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- W3206620110 abstract "We describe an approach to study the conformation of individual proteins during single particle tracking (SPT) in living cells. Binder/tag is based on incorporation of a 7-mer peptide (the tag) into a protein where its solvent exposure is controlled by protein conformation. Only upon exposure can the peptide specifically interact with a reporter protein (the binder). Thus, simple fluorescence localization reflects protein conformation. Through direct excitation of bright dyes, the trajectory and conformation of individual proteins can be followed. Simple protein engineering provides highly specific biosensors suitable for SPT and FRET. We describe tagSrc, tagFyn, tagSyk, tagFAK, and an orthogonal binder/tag pair. SPT showed slowly diffusing islands of activated Src within Src clusters and dynamics of activation in adhesions. Quantitative analysis and stochastic modeling revealed in vivo Src kinetics. The simplicity of binder/tag can provide access to diverse proteins." @default.
- W3206620110 created "2021-10-25" @default.
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- W3206620110 date "2021-10-01" @default.
- W3206620110 modified "2023-10-16" @default.
- W3206620110 title "Biosensors based on peptide exposure show single molecule conformations in live cells" @default.
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