FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3208012308> ?p ?o ?g. }

• W3208012308 endingPage "326" @default.
• W3208012308 startingPage "315" @default.
• W3208012308 abstract "Tandem affinity purification is a useful strategy to isolate multisubunit complexes of high yield and purity but can be limited when working with halophilic proteins that are not properly expressed in Escherichia coli. Halophilic proteins are desirable for bioindustrial applications as they are often stable and active in organic solvents; however, these proteins can be difficult to express, fold, and purify by traditional technologies. Haloarchaea provide a useful alternative for expression of halophilic proteins. These microorganisms use a salt-in strategy to maintain homeostasis and express most of their proteins with halophilic properties and low pI. Here, we provide detailed protocols for the genetic modification, expression and tandem affinity purification of salt-loving multisubunit complexes from the haloarchaeon Haloferax volcanii. The strategy for isolation of affinity tagged 20S proteasomes that form cylindrical proteolytic nanomachines of α1, α2 and β subunits is described." @default.
• W3208012308 created "2021-11-08" @default.
• W3208012308 creator A5015444565 @default.
• W3208012308 creator A5017976361 @default.
• W3208012308 creator A5024566457 @default.
• W3208012308 creator A5026534526 @default.
• W3208012308 creator A5046436672 @default.
• W3208012308 creator A5052205852 @default.
• W3208012308 creator A5055225891 @default.
• W3208012308 date "2021-01-01" @default.
• W3208012308 modified "2023-10-17" @default.
• W3208012308 title "Expression and tandem affinity purification of 20S proteasomes and other multisubunit complexes in Haloferax volcanii" @default.
• W3208012308 cites W1845958951 @default.
• W3208012308 cites W1999870505 @default.
• W3208012308 cites W2009419872 @default.
• W3208012308 cites W2042718339 @default.
• W3208012308 cites W2043040963 @default.
• W3208012308 cites W2049771316 @default.
• W3208012308 cites W2059318369 @default.
• W3208012308 cites W2079263991 @default.
• W3208012308 cites W2084261089 @default.
• W3208012308 cites W2089673861 @default.
• W3208012308 cites W2099890007 @default.
• W3208012308 cites W2112589109 @default.
• W3208012308 cites W2115847027 @default.
• W3208012308 cites W2131792202 @default.
• W3208012308 cites W2146908577 @default.
• W3208012308 cites W2153238599 @default.
• W3208012308 cites W2408426418 @default.
• W3208012308 cites W659176249 @default.
• W3208012308 doi "https://doi.org/10.1016/bs.mie.2021.08.002" @default.
• W3208012308 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34752292" @default.
• W3208012308 hasPublicationYear "2021" @default.
• W3208012308 type Work @default.
• W3208012308 sameAs 3208012308 @default.
• W3208012308 citedByCount "3" @default.
• W3208012308 countsByYear W32080123082022 @default.
• W3208012308 countsByYear W32080123082023 @default.
• W3208012308 crossrefType "book-chapter" @default.
• W3208012308 hasAuthorship W3208012308A5015444565 @default.
• W3208012308 hasAuthorship W3208012308A5017976361 @default.
• W3208012308 hasAuthorship W3208012308A5024566457 @default.
• W3208012308 hasAuthorship W3208012308A5026534526 @default.
• W3208012308 hasAuthorship W3208012308A5046436672 @default.
• W3208012308 hasAuthorship W3208012308A5052205852 @default.
• W3208012308 hasAuthorship W3208012308A5055225891 @default.
• W3208012308 hasBestOaLocation W32080123082 @default.
• W3208012308 hasConcept C104317684 @default.
• W3208012308 hasConcept C159985019 @default.
• W3208012308 hasConcept C179247698 @default.
• W3208012308 hasConcept C181199279 @default.
• W3208012308 hasConcept C185592680 @default.
• W3208012308 hasConcept C192562407 @default.
• W3208012308 hasConcept C27740335 @default.
• W3208012308 hasConcept C2776179084 @default.
• W3208012308 hasConcept C2777814067 @default.
• W3208012308 hasConcept C2779926853 @default.
• W3208012308 hasConcept C523546767 @default.
• W3208012308 hasConcept C54355233 @default.
• W3208012308 hasConcept C547475151 @default.
• W3208012308 hasConcept C550995028 @default.
• W3208012308 hasConcept C55493867 @default.
• W3208012308 hasConcept C71095615 @default.
• W3208012308 hasConcept C80642116 @default.
• W3208012308 hasConcept C86803240 @default.
• W3208012308 hasConcept C95444343 @default.
• W3208012308 hasConceptScore W3208012308C104317684 @default.
• W3208012308 hasConceptScore W3208012308C159985019 @default.
• W3208012308 hasConceptScore W3208012308C179247698 @default.
• W3208012308 hasConceptScore W3208012308C181199279 @default.
• W3208012308 hasConceptScore W3208012308C185592680 @default.
• W3208012308 hasConceptScore W3208012308C192562407 @default.
• W3208012308 hasConceptScore W3208012308C27740335 @default.
• W3208012308 hasConceptScore W3208012308C2776179084 @default.
• W3208012308 hasConceptScore W3208012308C2777814067 @default.
• W3208012308 hasConceptScore W3208012308C2779926853 @default.
• W3208012308 hasConceptScore W3208012308C523546767 @default.
• W3208012308 hasConceptScore W3208012308C54355233 @default.
• W3208012308 hasConceptScore W3208012308C547475151 @default.
• W3208012308 hasConceptScore W3208012308C550995028 @default.
• W3208012308 hasConceptScore W3208012308C55493867 @default.
• W3208012308 hasConceptScore W3208012308C71095615 @default.
• W3208012308 hasConceptScore W3208012308C80642116 @default.
• W3208012308 hasConceptScore W3208012308C86803240 @default.
• W3208012308 hasConceptScore W3208012308C95444343 @default.
• W3208012308 hasLocation W32080123081 @default.
• W3208012308 hasLocation W32080123082 @default.
• W3208012308 hasLocation W32080123083 @default.
• W3208012308 hasOpenAccess W3208012308 @default.
• W3208012308 hasPrimaryLocation W32080123081 @default.
• W3208012308 hasRelatedWork W1998555791 @default.
• W3208012308 hasRelatedWork W2031018687 @default.
• W3208012308 hasRelatedWork W2142984901 @default.
• W3208012308 hasRelatedWork W2292557091 @default.
• W3208012308 hasRelatedWork W2754857012 @default.
• W3208012308 hasRelatedWork W3047005611 @default.
• W3208012308 hasRelatedWork W3120204839 @default.
• W3208012308 hasRelatedWork W4312454364 @default.

• next