Matches in SemOpenAlex for { <https://semopenalex.org/work/W3207304493> ?p ?o ?g. }
- W3207304493 endingPage "1370" @default.
- W3207304493 startingPage "1362" @default.
- W3207304493 abstract "Decoding the identity of biomolecules from trace samples is a longstanding goal in the field of biotechnology. Advances in DNA analysis have substantially affected clinical practice and basic research, but corresponding developments for proteins face challenges due to their relative complexity and our inability to amplify them. Despite progress in methods such as mass spectrometry and mass cytometry, single-molecule protein identification remains a highly challenging objective. Towards this end, we combine DNA nanotechnology with single-molecule force spectroscopy to create a mechanically reconfigurable DNA nanoswitch caliper capable of measuring multiple coordinates on single biomolecules with atomic resolution. Using optical tweezers, we demonstrate absolute distance measurements with ångström-level precision for both DNA and peptides, and using multiplexed magnetic tweezers, we demonstrate quantification of relative abundance in mixed samples. Measuring distances between DNA-labelled residues, we perform single-molecule fingerprinting of synthetic and natural peptides, and show discrimination, within a heterogeneous population, between different posttranslational modifications. DNA nanoswitch calipers are a powerful and accessible tool for characterizing distances within nanoscale complexes that will enable new applications in fields such as single-molecule proteomics." @default.
- W3207304493 created "2021-10-25" @default.
- W3207304493 creator A5003644127 @default.
- W3207304493 creator A5007394281 @default.
- W3207304493 creator A5015398607 @default.
- W3207304493 creator A5015933400 @default.
- W3207304493 creator A5022216955 @default.
- W3207304493 creator A5024026827 @default.
- W3207304493 creator A5031086956 @default.
- W3207304493 creator A5038959517 @default.
- W3207304493 creator A5059550260 @default.
- W3207304493 creator A5077711088 @default.
- W3207304493 creator A5078113475 @default.
- W3207304493 creator A5079107653 @default.
- W3207304493 creator A5082968389 @default.
- W3207304493 date "2021-10-21" @default.
- W3207304493 modified "2023-10-10" @default.
- W3207304493 title "Single-molecule mechanical fingerprinting with DNA nanoswitch calipers" @default.
- W3207304493 cites W1966587447 @default.
- W3207304493 cites W1977159900 @default.
- W3207304493 cites W1983362126 @default.
- W3207304493 cites W1993114893 @default.
- W3207304493 cites W1998276013 @default.
- W3207304493 cites W1998596396 @default.
- W3207304493 cites W2001583798 @default.
- W3207304493 cites W2003959167 @default.
- W3207304493 cites W2006055675 @default.
- W3207304493 cites W2013286714 @default.
- W3207304493 cites W2023096047 @default.
- W3207304493 cites W2024996523 @default.
- W3207304493 cites W2027538679 @default.
- W3207304493 cites W2036475903 @default.
- W3207304493 cites W2038218701 @default.
- W3207304493 cites W2041625780 @default.
- W3207304493 cites W2045959839 @default.
- W3207304493 cites W2051225819 @default.
- W3207304493 cites W2055301096 @default.
- W3207304493 cites W2058398195 @default.
- W3207304493 cites W2074304165 @default.
- W3207304493 cites W2080365558 @default.
- W3207304493 cites W2088048869 @default.
- W3207304493 cites W2091964131 @default.
- W3207304493 cites W2100762532 @default.
- W3207304493 cites W2106799894 @default.
- W3207304493 cites W2107885325 @default.
- W3207304493 cites W2114169554 @default.
- W3207304493 cites W2123792512 @default.
- W3207304493 cites W2126466006 @default.
- W3207304493 cites W2151187602 @default.
- W3207304493 cites W2154139219 @default.
- W3207304493 cites W2154475799 @default.
- W3207304493 cites W2163295026 @default.
- W3207304493 cites W2165850619 @default.
- W3207304493 cites W2167441750 @default.
- W3207304493 cites W2170426667 @default.
- W3207304493 cites W2177374825 @default.
- W3207304493 cites W2178366039 @default.
- W3207304493 cites W2297313765 @default.
- W3207304493 cites W2502056833 @default.
- W3207304493 cites W2511569778 @default.
- W3207304493 cites W2789966123 @default.
- W3207304493 cites W2889454651 @default.
- W3207304493 cites W2896321622 @default.
- W3207304493 cites W2947049608 @default.
- W3207304493 cites W2952289344 @default.
- W3207304493 cites W2974217008 @default.
- W3207304493 cites W2979459886 @default.
- W3207304493 cites W2982304938 @default.
- W3207304493 cites W2985937559 @default.
- W3207304493 cites W2991077907 @default.
- W3207304493 cites W2995162887 @default.
- W3207304493 cites W3000435127 @default.
- W3207304493 cites W3128572788 @default.
- W3207304493 cites W3167479850 @default.
- W3207304493 doi "https://doi.org/10.1038/s41565-021-00979-0" @default.
- W3207304493 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34675411" @default.
- W3207304493 hasPublicationYear "2021" @default.
- W3207304493 type Work @default.
- W3207304493 sameAs 3207304493 @default.
- W3207304493 citedByCount "20" @default.
- W3207304493 countsByYear W32073044932021 @default.
- W3207304493 countsByYear W32073044932022 @default.
- W3207304493 countsByYear W32073044932023 @default.
- W3207304493 crossrefType "journal-article" @default.
- W3207304493 hasAuthorship W3207304493A5003644127 @default.
- W3207304493 hasAuthorship W3207304493A5007394281 @default.
- W3207304493 hasAuthorship W3207304493A5015398607 @default.
- W3207304493 hasAuthorship W3207304493A5015933400 @default.
- W3207304493 hasAuthorship W3207304493A5022216955 @default.
- W3207304493 hasAuthorship W3207304493A5024026827 @default.
- W3207304493 hasAuthorship W3207304493A5031086956 @default.
- W3207304493 hasAuthorship W3207304493A5038959517 @default.
- W3207304493 hasAuthorship W3207304493A5059550260 @default.
- W3207304493 hasAuthorship W3207304493A5077711088 @default.
- W3207304493 hasAuthorship W3207304493A5078113475 @default.
- W3207304493 hasAuthorship W3207304493A5079107653 @default.
- W3207304493 hasAuthorship W3207304493A5082968389 @default.
- W3207304493 hasBestOaLocation W32073044932 @default.