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• W3208112887 endingPage "120532" @default.
• W3208112887 startingPage "120532" @default.
• W3208112887 abstract "Auramine o (AO) is a synthetic dye used in paper and textile industries. Although it has been an unauthorized food additive in many countries due to its toxic and carcinogenic possibility, its illegal uses have been detected in certain food products such as pasta, semolina and spices and also in pharmaceuticals. The presence of AO in food products should be monitored, therefore, to minimize the negative health effects on consumers. In this study, a simple, highly sensitive and selective label free detection method was investigated for AO by G-quadruplex-based fluorescent turn-on strategy. The optimum fluorescent detection assay was achieved with a specific G-quadruplex DNA sequence, c-myc, at 400 nM in Tris-HCl buffer at pH 7.4. The linearity of fluorescence intensity depending on AO concentration ranged from 0 to 0.07 µM and LOD and LOQ were 3 nM and 10 nM, respectively. The G-quadruplex-based detection assay was highly specific for AO as compared to other two synthetic food colorings and successfully applied to determine AO in pasta, bulgur and curry powder with recoveries in the range from 70.33% to 106.49%. This G-quadruplex-based label free detection assay has a significant potential to be used in the detection of AO in food products." @default.
• W3208112887 created "2021-11-08" @default.
• W3208112887 creator A5017124310 @default.
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• W3208112887 creator A5059550349 @default.
• W3208112887 date "2022-02-01" @default.
• W3208112887 modified "2023-09-25" @default.
• W3208112887 title "Label free detection of auramine O by G-quadruplex-based fluorescent turn-on strategy" @default.
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• W3208112887 doi "https://doi.org/10.1016/j.saa.2021.120532" @default.
• W3208112887 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34776374" @default.
• W3208112887 hasPublicationYear "2022" @default.
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