Matches in SemOpenAlex for { <https://semopenalex.org/work/W3207029257> ?p ?o ?g. }
- W3207029257 endingPage "575" @default.
- W3207029257 startingPage "575" @default.
- W3207029257 abstract "Clostridium perfringens is an anaerobic, spore-forming, and pathogenic bacterium that causes intestinal diseases in humans and animals. In these cases, therapeutic intervention is challenging; because the disease progresses much rapidly. This bacterium can produce 5 main toxins (alpha, beta, epsilon, iota, and a type of enterotoxin) among which the epsilon toxin (ETX) is used for bioterrorism. This toxin can be prevented by immunization with specific immunogenic vaccines. In the present research, we aimed at developing a recombinant chitosan-based nano-vaccine against ETX of C. perfringens and evaluate its effects on the antibody titration against epsilon toxin in BALB/c mice as the vaccine model.The etx gene from C. perfringens type D was cloned and expressed in E. coli. After analysis by SDS-PAGE and western blotting, the expressed products were purified, and the obtained proteins were used for immunization in mice as a chitosan nanoparticle containing recombinant, purified ETX, and protein.The results of ELISA showed that IgA antibody serum level increased sufficiently using recombinant protein with nanoparticle as an oral and injectable formulation. IgG antibody titers increased significantly after administrating the recombinant proteins with nanoparticles through both oral delivery and intravenous injection.In conclusion, the recombinant ETX is suggested as a good candidate for vaccine production against diseases caused by ETX of C. perfringens type D." @default.
- W3207029257 created "2021-10-25" @default.
- W3207029257 creator A5009729360 @default.
- W3207029257 creator A5015323468 @default.
- W3207029257 creator A5038085601 @default.
- W3207029257 creator A5066107024 @default.
- W3207029257 creator A5068895763 @default.
- W3207029257 date "2021-01-01" @default.
- W3207029257 modified "2023-09-27" @default.
- W3207029257 title "Design of a chitosan-based nano vaccine against epsilon toxin of Clostridium perfringens type D and evaluation of its immunogenicity in BALB/c mice" @default.
- W3207029257 cites W1847021296 @default.
- W3207029257 cites W1931534490 @default.
- W3207029257 cites W1968924663 @default.
- W3207029257 cites W1992948287 @default.
- W3207029257 cites W1996006831 @default.
- W3207029257 cites W1997280115 @default.
- W3207029257 cites W2008278254 @default.
- W3207029257 cites W2012593448 @default.
- W3207029257 cites W2012623954 @default.
- W3207029257 cites W2026462493 @default.
- W3207029257 cites W2031704987 @default.
- W3207029257 cites W2035978552 @default.
- W3207029257 cites W2049872880 @default.
- W3207029257 cites W2051365194 @default.
- W3207029257 cites W205215842 @default.
- W3207029257 cites W2057929627 @default.
- W3207029257 cites W2060322739 @default.
- W3207029257 cites W2068159204 @default.
- W3207029257 cites W2105024467 @default.
- W3207029257 cites W2117575563 @default.
- W3207029257 cites W2126462892 @default.
- W3207029257 cites W2132239186 @default.
- W3207029257 cites W2140332419 @default.
- W3207029257 cites W2252685669 @default.
- W3207029257 cites W2303160425 @default.
- W3207029257 cites W2304938203 @default.
- W3207029257 cites W2328852367 @default.
- W3207029257 cites W2727938938 @default.
- W3207029257 cites W2884060194 @default.
- W3207029257 cites W2885835166 @default.
- W3207029257 cites W2946654477 @default.
- W3207029257 cites W4231135626 @default.
- W3207029257 doi "https://doi.org/10.4103/1735-5362.327504" @default.
- W3207029257 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8562408" @default.
- W3207029257 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34760006" @default.
- W3207029257 hasPublicationYear "2021" @default.
- W3207029257 type Work @default.
- W3207029257 sameAs 3207029257 @default.
- W3207029257 citedByCount "4" @default.
- W3207029257 countsByYear W32070292572022 @default.
- W3207029257 countsByYear W32070292572023 @default.
- W3207029257 crossrefType "journal-article" @default.
- W3207029257 hasAuthorship W3207029257A5009729360 @default.
- W3207029257 hasAuthorship W3207029257A5015323468 @default.
- W3207029257 hasAuthorship W3207029257A5038085601 @default.
- W3207029257 hasAuthorship W3207029257A5066107024 @default.
- W3207029257 hasAuthorship W3207029257A5068895763 @default.
- W3207029257 hasBestOaLocation W32070292571 @default.
- W3207029257 hasConcept C104317684 @default.
- W3207029257 hasConcept C159654299 @default.
- W3207029257 hasConcept C185592680 @default.
- W3207029257 hasConcept C203014093 @default.
- W3207029257 hasConcept C2777367657 @default.
- W3207029257 hasConcept C2779116991 @default.
- W3207029257 hasConcept C2779732960 @default.
- W3207029257 hasConcept C2780801004 @default.
- W3207029257 hasConcept C2780868878 @default.
- W3207029257 hasConcept C40767141 @default.
- W3207029257 hasConcept C523546767 @default.
- W3207029257 hasConcept C54355233 @default.
- W3207029257 hasConcept C547475151 @default.
- W3207029257 hasConcept C55493867 @default.
- W3207029257 hasConcept C66999527 @default.
- W3207029257 hasConcept C86803240 @default.
- W3207029257 hasConcept C89423630 @default.
- W3207029257 hasConceptScore W3207029257C104317684 @default.
- W3207029257 hasConceptScore W3207029257C159654299 @default.
- W3207029257 hasConceptScore W3207029257C185592680 @default.
- W3207029257 hasConceptScore W3207029257C203014093 @default.
- W3207029257 hasConceptScore W3207029257C2777367657 @default.
- W3207029257 hasConceptScore W3207029257C2779116991 @default.
- W3207029257 hasConceptScore W3207029257C2779732960 @default.
- W3207029257 hasConceptScore W3207029257C2780801004 @default.
- W3207029257 hasConceptScore W3207029257C2780868878 @default.
- W3207029257 hasConceptScore W3207029257C40767141 @default.
- W3207029257 hasConceptScore W3207029257C523546767 @default.
- W3207029257 hasConceptScore W3207029257C54355233 @default.
- W3207029257 hasConceptScore W3207029257C547475151 @default.
- W3207029257 hasConceptScore W3207029257C55493867 @default.
- W3207029257 hasConceptScore W3207029257C66999527 @default.
- W3207029257 hasConceptScore W3207029257C86803240 @default.
- W3207029257 hasConceptScore W3207029257C89423630 @default.
- W3207029257 hasIssue "6" @default.
- W3207029257 hasLocation W32070292571 @default.
- W3207029257 hasLocation W32070292572 @default.
- W3207029257 hasLocation W32070292573 @default.
- W3207029257 hasLocation W32070292574 @default.
- W3207029257 hasLocation W32070292575 @default.