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- W3206215945 abstract "Several aspects of the biology and morphology of the toxic dinoflagellate Pyrodinium bahamense van compressum (Bohm) Steidinger, Tester and Taylor, have not yet been fully explored due to the limitations of the approaches so far utilized. Confocal Laser Scanning Microscopy (CLSM) coupled with differential staining technique has revealed new information that have not yet been reported. We present the development of a fast and reproducible sample preparation method that allows observation on the morphology of the organism during transformation from the vegetative to the pellicle cyst stages. Results have shown that bacterial endocytes are closely associated, with the chloroplasts in the algal vegetative cells which are thought to have implications on the survival of the organism. During pellicle encystment induced by changes in the laboratory conditions, extrusion of cytoplasmic materials and bacteria were observed. Laser micrographs reveal that the pellicle cyst body could be mainly composed of lipid globules and reduced forms of the chloroplast. Condensed genetic material, a characteristic of dinoflagellate cells, has also been observed in the pellicle cyst for the first time. Unlike in other dinoflagellate species, the pellicle wall of this organism is thin and irregular in shape. Also, we describe a hypothesis of a possible mechanism of bacterial horizontal transfer as a consequence of the development changes during reversal of the pellicle cyst to vegetative state. (author)" @default.
- W3206215945 created "2021-10-25" @default.
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- W3206215945 date "2011-07-01" @default.
- W3206215945 modified "2023-09-27" @default.
- W3206215945 title "Characterization of some developmental changes in Pyrodinium Bahamense Var. Compressum as Revealed by Confocal Laser Scanning Microscopy (CLSM)" @default.
- W3206215945 hasPublicationYear "2011" @default.
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