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- W3207677334 abstract "Abstract Activated Gα q signals through Phospholipase-Cβ (PLCβ) and Trio, a Rho GTPase exchange factor (RhoGEF), but how these distinct effector pathways promote cellular responses to neurotransmitters like serotonin remains poorly understood. We used the egg-laying behavior circuit of C. elegans to determine whether PLCβ and Trio mediate serotonin and Gα q signaling through independent or related biochemical pathways. Using genetic rescue experiments, we find that PLCβ functions in neurons while Trio RhoGEF functions in both neurons and the postsynaptic vulval muscles. While Gα q , PLCβ, and Trio RhoGEF mutants all fail to lay eggs in response to serotonin, optogenetic stimulation of the serotonin releasing HSN command neurons restores egg laying only in PLCβ mutants. PLCβ mutants showed vulval muscle Ca 2+ transients while strong Gα q and Trio RhoGEF mutants had little or no vulval muscle Ca 2+ activity. Treatment with Phorbol esters that mimic Diacylglycerol (DAG), a product of PIP 2 hydrolysis, rescued egg-laying circuit activity and behavior defects of Gα q signaling mutants, suggesting both Phospholipase C and Rho signaling promote synaptic transmission and egg-laying via DAG production. DAG activates effectors including UNC-13, however, we find that phorbol esters, but not serotonin, stimulate egg laying in unc-13 and PLCβ mutants. These results support a model where serotonin signaling through Gα q , PLCβ, and UNC-13 promote neurotransmitter release, and that serotonin also signals through Gα q , Trio RhoGEF, and an unidentified PMA-responsive effector to promote postsynaptic muscle excitability. Thus, the same neuromodulator serotonin can signal in distinct cells and effector pathways to coordinate activation of a motor behavior circuit." @default.
- W3207677334 created "2021-10-25" @default.
- W3207677334 creator A5011774992 @default.
- W3207677334 creator A5018534393 @default.
- W3207677334 creator A5046821623 @default.
- W3207677334 date "2021-05-10" @default.
- W3207677334 modified "2023-10-16" @default.
- W3207677334 title "Serotonin signals through postsynaptic Gαq, Trio RhoGEF, and DAG to promote C. elegans egg-laying circuit activity and behavior" @default.
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