FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3207095552> ?p ?o ?g. }

• W3207095552 endingPage "268" @default.
• W3207095552 startingPage "268" @default.
• W3207095552 abstract "Background: Diagnostic laboratories are currently required to provide routine testing of asymptomatic staff and patients as a part of their clinical screening for SARS-CoV-2 infection. However, these cohorts display very different disease prevalence from symptomatic individuals and testing capacity for asymptomatic screening is often limited. Group testing is frequently proposed as a possible solution to address this; however, proposals neglect the technical and operational feasibility of implementation in a front-line diagnostic laboratory. Methods: Between October and December 2020, as a seven-week proof of concept, we took into account scientific, technical and operational feasibility to design and implement an adaptive pooling strategy in an NHS diagnostic laboratory in London (UK). We assessed the impact of pooling on analytical sensitivity and modelled the impact of prevalence on pooling strategy. We then considered the operational constraints to model the potential gains in capacity and the requirements for additional staff and infrastructure. Finally, we developed a LIMS-agnostic laboratory automation workflow and software solution and tested the technical feasibility of our adaptive pooling workflow. Results: First, we determined the analytical sensitivity of the implemented SARS-CoV-2 assay to be 250 copies/mL. We then determined that, in a setting with limited analyser capacity, the testing capacity could be increased by two-fold with pooling, however, in a setting with limited reagents, this could rise to a five-fold increase. These capacity increases could be realized with modest additional resource and staffing requirements whilst utilizing up to 76% fewer plastic consumables and 90% fewer reagents. Finally, we successfully implemented a plate-based pooling workflow and tested 920 patient samples using the reagents that would usually be required to process just 222 samples. Conclusions: Adaptive pooled testing is a scientifically, technically and operationally feasible solution to increase testing capacity in frontline NHS diagnostic laboratories." @default.
• W3207095552 created "2021-10-25" @default.
• W3207095552 creator A5005168451 @default.
• W3207095552 creator A5032857509 @default.
• W3207095552 creator A5048417845 @default.
• W3207095552 creator A5053720251 @default.
• W3207095552 creator A5053811549 @default.
• W3207095552 creator A5063808747 @default.
• W3207095552 creator A5083556767 @default.
• W3207095552 creator A5084568496 @default.
• W3207095552 date "2021-10-13" @default.
• W3207095552 modified "2023-09-24" @default.
• W3207095552 title "Rapid design and implementation of an adaptive pooling workflow for SARS-CoV-2 testing in an NHS diagnostic laboratory: a proof-of-concept study" @default.
• W3207095552 cites W1975680434 @default.
• W3207095552 cites W2111423513 @default.
• W3207095552 cites W2172272121 @default.
• W3207095552 cites W3019625944 @default.
• W3207095552 cites W3023701338 @default.
• W3207095552 cites W3032461328 @default.
• W3207095552 cites W3040998563 @default.
• W3207095552 cites W3041522676 @default.
• W3207095552 cites W3080541874 @default.
• W3207095552 cites W3080991685 @default.
• W3207095552 cites W3084313321 @default.
• W3207095552 cites W3088968345 @default.
• W3207095552 cites W3098533462 @default.
• W3207095552 cites W3127220793 @default.
• W3207095552 doi "https://doi.org/10.12688/wellcomeopenres.17226.1" @default.
• W3207095552 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8591522" @default.
• W3207095552 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34796279" @default.
• W3207095552 hasPublicationYear "2021" @default.
• W3207095552 type Work @default.
• W3207095552 sameAs 3207095552 @default.
• W3207095552 citedByCount "4" @default.
• W3207095552 countsByYear W32070955522021 @default.
• W3207095552 countsByYear W32070955522022 @default.
• W3207095552 countsByYear W32070955522023 @default.
• W3207095552 crossrefType "journal-article" @default.
• W3207095552 hasAuthorship W3207095552A5005168451 @default.
• W3207095552 hasAuthorship W3207095552A5032857509 @default.
• W3207095552 hasAuthorship W3207095552A5048417845 @default.
• W3207095552 hasAuthorship W3207095552A5053720251 @default.
• W3207095552 hasAuthorship W3207095552A5053811549 @default.
• W3207095552 hasAuthorship W3207095552A5063808747 @default.
• W3207095552 hasAuthorship W3207095552A5083556767 @default.
• W3207095552 hasAuthorship W3207095552A5084568496 @default.
• W3207095552 hasBestOaLocation W32070955521 @default.
• W3207095552 hasConcept C112930515 @default.
• W3207095552 hasConcept C127413603 @default.
• W3207095552 hasConcept C144133560 @default.
• W3207095552 hasConcept C154945302 @default.
• W3207095552 hasConcept C159110408 @default.
• W3207095552 hasConcept C162853370 @default.
• W3207095552 hasConcept C177212765 @default.
• W3207095552 hasConcept C200601418 @default.
• W3207095552 hasConcept C21547014 @default.
• W3207095552 hasConcept C2777512617 @default.
• W3207095552 hasConcept C41008148 @default.
• W3207095552 hasConcept C62126406 @default.
• W3207095552 hasConcept C70437156 @default.
• W3207095552 hasConcept C71924100 @default.
• W3207095552 hasConcept C77088390 @default.
• W3207095552 hasConceptScore W3207095552C112930515 @default.
• W3207095552 hasConceptScore W3207095552C127413603 @default.
• W3207095552 hasConceptScore W3207095552C144133560 @default.
• W3207095552 hasConceptScore W3207095552C154945302 @default.
• W3207095552 hasConceptScore W3207095552C159110408 @default.
• W3207095552 hasConceptScore W3207095552C162853370 @default.
• W3207095552 hasConceptScore W3207095552C177212765 @default.
• W3207095552 hasConceptScore W3207095552C200601418 @default.
• W3207095552 hasConceptScore W3207095552C21547014 @default.
• W3207095552 hasConceptScore W3207095552C2777512617 @default.
• W3207095552 hasConceptScore W3207095552C41008148 @default.
• W3207095552 hasConceptScore W3207095552C62126406 @default.
• W3207095552 hasConceptScore W3207095552C70437156 @default.
• W3207095552 hasConceptScore W3207095552C71924100 @default.
• W3207095552 hasConceptScore W3207095552C77088390 @default.
• W3207095552 hasFunder F4320311904 @default.
• W3207095552 hasLocation W32070955521 @default.
• W3207095552 hasLocation W32070955522 @default.
• W3207095552 hasLocation W32070955523 @default.
• W3207095552 hasLocation W32070955524 @default.
• W3207095552 hasLocation W32070955525 @default.
• W3207095552 hasOpenAccess W3207095552 @default.
• W3207095552 hasPrimaryLocation W32070955521 @default.
• W3207095552 hasRelatedWork W2039643438 @default.
• W3207095552 hasRelatedWork W2081035100 @default.
• W3207095552 hasRelatedWork W2093645433 @default.
• W3207095552 hasRelatedWork W2132677480 @default.
• W3207095552 hasRelatedWork W2497175360 @default.
• W3207095552 hasRelatedWork W2748952813 @default.
• W3207095552 hasRelatedWork W2899084033 @default.
• W3207095552 hasRelatedWork W3015914646 @default.
• W3207095552 hasRelatedWork W3206324740 @default.
• W3207095552 hasRelatedWork W93689519 @default.
• W3207095552 hasVolume "6" @default.
• W3207095552 isParatext "false" @default.
• W3207095552 isRetracted "false" @default.

• next