Matches in SemOpenAlex for { <https://semopenalex.org/work/W3207514143> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W3207514143 abstract "Inorganic nanocrystals such as quantum dots (QDs) and upconverting nanoparticles (UCNPs) are uniquely suited for quanti-tative live-cell imaging and are typically functionalized with ligands to study specific receptors or cellular targets. Antibod-ies (Ab) are among the most useful targeting reagents owing to their high affinities and specificities, but common nanocrys-tal labeling methods may orient Ab incorrectly, be reversible or denaturing, or lead to Ab-NP complexes too large for some applications. Here, we show that SpyCatcher proteins, which bind and spontaneously form covalent isopeptide bonds with cognate SpyTag peptides, can conjugate engineered Ab to nanoparticle surfaces with control over stability, orientation, and stoichiometry. Compact SpyCatcher-functionalized QDs and UCNPs may be labeled with short-chain variable fragment Ab (scFv) engineered to bind urokinase-type plasminogen activator receptors (uPAR) that are overexpressed in many human can-cers. Confocal imaging of anti-uPAR scFv-QD conjugates shows the Ab mediates specific binding and internalization by breast cancer cells expressing uPAR. Time-lapse imaging of photostable scFv-UCNP conjugates show that Ab binding caus-es uPAR internalization with a ∼20-minute half-life on the cell surface, and uPAR is internalized to endolysosomal com-partments distinct from general membrane stains and without significant recycling to the cell surface. The controlled and stable conjugation of engineered Ab to NPs enables targeting of diverse receptors for live-cell study of their distribution, trafficking, and physiology." @default.
- W3207514143 created "2021-10-25" @default.
- W3207514143 creator A5005415632 @default.
- W3207514143 creator A5016162499 @default.
- W3207514143 creator A5019879895 @default.
- W3207514143 creator A5022294046 @default.
- W3207514143 creator A5023545037 @default.
- W3207514143 creator A5027325495 @default.
- W3207514143 creator A5061536008 @default.
- W3207514143 creator A5087001996 @default.
- W3207514143 date "2021-10-15" @default.
- W3207514143 modified "2023-10-18" @default.
- W3207514143 title "Immunotargeting of Nanocrystals by SpyCatcher Conjugation of Engineered Antibodies" @default.
- W3207514143 doi "https://doi.org/10.33774/chemrxiv-2021-9q5xr" @default.
- W3207514143 hasPublicationYear "2021" @default.
- W3207514143 type Work @default.
- W3207514143 sameAs 3207514143 @default.
- W3207514143 citedByCount "0" @default.
- W3207514143 crossrefType "posted-content" @default.
- W3207514143 hasAuthorship W3207514143A5005415632 @default.
- W3207514143 hasAuthorship W3207514143A5016162499 @default.
- W3207514143 hasAuthorship W3207514143A5019879895 @default.
- W3207514143 hasAuthorship W3207514143A5022294046 @default.
- W3207514143 hasAuthorship W3207514143A5023545037 @default.
- W3207514143 hasAuthorship W3207514143A5027325495 @default.
- W3207514143 hasAuthorship W3207514143A5061536008 @default.
- W3207514143 hasAuthorship W3207514143A5087001996 @default.
- W3207514143 hasBestOaLocation W32075141431 @default.
- W3207514143 hasConcept C121608353 @default.
- W3207514143 hasConcept C12554922 @default.
- W3207514143 hasConcept C134306372 @default.
- W3207514143 hasConcept C139770010 @default.
- W3207514143 hasConcept C1491633281 @default.
- W3207514143 hasConcept C170493617 @default.
- W3207514143 hasConcept C185592680 @default.
- W3207514143 hasConcept C197336794 @default.
- W3207514143 hasConcept C28005876 @default.
- W3207514143 hasConcept C33923547 @default.
- W3207514143 hasConcept C54355233 @default.
- W3207514143 hasConcept C55493867 @default.
- W3207514143 hasConcept C56219504 @default.
- W3207514143 hasConcept C86803240 @default.
- W3207514143 hasConcept C95444343 @default.
- W3207514143 hasConcept C96232424 @default.
- W3207514143 hasConceptScore W3207514143C121608353 @default.
- W3207514143 hasConceptScore W3207514143C12554922 @default.
- W3207514143 hasConceptScore W3207514143C134306372 @default.
- W3207514143 hasConceptScore W3207514143C139770010 @default.
- W3207514143 hasConceptScore W3207514143C1491633281 @default.
- W3207514143 hasConceptScore W3207514143C170493617 @default.
- W3207514143 hasConceptScore W3207514143C185592680 @default.
- W3207514143 hasConceptScore W3207514143C197336794 @default.
- W3207514143 hasConceptScore W3207514143C28005876 @default.
- W3207514143 hasConceptScore W3207514143C33923547 @default.
- W3207514143 hasConceptScore W3207514143C54355233 @default.
- W3207514143 hasConceptScore W3207514143C55493867 @default.
- W3207514143 hasConceptScore W3207514143C56219504 @default.
- W3207514143 hasConceptScore W3207514143C86803240 @default.
- W3207514143 hasConceptScore W3207514143C95444343 @default.
- W3207514143 hasConceptScore W3207514143C96232424 @default.
- W3207514143 hasFunder F4320332161 @default.
- W3207514143 hasLocation W32075141431 @default.
- W3207514143 hasLocation W32075141432 @default.
- W3207514143 hasLocation W32075141433 @default.
- W3207514143 hasOpenAccess W3207514143 @default.
- W3207514143 hasPrimaryLocation W32075141431 @default.
- W3207514143 hasRelatedWork W1541110228 @default.
- W3207514143 hasRelatedWork W2026159277 @default.
- W3207514143 hasRelatedWork W2053998636 @default.
- W3207514143 hasRelatedWork W2063705161 @default.
- W3207514143 hasRelatedWork W2069093802 @default.
- W3207514143 hasRelatedWork W2125589729 @default.
- W3207514143 hasRelatedWork W2142925367 @default.
- W3207514143 hasRelatedWork W2221683966 @default.
- W3207514143 hasRelatedWork W3109609809 @default.
- W3207514143 hasRelatedWork W4244463151 @default.
- W3207514143 isParatext "false" @default.
- W3207514143 isRetracted "false" @default.
- W3207514143 magId "3207514143" @default.
- W3207514143 workType "article" @default.