FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3206827503> ?p ?o ?g. }

• W3206827503 endingPage "114635" @default.
• W3206827503 startingPage "114635" @default.
• W3206827503 abstract "Huazhuojiedu decoction, a Chinese herbal preparation, has been proven to be clinically effective in treating precancerous lesions in gastric cancer (PLGC). This formula is optimized from a classic formula called Ganluxiaodu Dan. Although some experiments have shown that Huazhuojiedu decoction is effective against PLGC, the mechanism remains unclear.To investigate the treatment of PLGC with Huazhuojiedu decoction from the perspective of lncRNA in vitro and in vivo.A PLGC rat model was prepared and randomly divided into a Huazhuojiedu decoction group (HG), a vitacoenzyme group (VG), a model group (MG), and a normal group (CG). Each group was given a corresponding concentration of medicine and distilled water for 10 weeks. The pathological changes in the gastric mucosa were observed by hematoxylin-eosin staining (HE). High-throughput sequencing was performed to detect the differentially expressed lncRNAs in the HG, MG, and CG. Quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR) was used to verify differentially expressed lncRNAs, and rat-human homology information was obtained from the University of California, Santa Cruz (UCSC) Genome Database. Human gastric mucosal epithelial cells (GES-1) were used to prepare precancerous lesions of gastric cancer cells (MC). A Huazhuojiedu decoction drug-containing serum was prepared to treat the MC cells. The effects of the Huazhuojiedu decoction and the lncRNA ENST00000517368 (lnc 517368) knockdown or overexpression on PLGC cell proliferation and apoptosis were evaluated in vitro using CCK-8, flow cytometry, and RT-qPCR.The HE results showed that gastric mucosal pathology was significantly improved in the HG. High-throughput sequencing results showed that compared with the CG, 91 lncRNAs upregulated in the MG were restored and downregulated in the HG (P < 0.05), and 115 lncRNAs downregulated in the MG were restored and upregulated in the HG (P < 0.05). The results of RT-qPCR were consistent with the sequencing results. The differentially expressed genomic rat lncRNA ENSRNOT00000079699 is homologous to human lnc 517368. In cell experiments, high expression of lnc 517368 promoted proliferation and reduced apoptosis in PLGC cells, while the Huazhuojiedu decoction reduced the expression of lnc 517368 and improved cell morphology.Huazhuojiedu decoction inhibited cell proliferation and promoted apoptosis in PLGC cells, and its effect may be partially dependent on the downregulation of lnc 517368." @default.
• W3206827503 created "2021-10-25" @default.
• W3206827503 creator A5018567913 @default.
• W3206827503 creator A5032018471 @default.
• W3206827503 creator A5067926709 @default.
• W3206827503 creator A5071500128 @default.
• W3206827503 creator A5081101216 @default.
• W3206827503 date "2022-01-01" @default.
• W3206827503 modified "2023-10-14" @default.
• W3206827503 title "The therapeutic effect of Huazhuojiedu decoction on precancerous lesions in a gastric cancer model via the regulation of lnc 517368" @default.
• W3206827503 cites W1199913550 @default.
• W3206827503 cites W2526812424 @default.
• W3206827503 cites W2587685360 @default.
• W3206827503 cites W2616464132 @default.
• W3206827503 cites W2625600561 @default.
• W3206827503 cites W2745258459 @default.
• W3206827503 cites W2754916720 @default.
• W3206827503 cites W2784570320 @default.
• W3206827503 cites W2789543684 @default.
• W3206827503 cites W2887216406 @default.
• W3206827503 cites W2890711177 @default.
• W3206827503 cites W2905554350 @default.
• W3206827503 cites W2977396288 @default.
• W3206827503 cites W2986993755 @default.
• W3206827503 cites W2998995956 @default.
• W3206827503 cites W3012182935 @default.
• W3206827503 cites W3033976451 @default.
• W3206827503 cites W3049036781 @default.
• W3206827503 cites W3087268029 @default.
• W3206827503 cites W3088200806 @default.
• W3206827503 cites W3092644478 @default.
