FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3206746295> ?p ?o ?g. }

• W3206746295 endingPage "6745" @default.
• W3206746295 startingPage "6735" @default.
• W3206746295 abstract "In the 2013-2014 and 2015-2016 seasons, quadrivalent live attenuated influenza vaccine (LAIV) showed reduced pandemic 2009 H1N1 (A/H1N1pdm09) vaccine effectiveness (VE) in the U.S. Impaired fitness of A/H1N1pdm09 LAIV strains in primary human nasal epithelial cells (hNEC) was subsequently shown to be associated with reduced VE. As defective viral genes (DVG) have been detected in QLAIV, it was hypothesised that these might play a role in reduced A/H1N1pdm09 fitness. By applying RT-PCR based approaches, DVG for PB2, PB1 and PA segments were detected in all influenza A LAIV strains tested. Absolute quantification of PA vRNA as a biomarker, using a novel digital RT-PCR assay (RT-dPCR), showed that DVG were a minority population (between 10.2 and 27.8 % of PA vRNA) in LAIV, irrespective of subtype or VE. Importantly, no difference was observed between the fitter pre-2009 H1N1 A/New Caledonia/20/1999 (A/NC99) and less fit A/H1N1pdm09 A/Bolivia/509/2013 (A/BOL13), containing medians of 16.0 % and 10.2 % PA DVG, respectively. Manipulating propagation conditions and minimising A/BOL13 PA DVG to 5.2 % failed to improve viral replication in hNEC, suggesting DVG were not limiting A/BOL13 fitness. Conversely, DVG were able to reduce A/NC99 replication in hNEC to A/BOL13-like levels, but only by enrichment of PA DVG to 66.5 % of vRNA. Notably, this required LAIV propagation under conditions markedly different to those used for vaccine production. We conclude from these data that abundance of DVG in QLAIV is not associated with variations in influenza A VE and that the reduced fitness of A/BOL13 previously described was not driven by the presence of DVG." @default.
• W3206746295 created "2021-10-25" @default.
• W3206746295 creator A5039823653 @default.
• W3206746295 creator A5053983028 @default.
• W3206746295 creator A5076378771 @default.
• W3206746295 date "2021-11-01" @default.
• W3206746295 modified "2023-09-25" @default.
• W3206746295 title "Presence of defective viral genes in H1N1 live attenuated influenza vaccine strains is not associated with reduced human cell fitness or vaccine effectiveness" @default.
• W3206746295 cites W1966521561 @default.
• W3206746295 cites W1982110386 @default.
• W3206746295 cites W1993047642 @default.
• W3206746295 cites W2011507014 @default.
• W3206746295 cites W2026646453 @default.
• W3206746295 cites W2041315270 @default.
• W3206746295 cites W2071516717 @default.
• W3206746295 cites W2084324583 @default.
• W3206746295 cites W2087246531 @default.
• W3206746295 cites W2088611642 @default.
• W3206746295 cites W2089686585 @default.
• W3206746295 cites W2091527885 @default.
• W3206746295 cites W2102430683 @default.
• W3206746295 cites W2105042928 @default.
• W3206746295 cites W2107277218 @default.
• W3206746295 cites W2113940052 @default.
• W3206746295 cites W2114389380 @default.
• W3206746295 cites W2144576389 @default.
• W3206746295 cites W2508460506 @default.
• W3206746295 cites W2555837754 @default.
• W3206746295 cites W2566587919 @default.
• W3206746295 cites W2613867010 @default.
• W3206746295 cites W2736423956 @default.
• W3206746295 cites W2737255316 @default.
• W3206746295 cites W2757730809 @default.
• W3206746295 cites W2770423877 @default.
• W3206746295 cites W2780882482 @default.
• W3206746295 cites W2884370122 @default.
• W3206746295 cites W2885175320 @default.
• W3206746295 cites W2945572186 @default.
• W3206746295 cites W2953162568 @default.
• W3206746295 cites W2955972630 @default.
• W3206746295 cites W2963600783 @default.
• W3206746295 cites W3000332435 @default.
• W3206746295 cites W3021246181 @default.
• W3206746295 cites W3026973391 @default.
• W3206746295 cites W3039869766 @default.
• W3206746295 cites W3040122627 @default.
• W3206746295 cites W3134246254 @default.
• W3206746295 doi "https://doi.org/10.1016/j.vaccine.2021.10.011" @default.
• W3206746295 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34663504" @default.
• W3206746295 hasPublicationYear "2021" @default.
• W3206746295 type Work @default.
• W3206746295 sameAs 3206746295 @default.
• W3206746295 citedByCount "4" @default.
• W3206746295 countsByYear W32067462952021 @default.
• W3206746295 countsByYear W32067462952022 @default.
• W3206746295 crossrefType "journal-article" @default.
• W3206746295 hasAuthorship W3206746295A5039823653 @default.
• W3206746295 hasAuthorship W3206746295A5053983028 @default.
• W3206746295 hasAuthorship W3206746295A5076378771 @default.
• W3206746295 hasBestOaLocation W32067462951 @default.
• W3206746295 hasConcept C133239900 @default.
• W3206746295 hasConcept C140704245 @default.
• W3206746295 hasConcept C159047783 @default.
• W3206746295 hasConcept C2522874641 @default.
• W3206746295 hasConcept C2777546802 @default.
• W3206746295 hasConcept C2780689484 @default.
• W3206746295 hasConcept C2908647359 @default.
• W3206746295 hasConcept C71924100 @default.
• W3206746295 hasConcept C86803240 @default.
• W3206746295 hasConcept C99454951 @default.
• W3206746295 hasConceptScore W3206746295C133239900 @default.
• W3206746295 hasConceptScore W3206746295C140704245 @default.
• W3206746295 hasConceptScore W3206746295C159047783 @default.
• W3206746295 hasConceptScore W3206746295C2522874641 @default.
• W3206746295 hasConceptScore W3206746295C2777546802 @default.
• W3206746295 hasConceptScore W3206746295C2780689484 @default.
• W3206746295 hasConceptScore W3206746295C2908647359 @default.
• W3206746295 hasConceptScore W3206746295C71924100 @default.
• W3206746295 hasConceptScore W3206746295C86803240 @default.
• W3206746295 hasConceptScore W3206746295C99454951 @default.
• W3206746295 hasIssue "46" @default.
• W3206746295 hasLocation W32067462951 @default.
• W3206746295 hasLocation W32067462952 @default.
• W3206746295 hasOpenAccess W3206746295 @default.
• W3206746295 hasPrimaryLocation W32067462951 @default.
• W3206746295 hasRelatedWork W2008611427 @default.
• W3206746295 hasRelatedWork W2066771567 @default.
• W3206746295 hasRelatedWork W2068741835 @default.
• W3206746295 hasRelatedWork W2069443938 @default.
• W3206746295 hasRelatedWork W2075097717 @default.
• W3206746295 hasRelatedWork W2091428672 @default.
• W3206746295 hasRelatedWork W2137790856 @default.
• W3206746295 hasRelatedWork W2320976250 @default.
• W3206746295 hasRelatedWork W3003494581 @default.
• W3206746295 hasRelatedWork W3137493153 @default.
• W3206746295 hasVolume "39" @default.
• W3206746295 isParatext "false" @default.
• W3206746295 isRetracted "false" @default.

• next