Matches in SemOpenAlex for { <https://semopenalex.org/work/W3207138493> ?p ?o ?g. }
- W3207138493 endingPage "319" @default.
- W3207138493 startingPage "312" @default.
- W3207138493 abstract "ABSTRACT. Human strongyloidiasis is one of the neglected tropical diseases caused by infection with soil-transmitted helminth Strongyloides stercoralis . Conventional stool examination, a method commonly used for diagnosis of S. stercoralis , has low sensitivity, especially in the case of light infections. Herein, we developed the droplet digital polymerase chain reaction (ddPCR) assay to detect S. stercoralis larvae in stool and compared its performance with real-time PCR and stool examination techniques (formalin ethyl-acetate concentration technique [FECT] and agar plate culture [APC]). The ddPCR results showed 98% sensitivity and 90% specificity, and real-time PCR showed 82% sensitivity and 76.7% specificity when compared with the microscopic methods. Moreover, ddPCR could detect a single S. stercoralis larva in feces, and cross-reactions with other parasites were not observed. In conclusion, a novel ddPCR method exhibited high sensitivity and specificity for detection of S. stercoralis in stool samples. This technique may help to improve diagnosis, particularly in cases with light infection. In addition, ddPCR technique might be useful for screening patients before starting immunosuppressive drug therapy, and follow-up after treatment of strongyloidiasis." @default.
- W3207138493 created "2021-10-25" @default.
- W3207138493 creator A5010758900 @default.
- W3207138493 creator A5011191090 @default.
- W3207138493 creator A5015201498 @default.
- W3207138493 creator A5018593270 @default.
- W3207138493 creator A5027835162 @default.
- W3207138493 creator A5035941607 @default.
- W3207138493 creator A5035990615 @default.
- W3207138493 creator A5052704139 @default.
- W3207138493 creator A5077528945 @default.
- W3207138493 creator A5086105175 @default.
- W3207138493 creator A5090035309 @default.
- W3207138493 date "2022-01-05" @default.
- W3207138493 modified "2023-10-01" @default.
- W3207138493 title "Development and Efficacy of Droplet Digital PCR for Detection of Strongyloides stercoralis in Stool" @default.
- W3207138493 cites W130818221 @default.
- W3207138493 cites W1466498106 @default.
- W3207138493 cites W162064618 @default.
- W3207138493 cites W1831321011 @default.
- W3207138493 cites W1963735961 @default.
- W3207138493 cites W1966976587 @default.
- W3207138493 cites W1985164442 @default.
- W3207138493 cites W1987982436 @default.
- W3207138493 cites W2001232262 @default.
- W3207138493 cites W2001897463 @default.
- W3207138493 cites W2011204207 @default.
- W3207138493 cites W2027618731 @default.
- W3207138493 cites W2042709093 @default.
- W3207138493 cites W2044348407 @default.
- W3207138493 cites W2045529242 @default.
- W3207138493 cites W2059671660 @default.
- W3207138493 cites W2062529028 @default.
- W3207138493 cites W2072162872 @default.
- W3207138493 cites W2077806387 @default.
- W3207138493 cites W2080350743 @default.
- W3207138493 cites W2088179304 @default.
- W3207138493 cites W2119445265 @default.
- W3207138493 cites W2127958472 @default.
- W3207138493 cites W2138211901 @default.
- W3207138493 cites W2142442640 @default.
- W3207138493 cites W2151173819 @default.
- W3207138493 cites W2161267454 @default.
- W3207138493 cites W2178470325 @default.
- W3207138493 cites W2184237256 @default.
- W3207138493 cites W2261059590 @default.
- W3207138493 cites W2340424608 @default.
- W3207138493 cites W2400068031 @default.
- W3207138493 cites W2563105997 @default.
- W3207138493 cites W2580165056 @default.
- W3207138493 cites W2756226760 @default.
- W3207138493 cites W2759003958 @default.
- W3207138493 cites W2787004398 @default.
- W3207138493 cites W2865387857 @default.
- W3207138493 cites W2887395756 @default.
- W3207138493 cites W2897123201 @default.
- W3207138493 cites W2899905176 @default.
- W3207138493 cites W2905706750 @default.
- W3207138493 cites W3040693278 @default.
- W3207138493 cites W3090464397 @default.
- W3207138493 doi "https://doi.org/10.4269/ajtmh.21-0729" @default.
- W3207138493 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34662861" @default.
- W3207138493 hasPublicationYear "2022" @default.
- W3207138493 type Work @default.
- W3207138493 sameAs 3207138493 @default.
- W3207138493 citedByCount "7" @default.
- W3207138493 countsByYear W32071384932022 @default.
- W3207138493 countsByYear W32071384932023 @default.
- W3207138493 crossrefType "journal-article" @default.
- W3207138493 hasAuthorship W3207138493A5010758900 @default.
- W3207138493 hasAuthorship W3207138493A5011191090 @default.
- W3207138493 hasAuthorship W3207138493A5015201498 @default.
- W3207138493 hasAuthorship W3207138493A5018593270 @default.
- W3207138493 hasAuthorship W3207138493A5027835162 @default.
- W3207138493 hasAuthorship W3207138493A5035941607 @default.
- W3207138493 hasAuthorship W3207138493A5035990615 @default.
- W3207138493 hasAuthorship W3207138493A5052704139 @default.
- W3207138493 hasAuthorship W3207138493A5077528945 @default.
- W3207138493 hasAuthorship W3207138493A5086105175 @default.
- W3207138493 hasAuthorship W3207138493A5090035309 @default.
- W3207138493 hasBestOaLocation W32071384932 @default.
- W3207138493 hasConcept C104317684 @default.
- W3207138493 hasConcept C159047783 @default.
- W3207138493 hasConcept C165901193 @default.
- W3207138493 hasConcept C203014093 @default.
- W3207138493 hasConcept C2778013117 @default.
- W3207138493 hasConcept C2779300420 @default.
- W3207138493 hasConcept C2780473561 @default.
- W3207138493 hasConcept C48126324 @default.
- W3207138493 hasConcept C49105822 @default.
- W3207138493 hasConcept C55493867 @default.
- W3207138493 hasConcept C61716771 @default.
- W3207138493 hasConcept C86803240 @default.
- W3207138493 hasConcept C89423630 @default.
- W3207138493 hasConceptScore W3207138493C104317684 @default.
- W3207138493 hasConceptScore W3207138493C159047783 @default.
- W3207138493 hasConceptScore W3207138493C165901193 @default.
- W3207138493 hasConceptScore W3207138493C203014093 @default.