Matches in SemOpenAlex for { <https://semopenalex.org/work/W3208146006> ?p ?o ?g. }
- W3208146006 endingPage "S30" @default.
- W3208146006 startingPage "S19" @default.
- W3208146006 abstract "The treatment of such primary brain tumours such as glioblastoma multiforme (GBM) presents numerous difficulties, considerably impacting patient survival rates. The search for new compounds with therapeutic potential focuses on the identification of molecules that can be produced through chemical and enzymatic processes and that present anticancer effects in different types of tumours. Given the need for new treatments for GBM, we conducted a study to synthesise ortho-coumaric acid alkyl esters (OCAAE) and to evaluate them in an appropriate study model, a three-dimensional (3D) culture using the Matrigel extracellular matrix. We tested cytotoxicity with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) technique and by determining phosphatidylserine externalisation with annexin V and active caspase-3 expression as markers of apoptosis, and the subsequent degradation of DNA as a marker of cell death. Medium-chain OCAAEs, such as butyl-o-coumarate (BOC) and its isomer, showed the greatest effect on most of the parameters evaluated in 3D cultures of GBM, followed by methyl-, propyl-, and ethyl-o-coumarate (MOC, POC and EOC); these compounds showed effects both in reducing cell viability and in increasing the expression of active caspase-3 and annexin V and the degradation of DNA, in comparison with the first-line treatment for GBM, temozolomide. These results, together with those of previous researchers, show that the different OCAAEs have a potential therapeutic effect on GBM, and that 3D culture of GBM cells is an appropriate model for the study of new antitumour molecules. Los tumores cerebrales primarios como el Glioblastoma multiforme (GBM) presentan muchas dificultades para su tratamiento, lo que repercute considerablemente en la tasa de supervivencia del paciente. La búsqueda de nuevos compuestos con potencial terapéutico se enfoca en la identificación de moléculas, mismas que pueden ser producidas química y enzimáticamente; y que muestren efectos anticancerígenos en distintos tipos de tumores. La necesidad de nuevos tratamientos para el GBM, nos lleva a sintetizar alquil-ésteres del ácido orto-cumárico (ae-AOC) y a evaluarlos en un modelo de estudio apropiado como es el cultivo en tercera dimensión (3D) empleando la matriz extracelular, Matrigel™, mediante pruebas de citotoxicidad celular como el Bromuro de 3-(4,5-dimetiltiazol-2-il)-2,5-difenil tetrazolio (MTT), externalización de la fosfatidilserina, con Anexina V y la expresión de caspasa-3 activa como marcadores de apoptosis y la subsecuente degradación de ácido desoxirribonucleico (ADN) como marcador de muerte celular. Los ae-AOC de cadena mediana, como el butil-o-cumarato (BOC) y su isómero, presentaron un efecto significativo mayor en la mayoría de los parámetros evaluados en los cultivos en 3D de GBM, siguiendo de forma significativa el metil, el propil y el etil-o-cumarato (MOC, POC y EOC), presentaron efectos tanto en la reducción de la viabilidad celular como en el incremento en la expresión de caspasa-3 activa, Anexina V y la degradación del ADN, en comparación con el tratamiento de primera línea para los GBM, temozolomida (TMZ). Estos resultados, aunados a investigaciones previas, muestran que los diferentes ae-AOC poseen un potencial efecto terapéutico, en los GBM, y que el cultivo de células de GBM en 3D es un modelo apropiado para el estudio de nuevas moléculas antitumorales." @default.
- W3208146006 created "2021-11-08" @default.
- W3208146006 creator A5005952967 @default.
- W3208146006 creator A5035554590 @default.
- W3208146006 creator A5039591376 @default.
- W3208146006 creator A5046930571 @default.
- W3208146006 creator A5053533565 @default.
- W3208146006 creator A5058101103 @default.
- W3208146006 creator A5059840801 @default.
- W3208146006 creator A5063236840 @default.
- W3208146006 creator A5075480558 @default.
- W3208146006 creator A5081232027 @default.
- W3208146006 date "2022-01-01" @default.
- W3208146006 modified "2023-10-14" @default.
- W3208146006 title "Ortho-coumaric acid derivatives with therapeutic potential in a three-dimensional culture of the immortalised U-138 MG glioblastoma multiforme cell line" @default.
- W3208146006 cites W1966025347 @default.
- W3208146006 cites W2005647357 @default.
- W3208146006 cites W2011021455 @default.
- W3208146006 cites W2044419292 @default.
- W3208146006 cites W2054786345 @default.
