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- W3208015703 abstract "Microorganisms play an essential role in sulfide removal. Alkaline absorption solution facilitates the sulfide's dissolution and oxidative degradation, so haloalkaliphile is a prospective source for environmental-friendly and cost-effective biodesulfurization. In this research, 484 sulfide oxidation genes were identified from the metagenomes of the soda-saline lakes and a haloalkaliphilic heterotrophic bacterium Halomonas salifodinae IM328 (=CGMCC 22183) was isolated from the same habitat as the host for expression of a representative sequence. The genetic manipulation was successfully achieved through the conjugation transformation method, and sulfide: quinone oxidoreductase gene (sqr) was expressed via pBBR1MCS derivative plasmid. Furthermore, a whole-cell catalyst system was developed by using the engineered strain that exhibited a higher rate of sulfide oxidation under the optimal alkaline pH of 9.0. The whole-cell catalyst could be recycled six times to maintain the sulfide oxidation rates from 41.451 to 80.216 µmol·min-1·g-1 dry cell mass. To summarize, a whole-cell catalyst system based on the engineered haloalkaliphilic bacterium is potentiated to be applied in the sulfide treatment at a reduced cost." @default.
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- W3208015703 date "2021-10-24" @default.
- W3208015703 modified "2023-10-16" @default.
- W3208015703 title "Development of whole-cell catalyst system for sulfide biotreatment based on the engineered haloalkaliphilic bacterium" @default.
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- W3208015703 doi "https://doi.org/10.1186/s13568-021-01302-9" @default.
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