FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3208239795> ?p ?o ?g. }

• W3208239795 abstract "To observe the effect of electroacupuncture (EA) combined with Zhuang-medicine-thread moxibustion on silent information regulator-1 (SIRT1)/nuclear factor kappa B (NF-κB) signal pathway and inflammatory factor expression in gastric antrum tissue of diabetic gastroparesis (DGP) rats, so as to explore its mechanism underlying improvement of DGP.Male SD rats were randomly divided into normal, model, medication, EA, Zhuang-medicine-thread moxibustion (moxibustion) and EA+moxibustion groups (n=12 per group). The DGP model was established by intraperitoneal injection of streptozotocin (STZ). Rats of the medication group were treated by gavage of 0.15 mg/mL mosapride citrate suspension. EA (10 Hz /50 Hz, 2 mA) or moxibustion (3 cones) or EA+moxibustion was applied to Zhongwan(CV12), bilateral Neiguan(PC6) and bilateral Sanyinjiao(SP6) of the related group for 20 min, once a day for 3 weeks. Blood glucose, gastric emptying rate and intestinal propulsion rate were measured. The levels of serum interleukin (IL)-6, IL-8, IL-10 and tumor necrosis factor-α(TNF-α) were detected by ELISA; the phosphorylation level of the phosphorylated inhibitor of nuclear factor κBα inhibitor (pIκ-Bα), the protein and mRNA expression of NF-κB p65 and SIRT1 in the gastric antrum tissue were detected by Western blot and real-time quantifitative PCR, respectively.(1) Compared with the normal group, the levels of blood glucose, serum IL-6, IL-8, TNF-α, and gastric pIκ-Bα and NF-κB p65 protein and mRNA expressions were significantly increased (P<0.01), and the gastric emptying rate, intestinal propulsion rate, serum IL-10 level, and SIRT1 protein and mRNA expressions were considerably decreased in the model group (P<0.01). (2) In contrast to the model group, the blood glucose in the EA, moxibustion and EA+moxibustion groups, serum IL-6, IL-8 and TNF-α levels in the 4 treatment groups, as well as NF-κB p65 protein expression in the medication and EA+moxibustion groups, and NF-κB p65 mRNA expression and pIκ-Bα protein and mRNA expression in the 4 treatment groups were significantly decreased (P<0.01, P<0.05); while the gastric emptying rate and intestinal propulsive rate and IL-10 content in the 4 treatment groups, and SIRT1 protein and mRNA expression in the medication and EA+moxibustion groups were obviously increased (P<0.05, P<0.01). (3) The effects of EA+moxibustion were significantly superior to those of simple EA and moxibustion in increasing gastric emptying rate, IL-10, SIRT1 protein expression (P<0.05, P<0.01), and in lowering IL-8 and TNF-α contents, pIκ-Bα protein and mRNA expression and NF-κB p65 mRNA expression (P<0.05, P<0.01). No significant differences were found among the 4 intervention groups in promoting the intestinal propulsive rate and among the EA, moxibustion and EA+moxibustion groups in lowering blood glucose (P>0.05).EA combined with Zhuang-medicine-thread moxibustion can effectively reduce the level of serum inflammatory factors and regulate SIRT1/NF-κB signal pathway in DGP rats, which may contribute to its function in improving gastrointestinal movement.目的：观察电针联合壮医药线点灸对糖尿病胃轻瘫(DGP)大鼠胃窦组织沉默信息调节因子-1(SIRT1)/核因子-κB(NF-κB)信号通路及炎性因子表达的影响，探讨电针联合壮医药线点灸治疗DGP的作用机制。方法：SD大鼠随机分为正常组、模型组、西药组、电针组、点灸组、观察组，每组12只。采用链脲佐菌素腹腔注射构建DGP模型。西药组予0.15 mg/mL枸橼酸莫沙必利混悬液灌胃给药，电针组和点灸组均选取“中脘”“内关”“三阴交”，电针组给予电针20 min，点灸组每穴点灸3壮，观察组给予电针联合点灸治疗，取穴及操作同电针组和点灸组。各组治疗均每日1次，治疗3周。测定各组大鼠血糖、胃排空率、小肠推进率；ELISA法检测血清白细胞介素(IL)-6、IL-8、IL-10、肿瘤坏死因子-α(TNF-α)含量；Western blot、荧光定量-PCR法检测胃窦组织磷酸化核因子κB抑制蛋白α亚基(pIκ-Bα)、NF-κB p65、SIRT1蛋白及mRNA的表达。