- W3208146006 cites W2070336923 @default.
- W3208146006 cites W2071823612 @default.
- W3208146006 cites W2114959070 @default.
- W3208146006 cites W2127177010 @default.
- W3208146006 cites W2566129582 @default.
- W3208146006 cites W2588102465 @default.
- W3208146006 cites W2737738665 @default.
- W3208146006 cites W2768388636 @default.
- W3208146006 cites W2791853559 @default.
- W3208146006 cites W2792673537 @default.
- W3208146006 cites W2797877977 @default.
- W3208146006 cites W2802398159 @default.
- W3208146006 cites W2804593075 @default.
- W3208146006 cites W2805590406 @default.
- W3208146006 cites W2807364687 @default.
- W3208146006 cites W2890744696 @default.
- W3208146006 cites W2894254404 @default.
- W3208146006 cites W2911364339 @default.
- W3208146006 cites W2913156404 @default.
- W3208146006 cites W2954327576 @default.
- W3208146006 cites W2996811917 @default.
- W3208146006 cites W3024500390 @default.
- W3208146006 cites W3026094488 @default.
- W3208146006 cites W3043281228 @default.
- W3208146006 cites W3159362039 @default.
- W3208146006 cites W4211220688 @default.
- W3208146006 cites W765788331 @default.
- W3208146006 doi "https://doi.org/10.1016/j.neurop.2021.09.006" @default.
- W3208146006 hasPublicationYear "2022" @default.
- W3208146006 type Work @default.
- W3208146006 sameAs 3208146006 @default.
- W3208146006 citedByCount "2" @default.
- W3208146006 countsByYear W32081460062022 @default.
- W3208146006 countsByYear W32081460062023 @default.
- W3208146006 crossrefType "journal-article" @default.
- W3208146006 hasAuthorship W3208146006A5005952967 @default.
- W3208146006 hasAuthorship W3208146006A5035554590 @default.
- W3208146006 hasAuthorship W3208146006A5039591376 @default.
- W3208146006 hasAuthorship W3208146006A5046930571 @default.
- W3208146006 hasAuthorship W3208146006A5053533565 @default.
- W3208146006 hasAuthorship W3208146006A5058101103 @default.
- W3208146006 hasAuthorship W3208146006A5059840801 @default.
- W3208146006 hasAuthorship W3208146006A5063236840 @default.
- W3208146006 hasAuthorship W3208146006A5075480558 @default.
- W3208146006 hasAuthorship W3208146006A5081232027 @default.
- W3208146006 hasBestOaLocation W32081460061 @default.
- W3208146006 hasConcept C143998085 @default.
- W3208146006 hasConcept C185592680 @default.
- W3208146006 hasConcept C198352243 @default.
- W3208146006 hasConcept C2524010 @default.
- W3208146006 hasConcept C2776194525 @default.
- W3208146006 hasConcept C2778247072 @default.
- W3208146006 hasConcept C2780213334 @default.
- W3208146006 hasConcept C33923547 @default.
- W3208146006 hasConcept C502942594 @default.
- W3208146006 hasConcept C54355233 @default.
- W3208146006 hasConcept C55493867 @default.
- W3208146006 hasConcept C71924100 @default.
- W3208146006 hasConcept C81885089 @default.
- W3208146006 hasConcept C86803240 @default.
- W3208146006 hasConceptScore W3208146006C143998085 @default.
- W3208146006 hasConceptScore W3208146006C185592680 @default.
- W3208146006 hasConceptScore W3208146006C198352243 @default.
- W3208146006 hasConceptScore W3208146006C2524010 @default.
- W3208146006 hasConceptScore W3208146006C2776194525 @default.
- W3208146006 hasConceptScore W3208146006C2778247072 @default.
- W3208146006 hasConceptScore W3208146006C2780213334 @default.
- W3208146006 hasConceptScore W3208146006C33923547 @default.
- W3208146006 hasConceptScore W3208146006C502942594 @default.
- W3208146006 hasConceptScore W3208146006C54355233 @default.
- W3208146006 hasConceptScore W3208146006C55493867 @default.
- W3208146006 hasConceptScore W3208146006C71924100 @default.
- W3208146006 hasConceptScore W3208146006C81885089 @default.
- W3208146006 hasConceptScore W3208146006C86803240 @default.
- W3208146006 hasLocation W32081460061 @default.
- W3208146006 hasOpenAccess W3208146006 @default.
- W3208146006 hasPrimaryLocation W32081460061 @default.
- W3208146006 hasRelatedWork W2158418013 @default.