FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3208177169> ?p ?o ?g. }

• W3208177169 abstract "Bacterial natural products in general, and non-ribosomally synthesized peptides in particular, are structurally diverse and provide us with a broad range of pharmaceutically relevant bioactivities. Yet, traditional natural product research suffers from rediscovering the same scaffolds and has been stigmatised as inefficient, time-, labour-, and cost-intensive. Combinatorial chemistry, on the other hand, can produce new molecules in greater numbers, cheaper and in less time than traditional natural product discovery, but also fails to meet current medical needs due to the limited biologically relevant chemical space that can be addressed. Consequently, methods for the high throughput generation of new-to-nature natural products would offer a new approach to identifying novel bioactive chemical entities for the hit to lead phase of drug discovery programms. As a follow-up to our previously published proof-of-principle study on generating bipartite type S non-ribosomal peptide synthetases (NRPSs), we now envisaged the de novo generation of non-ribosomal peptides (NRPs) on an unreached scale. Using synthetic zippers, we split NRPS in up to three subunits and rapidly generated different bi- and tripartite NRPS libraries to produce 49 peptides, peptide derivatives, and de novo peptides at good titres up to 145 mgL-1. A further advantage of type S NRPSs not only is the possibility to easily expand the created libraries by re-using previously created type S NRPS, but that functions of individual domains as well as domain-domain interactions can be studied and assigned rapidly." @default.
• W3208177169 created "2021-11-08" @default.
• W3208177169 creator A5028638969 @default.
• W3208177169 creator A5031730861 @default.
• W3208177169 creator A5064841491 @default.
• W3208177169 creator A5070546959 @default.
• W3208177169 creator A5077926795 @default.
• W3208177169 date "2021-10-26" @default.
• W3208177169 modified "2023-09-23" @default.
• W3208177169 title "Type S Non Ribosomal Peptide Synthetases for the rapid generation of tailor-made peptide libraries" @default.
• W3208177169 cites W1964141703 @default.
• W3208177169 cites W1990244468 @default.
• W3208177169 cites W1991803593 @default.
• W3208177169 cites W2003127175 @default.
• W3208177169 cites W2004762726 @default.
• W3208177169 cites W2006137883 @default.
• W3208177169 cites W2007343995 @default.
• W3208177169 cites W2012251902 @default.
• W3208177169 cites W2014095623 @default.
• W3208177169 cites W2018409138 @default.
• W3208177169 cites W2023192368 @default.
• W3208177169 cites W2027277404 @default.
• W3208177169 cites W2033588393 @default.
• W3208177169 cites W2034769438 @default.
• W3208177169 cites W2046364391 @default.
• W3208177169 cites W2047695364 @default.
• W3208177169 cites W2056747336 @default.
• W3208177169 cites W2066344769 @default.
• W3208177169 cites W2067489282 @default.
• W3208177169 cites W2070072813 @default.
• W3208177169 cites W2073531367 @default.
• W3208177169 cites W2088226047 @default.
• W3208177169 cites W2095842646 @default.
• W3208177169 cites W2103090173 @default.
• W3208177169 cites W2105820587 @default.
• W3208177169 cites W2106605095 @default.
• W3208177169 cites W2115744127 @default.
• W3208177169 cites W212599732 @default.
• W3208177169 cites W2137631963 @default.
• W3208177169 cites W2138298207 @default.
• W3208177169 cites W2141030171 @default.
• W3208177169 cites W2163380920 @default.
• W3208177169 cites W2170928471 @default.
• W3208177169 cites W2189116376 @default.
• W3208177169 cites W2189137321 @default.
• W3208177169 cites W2222088233 @default.
• W3208177169 cites W2346680365 @default.
• W3208177169 cites W2409814849 @default.
• W3208177169 cites W2517082156 @default.
• W3208177169 cites W2602946330 @default.
• W3208177169 cites W2607335957 @default.
• W3208177169 cites W2609551972 @default.
• W3208177169 cites W2611021352 @default.
• W3208177169 cites W2769533173 @default.
• W3208177169 cites W2772427550 @default.
• W3208177169 cites W2885391792 @default.
• W3208177169 cites W2934492351 @default.
• W3208177169 cites W2942829220 @default.
• W3208177169 cites W2948447106 @default.
• W3208177169 cites W2951089481 @default.
• W3208177169 cites W2953174153 @default.
• W3208177169 cites W2983327530 @default.
• W3208177169 cites W2987939695 @default.
• W3208177169 cites W2988553559 @default.
• W3208177169 cites W2993803840 @default.
• W3208177169 cites W3091691794 @default.
• W3208177169 cites W3119952098 @default.
• W3208177169 cites W3154123611 @default.
• W3208177169 cites W3159117888 @default.
• W3208177169 cites W3163687632 @default.
• W3208177169 cites W3194188670 @default.
• W3208177169 cites W3195169074 @default.
• W3208177169 doi "https://doi.org/10.1101/2021.10.25.465728" @default.
• W3208177169 hasPublicationYear "2021" @default.
• W3208177169 type Work @default.
• W3208177169 sameAs 3208177169 @default.
• W3208177169 citedByCount "0" @default.
• W3208177169 crossrefType "posted-content" @default.
• W3208177169 hasAuthorship W3208177169A5028638969 @default.
• W3208177169 hasAuthorship W3208177169A5031730861 @default.
• W3208177169 hasAuthorship W3208177169A5064841491 @default.
• W3208177169 hasAuthorship W3208177169A5070546959 @default.
• W3208177169 hasAuthorship W3208177169A5077926795 @default.
• W3208177169 hasBestOaLocation W32081771691 @default.
• W3208177169 hasConcept C104317684 @default.
• W3208177169 hasConcept C185592680 @default.
• W3208177169 hasConcept C191908910 @default.
• W3208177169 hasConcept C21951064 @default.
• W3208177169 hasConcept C2779281246 @default.
• W3208177169 hasConcept C2779396153 @default.
• W3208177169 hasConcept C2909999020 @default.
• W3208177169 hasConcept C41008148 @default.
• W3208177169 hasConcept C55493867 @default.
• W3208177169 hasConcept C67705224 @default.
• W3208177169 hasConcept C67905577 @default.
• W3208177169 hasConcept C70721500 @default.
• W3208177169 hasConcept C74187038 @default.
• W3208177169 hasConcept C86803240 @default.
• W3208177169 hasConcept C88478588 @default.
• W3208177169 hasConcept C99726746 @default.

• next