• W3206827503 cites W3104427662 @default.
• W3206827503 cites W3127938993 @default.
• W3206827503 cites W3157440289 @default.
• W3206827503 cites W3162505504 @default.
• W3206827503 doi "https://doi.org/10.1016/j.jep.2021.114635" @default.
• W3206827503 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34648901" @default.
• W3206827503 hasPublicationYear "2022" @default.
• W3206827503 type Work @default.
• W3206827503 sameAs 3206827503 @default.
• W3206827503 citedByCount "6" @default.
• W3206827503 countsByYear W32068275032022 @default.
• W3206827503 countsByYear W32068275032023 @default.
• W3206827503 crossrefType "journal-article" @default.
• W3206827503 hasAuthorship W3206827503A5018567913 @default.
• W3206827503 hasAuthorship W3206827503A5032018471 @default.
• W3206827503 hasAuthorship W3206827503A5067926709 @default.
• W3206827503 hasAuthorship W3206827503A5071500128 @default.
• W3206827503 hasAuthorship W3206827503A5081101216 @default.
• W3206827503 hasConcept C121608353 @default.
• W3206827503 hasConcept C125473707 @default.
• W3206827503 hasConcept C126322002 @default.
• W3206827503 hasConcept C142724271 @default.
• W3206827503 hasConcept C188947578 @default.
• W3206827503 hasConcept C190283241 @default.
• W3206827503 hasConcept C190409667 @default.
• W3206827503 hasConcept C203014093 @default.
• W3206827503 hasConcept C204787440 @default.
• W3206827503 hasConcept C2779422922 @default.
• W3206827503 hasConcept C2781443798 @default.
• W3206827503 hasConcept C502942594 @default.
• W3206827503 hasConcept C553184892 @default.
• W3206827503 hasConcept C55493867 @default.
• W3206827503 hasConcept C556039675 @default.
• W3206827503 hasConcept C71924100 @default.
• W3206827503 hasConcept C74864618 @default.
• W3206827503 hasConcept C86803240 @default.
• W3206827503 hasConcept C98274493 @default.
• W3206827503 hasConceptScore W3206827503C121608353 @default.
• W3206827503 hasConceptScore W3206827503C125473707 @default.
• W3206827503 hasConceptScore W3206827503C126322002 @default.
• W3206827503 hasConceptScore W3206827503C142724271 @default.
• W3206827503 hasConceptScore W3206827503C188947578 @default.
• W3206827503 hasConceptScore W3206827503C190283241 @default.
• W3206827503 hasConceptScore W3206827503C190409667 @default.
• W3206827503 hasConceptScore W3206827503C203014093 @default.
• W3206827503 hasConceptScore W3206827503C204787440 @default.
• W3206827503 hasConceptScore W3206827503C2779422922 @default.
• W3206827503 hasConceptScore W3206827503C2781443798 @default.
• W3206827503 hasConceptScore W3206827503C502942594 @default.
• W3206827503 hasConceptScore W3206827503C553184892 @default.
• W3206827503 hasConceptScore W3206827503C55493867 @default.
• W3206827503 hasConceptScore W3206827503C556039675 @default.
• W3206827503 hasConceptScore W3206827503C71924100 @default.
• W3206827503 hasConceptScore W3206827503C74864618 @default.
• W3206827503 hasConceptScore W3206827503C86803240 @default.
• W3206827503 hasConceptScore W3206827503C98274493 @default.
• W3206827503 hasFunder F4320322163 @default.
• W3206827503 hasLocation W32068275031 @default.
• W3206827503 hasLocation W32068275032 @default.
• W3206827503 hasOpenAccess W3206827503 @default.
• W3206827503 hasPrimaryLocation W32068275031 @default.
• W3206827503 hasRelatedWork W1613572233 @default.
• W3206827503 hasRelatedWork W2099326649 @default.
• W3206827503 hasRelatedWork W2353976653 @default.
• W3206827503 hasRelatedWork W2365320098 @default.
• W3206827503 hasRelatedWork W2373755029 @default.
• W3206827503 hasRelatedWork W2379687242 @default.

• next