Matches in SemOpenAlex for { <https://semopenalex.org/work/W3208130009> ?p ?o ?g. }
- W3208130009 abstract "Discovery of novel enzymes is a challenging task, yet a crucial one, due to their increasing relevance as chemical catalysts and biotechnological tools. In our work we present a high-throughput screening approach to discovering novel activities. A screen of 96 putative oxidases with 23 substrates led to the discovery of two new enzymes. The first enzyme, N-acetyl-D-hexosamine oxidase (EC 1.1.3.29) from Ralstonia solanacearum, is a vanillyl alcohol oxidase-like flavoprotein displaying the highest activity with N-acetylglucosamine and N-acetylgalactosamine. Before our discovery of the enzyme, its activity was an orphan one - experimentally characterized but lacking the link to amino acid sequence. The second enzyme, from an uncultured marine euryarchaeota, is a long-chain alcohol oxidase (LCAO, EC 1.1.3.20) active with a range of fatty alcohols, with 1-dodecanol being the preferred substrate. The enzyme displays no sequence similarity to previously characterised LCAOs, and thus is a completely novel representative of a protein with such activity." @default.
- W3208130009 created "2021-11-08" @default.
- W3208130009 creator A5009350210 @default.
- W3208130009 creator A5025072659 @default.
- W3208130009 creator A5045677182 @default.
- W3208130009 creator A5083203989 @default.
- W3208130009 date "2021-11-18" @default.
- W3208130009 modified "2023-09-27" @default.
- W3208130009 title "Discovery of Two Novel Oxidases Using a High‐Throughput Activity Screen" @default.
- W3208130009 cites W1869761577 @default.
- W3208130009 cites W1947659320 @default.
- W3208130009 cites W1963578203 @default.
- W3208130009 cites W1969733451 @default.
- W3208130009 cites W1970051672 @default.
- W3208130009 cites W1981264193 @default.
- W3208130009 cites W1981759407 @default.
- W3208130009 cites W2000457174 @default.
- W3208130009 cites W2010111858 @default.
- W3208130009 cites W2033222415 @default.
- W3208130009 cites W2041837226 @default.
- W3208130009 cites W2043772385 @default.
- W3208130009 cites W2077089049 @default.
- W3208130009 cites W2078769202 @default.
- W3208130009 cites W2090881994 @default.
- W3208130009 cites W2120959948 @default.
- W3208130009 cites W2130060890 @default.
- W3208130009 cites W2144002130 @default.
- W3208130009 cites W2148144302 @default.
- W3208130009 cites W2152386236 @default.
- W3208130009 cites W2168498506 @default.
- W3208130009 cites W2169059428 @default.
- W3208130009 cites W2507123105 @default.
- W3208130009 cites W2566364344 @default.
- W3208130009 cites W2733542526 @default.
- W3208130009 cites W2762653773 @default.
- W3208130009 cites W2788762081 @default.
- W3208130009 cites W2804515387 @default.
- W3208130009 cites W2899887519 @default.
- W3208130009 cites W2902863550 @default.
- W3208130009 cites W2912206726 @default.
- W3208130009 cites W2922258649 @default.
- W3208130009 cites W2924412896 @default.
- W3208130009 cites W2945995693 @default.
- W3208130009 cites W2974929746 @default.
- W3208130009 cites W2976704092 @default.
- W3208130009 cites W3006003358 @default.
- W3208130009 cites W3013554928 @default.
- W3208130009 cites W3014908193 @default.
- W3208130009 cites W3027445043 @default.
- W3208130009 cites W3084107535 @default.
- W3208130009 cites W3087071818 @default.
- W3208130009 cites W3124859893 @default.
- W3208130009 cites W3135130381 @default.
- W3208130009 cites W3156458251 @default.
- W3208130009 cites W3177828909 @default.
- W3208130009 cites W3201292455 @default.
- W3208130009 cites W3203343200 @default.
- W3208130009 cites W3208130009 @default.
- W3208130009 cites W4206117801 @default.
- W3208130009 doi "https://doi.org/10.1002/cbic.202100510" @default.
- W3208130009 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34709726" @default.
- W3208130009 hasPublicationYear "2021" @default.
- W3208130009 type Work @default.
- W3208130009 sameAs 3208130009 @default.
- W3208130009 citedByCount "4" @default.
- W3208130009 countsByYear W32081300092021 @default.
- W3208130009 countsByYear W32081300092022 @default.
- W3208130009 countsByYear W32081300092023 @default.
- W3208130009 crossrefType "journal-article" @default.
- W3208130009 hasAuthorship W3208130009A5009350210 @default.
- W3208130009 hasAuthorship W3208130009A5025072659 @default.
- W3208130009 hasAuthorship W3208130009A5045677182 @default.
- W3208130009 hasAuthorship W3208130009A5083203989 @default.
- W3208130009 hasBestOaLocation W32081300092 @default.
- W3208130009 hasConcept C181199279 @default.
- W3208130009 hasConcept C185592680 @default.
- W3208130009 hasConcept C18903297 @default.
- W3208130009 hasConcept C2777289219 @default.
- W3208130009 hasConcept C51323132 @default.
- W3208130009 hasConcept C55493867 @default.
- W3208130009 hasConcept C70721500 @default.
- W3208130009 hasConcept C71995715 @default.
- W3208130009 hasConcept C86803240 @default.
- W3208130009 hasConceptScore W3208130009C181199279 @default.
- W3208130009 hasConceptScore W3208130009C185592680 @default.
- W3208130009 hasConceptScore W3208130009C18903297 @default.
- W3208130009 hasConceptScore W3208130009C2777289219 @default.
- W3208130009 hasConceptScore W3208130009C51323132 @default.
- W3208130009 hasConceptScore W3208130009C55493867 @default.
- W3208130009 hasConceptScore W3208130009C70721500 @default.
- W3208130009 hasConceptScore W3208130009C71995715 @default.
- W3208130009 hasConceptScore W3208130009C86803240 @default.
- W3208130009 hasIssue "2" @default.
- W3208130009 hasLocation W32081300091 @default.
- W3208130009 hasLocation W32081300092 @default.
- W3208130009 hasLocation W32081300093 @default.
- W3208130009 hasLocation W32081300094 @default.
- W3208130009 hasLocation W32081300095 @default.
- W3208130009 hasOpenAccess W3208130009 @default.
- W3208130009 hasPrimaryLocation W32081300091 @default.