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- W3208010061 abstract "Human pluripotent stem cells (hPSC) can be directed to differentiate in vitro into insulin-prorducing beta cells (SC-β). Although these cells accurately respond to glucose and can reverse diabetes in preclinical models improvments in the final cell products are desirable. For example, safety, controlling the cellular compositions and protection against immune rejection may be addressed by genetic modifications of SC-β pre-transplantation. To screen for gene targets, we have generated a human embryonic stem cell line (hESC) that constitutively express the enhanced specificity Streptococcus pyogenes Cas9 (eSpCas9) gene, knocked-in into the GAPDH locus." @default.
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- W3208010061 date "2021-12-01" @default.
- W3208010061 modified "2023-09-24" @default.
- W3208010061 title "A human ESC line for efficient CRISPR editing of pluripotent stem cells" @default.
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- W3208010061 doi "https://doi.org/10.1016/j.scr.2021.102591" @default.
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