Matches in SemOpenAlex for { <https://semopenalex.org/work/W3207493443> ?p ?o ?g. }
- W3207493443 abstract "Abstract Targeting proximity labeling enzymes to specific cellular locations is a viable strategy for profiling subcellular proteomes. Here, we generated transgenic mice expressing a mitochondrial matrix-targeted ascorbate peroxidase (MAX-Tg) to analyze tissue-specific matrix proteomes. Desthiobiotin-phenol labeling of muscle tissues from MAX-Tg mice allowed for efficient profiling of mitochondrial-localized proteins in these tissues. Comparative analysis of matrix proteomes from MAX-Tg muscle tissues revealed differential enrichment of mitochondrial proteins related to energy production in between different muscle groups. Reticulon 4 interacting protein 1 (RTN4IP1), also known as Optic Atrophy-10 (OPA10), was highly enriched in the cardiac and soleus muscles and was found to localize to the mitochondrial matrix via a strong mitochondrial targeting sequence at its N-terminus. Protein structure analysis revealed that RTN4IP1 is an NADPH oxidoreductase with structural homology to bacterial quinone oxidoreductase. Enzymatic activity assays, interactome analysis, and metabolite profiling confirmed a function for RTN4IP1 in coenzyme Q (CoQ) biosynthesis. Rtn4ip1 -knockout C2C12 cells had reduced CoQ9 levels, were vulnerable to oxidative stress, and had decreased oxygen consumption rates and ATP production. Collectively, RTN4IP1 is a mitochondrial antioxidant NADPH oxidoreductase supporting oxidative phosphorylation activity in muscle tissue." @default.
- W3207493443 created "2021-10-25" @default.
- W3207493443 creator A5000327490 @default.
- W3207493443 creator A5001441680 @default.
- W3207493443 creator A5008578682 @default.
- W3207493443 creator A5044722794 @default.
- W3207493443 creator A5049147819 @default.
- W3207493443 creator A5049345951 @default.
- W3207493443 creator A5049434005 @default.
- W3207493443 creator A5056367989 @default.
- W3207493443 creator A5065474597 @default.
- W3207493443 creator A5071104452 @default.
- W3207493443 creator A5073934092 @default.
- W3207493443 creator A5076671510 @default.
- W3207493443 creator A5079490800 @default.
- W3207493443 creator A5082258548 @default.
- W3207493443 date "2021-10-14" @default.
- W3207493443 modified "2023-10-11" @default.
- W3207493443 title "In vivo mitochondrial matrix proteome profiling reveals RTN4IP1/OPA10 as an antioxidant NADPH oxidoreductase" @default.
- W3207493443 cites W1498448116 @default.
- W3207493443 cites W1800061482 @default.
- W3207493443 cites W1846180325 @default.
- W3207493443 cites W1923689855 @default.
- W3207493443 cites W1965903580 @default.
- W3207493443 cites W1980496079 @default.
- W3207493443 cites W1981748166 @default.
- W3207493443 cites W1990828270 @default.
- W3207493443 cites W1997183854 @default.
- W3207493443 cites W2005792416 @default.
- W3207493443 cites W2012034410 @default.
- W3207493443 cites W2023949937 @default.
- W3207493443 cites W2034405939 @default.
- W3207493443 cites W2035156848 @default.
- W3207493443 cites W2035898183 @default.
- W3207493443 cites W2036253883 @default.
- W3207493443 cites W2042033604 @default.
- W3207493443 cites W2075471735 @default.
- W3207493443 cites W2081665845 @default.
- W3207493443 cites W2082516237 @default.
- W3207493443 cites W2098157209 @default.
- W3207493443 cites W2101117598 @default.
- W3207493443 cites W2128606426 @default.
- W3207493443 cites W2150068695 @default.
- W3207493443 cites W2154068452 @default.
- W3207493443 cites W2160737695 @default.
- W3207493443 cites W2162862678 @default.
- W3207493443 cites W2167795167 @default.
- W3207493443 cites W2169575504 @default.
- W3207493443 cites W2180760460 @default.
- W3207493443 cites W2220950257 @default.
- W3207493443 cites W2503158277 @default.
- W3207493443 cites W2503851771 @default.
- W3207493443 cites W2523662230 @default.
- W3207493443 cites W2549188196 @default.
- W3207493443 cites W2585958136 @default.
- W3207493443 cites W2601885162 @default.
- W3207493443 cites W2604738787 @default.
- W3207493443 cites W2611090362 @default.
- W3207493443 cites W2754373993 @default.
- W3207493443 cites W2765968230 @default.
- W3207493443 cites W2768451093 @default.
- W3207493443 cites W2773803332 @default.
- W3207493443 cites W2783829661 @default.
- W3207493443 cites W2802800214 @default.
- W3207493443 cites W2810092780 @default.
- W3207493443 cites W2888618670 @default.
- W3207493443 cites W2889921316 @default.
- W3207493443 cites W2899664253 @default.
- W3207493443 cites W2899760200 @default.
- W3207493443 cites W2900569176 @default.
- W3207493443 cites W2907218940 @default.
- W3207493443 cites W2944797582 @default.
- W3207493443 cites W2945112778 @default.
- W3207493443 cites W2946554731 @default.
- W3207493443 cites W2973648545 @default.
- W3207493443 cites W2980472864 @default.
- W3207493443 cites W2981179816 @default.
- W3207493443 cites W2982570926 @default.
- W3207493443 cites W2983713778 @default.
- W3207493443 cites W2996045117 @default.
- W3207493443 cites W3003960907 @default.
- W3207493443 cites W3022144399 @default.
- W3207493443 cites W3024644845 @default.
- W3207493443 cites W3024928082 @default.
- W3207493443 cites W3091843058 @default.
- W3207493443 cites W3106539179 @default.
- W3207493443 cites W3131597949 @default.
- W3207493443 cites W3160119673 @default.
- W3207493443 cites W3177828909 @default.
- W3207493443 doi "https://doi.org/10.1101/2021.10.14.464368" @default.
- W3207493443 hasPublicationYear "2021" @default.
- W3207493443 type Work @default.
- W3207493443 sameAs 3207493443 @default.
- W3207493443 citedByCount "3" @default.
- W3207493443 countsByYear W32074934432022 @default.
- W3207493443 crossrefType "posted-content" @default.
- W3207493443 hasAuthorship W3207493443A5000327490 @default.
- W3207493443 hasAuthorship W3207493443A5001441680 @default.
- W3207493443 hasAuthorship W3207493443A5008578682 @default.
- W3207493443 hasAuthorship W3207493443A5044722794 @default.