510 cites W2963853942 @default.
• W3206184510 cites W2989839883 @default.
• W3206184510 cites W3000172394 @default.
• W3206184510 cites W3008335518 @default.
• W3206184510 cites W3016483228 @default.
• W3206184510 cites W3118801901 @default.
• W3206184510 cites W3133366820 @default.
• W3206184510 cites W3150895182 @default.
• W3206184510 cites W3186610926 @default.
• W3206184510 cites W3209268421 @default.
• W3206184510 cites W838939648 @default.
• W3206184510 doi "https://doi.org/10.1016/j.jfca.2021.104224" @default.
• W3206184510 hasPublicationYear "2022" @default.
• W3206184510 type Work @default.
• W3206184510 sameAs 3206184510 @default.
• W3206184510 citedByCount "5" @default.
• W3206184510 countsByYear W32061845102023 @default.
• W3206184510 crossrefType "journal-article" @default.
• W3206184510 hasAuthorship W3206184510A5006436767 @default.
• W3206184510 hasAuthorship W3206184510A5013680127 @default.
• W3206184510 hasAuthorship W3206184510A5031654301 @default.
• W3206184510 hasAuthorship W3206184510A5037634062 @default.
• W3206184510 hasAuthorship W3206184510A5042261970 @default.
• W3206184510 hasAuthorship W3206184510A5044250938 @default.
• W3206184510 hasAuthorship W3206184510A5066285440 @default.
• W3206184510 hasConcept C104317684 @default.
• W3206184510 hasConcept C120665830 @default.
• W3206184510 hasConcept C121332964 @default.
• W3206184510 hasConcept C121745418 @default.
• W3206184510 hasConcept C153911025 @default.
• W3206184510 hasConcept C178790620 @default.
• W3206184510 hasConcept C185592680 @default.
• W3206184510 hasConcept C2777563447 @default.
• W3206184510 hasConcept C2777940460 @default.
• W3206184510 hasConcept C2778617687 @default.
• W3206184510 hasConcept C2909208804 @default.
• W3206184510 hasConcept C43617362 @default.
• W3206184510 hasConcept C49105822 @default.
• W3206184510 hasConcept C505870484 @default.
• W3206184510 hasConcept C552990157 @default.
• W3206184510 hasConcept C55493867 @default.
• W3206184510 hasConcept C60635243 @default.
• W3206184510 hasConcept C78063203 @default.
• W3206184510 hasConcept C8185291 @default.
• W3206184510 hasConcept C86803240 @default.
• W3206184510 hasConcept C91881484 @default.
• W3206184510 hasConceptScore W3206184510C104317684 @default.
• W3206184510 hasConceptScore W3206184510C120665830 @default.
• W3206184510 hasConceptScore W3206184510C121332964 @default.
• W3206184510 hasConceptScore W3206184510C121745418 @default.
• W3206184510 hasConceptScore W3206184510C153911025 @default.
• W3206184510 hasConceptScore W3206184510C178790620 @default.
• W3206184510 hasConceptScore W3206184510C185592680 @default.
• W3206184510 hasConceptScore W3206184510C2777563447 @default.
• W3206184510 hasConceptScore W3206184510C2777940460 @default.
• W3206184510 hasConceptScore W3206184510C2778617687 @default.
• W3206184510 hasConceptScore W3206184510C2909208804 @default.
• W3206184510 hasConceptScore W3206184510C43617362 @default.
• W3206184510 hasConceptScore W3206184510C49105822 @default.
• W3206184510 hasConceptScore W3206184510C505870484 @default.
• W3206184510 hasConceptScore W3206184510C552990157 @default.
• W3206184510 hasConceptScore W3206184510C55493867 @default.
• W3206184510 hasConceptScore W3206184510C60635243 @default.

• next