结果：与正常组比较，模型组大鼠血糖，血清IL-6、IL-8、TNF-α含量，胃窦组织pIκ-Bα、NF-κB p65蛋白及mRNA表达均升高(P<0.01)，胃排空率、小肠推进率、血清IL-10含量、胃窦组织SIRT1蛋白及mRNA表达均降低(P<0.01)。与模型组比较，电针组、点灸组、观察组大鼠血糖降低(P<0.01)；所有治疗组胃排空率、小肠推进率、血清IL-10含量均升高(P<0.01，P <0.05)，血清IL-6、IL-8、TNF-α含量，胃窦组织pIκ-Bα蛋白及mRNA、NF-κB p65 mRNA表达均下降(P<0.01)；西药组及观察组胃窦组织NF-κB p65蛋白表达降低(P<0.05)，SIRT1蛋白及mRNA表达升高(P<0.01)。与电针组比较，观察组胃排空率、小肠推进率、血清IL-10含量、胃窦组织SIRT1 mRNA及基因表达均上升(P<0.05，P<0.01)，血清IL-8、TNF-α 含量，胃窦组织pIκ-Bα蛋白及pIκ-Bα、NF-κB p65 mRNA表达降低(P<0.01，P<0.05)。与点灸组比较，观察组胃排空率、小肠推进率、血清IL-10含量、胃窦组织SIRT1 mRNA表达升高(P<0.05，P<0.01)，血清IL-6、IL-8、TNF-α含量，胃窦组织pIκ-Bα蛋白及pIκ-Bα、NF-κB p65 mRNA表达降低(P<0.01, P<0.05)。结论：电针联合壮医药线点灸能有效降低DGP大鼠血清炎性因子水平，有效调控 SIRT1/NF-κB信号通路，这可能是其改善DGP胃肠道症状的作用机制之一。." @default.
• W3208239795 created "2021-11-08" @default.
• W3208239795 creator A5007321996 @default.
• W3208239795 creator A5023285263 @default.
• W3208239795 creator A5034428379 @default.
• W3208239795 creator A5035827582 @default.
• W3208239795 creator A5039830477 @default.
• W3208239795 creator A5042584561 @default.
• W3208239795 creator A5052099990 @default.
• W3208239795 creator A5084290308 @default.
• W3208239795 date "2021-10-25" @default.
• W3208239795 modified "2023-10-03" @default.
• W3208239795 title "[Effect of electroacupuncture combined with Zhuang-medicine-thread moxibustion on silent information regulator-1/nuclear factor κB signaling pathway in gastric antrum of diabetic gastroparesis rats]." @default.
• W3208239795 doi "https://doi.org/10.13702/j.1000-0607.200942" @default.
• W3208239795 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34698457" @default.
• W3208239795 hasPublicationYear "2021" @default.
• W3208239795 type Work @default.
• W3208239795 sameAs 3208239795 @default.
• W3208239795 citedByCount "0" @default.
• W3208239795 crossrefType "journal-article" @default.
• W3208239795 hasAuthorship W3208239795A5007321996 @default.
• W3208239795 hasAuthorship W3208239795A5023285263 @default.
• W3208239795 hasAuthorship W3208239795A5034428379 @default.
• W3208239795 hasAuthorship W3208239795A5035827582 @default.
• W3208239795 hasAuthorship W3208239795A5039830477 @default.
• W3208239795 hasAuthorship W3208239795A5042584561 @default.
• W3208239795 hasAuthorship W3208239795A5052099990 @default.
• W3208239795 hasAuthorship W3208239795A5084290308 @default.
• W3208239795 hasConcept C126322002 @default.
• W3208239795 hasConcept C131253125 @default.
• W3208239795 hasConcept C134018914 @default.
• W3208239795 hasConcept C142724271 @default.
• W3208239795 hasConcept C17991360 @default.
• W3208239795 hasConcept C204787440 @default.
• W3208239795 hasConcept C2776202274 @default.
• W3208239795 hasConcept C2777674692 @default.
• W3208239795 hasConcept C2779422922 @default.
• W3208239795 hasConcept C2779504368 @default.
• W3208239795 hasConcept C3020479747 @default.
• W3208239795 hasConcept C56837625 @default.
• W3208239795 hasConcept C71924100 @default.
• W3208239795 hasConceptScore W3208239795C126322002 @default.
• W3208239795 hasConceptScore W3208239795C131253125 @default.
• W3208239795 hasConceptScore W3208239795C134018914 @default.
• W3208239795 hasConceptScore W3208239795C142724271 @default.
• W3208239795 hasConceptScore W3208239795C17991360 @default.
• W3208239795 hasConceptScore W3208239795C204787440 @default.
• W3208239795 hasConceptScore W3208239795C2776202274 @default.
• W3208239795 hasConceptScore W3208239795C2777674692 @default.
• W3208239795 hasConceptScore W3208239795C2779422922 @default.
• W3208239795 hasConceptScore W3208239795C2779504368 @default.
• W3208239795 hasConceptScore W3208239795C3020479747 @default.
• W3208239795 hasConceptScore W3208239795C56837625 @default.
• W3208239795 hasConceptScore W3208239795C71924100 @default.
• W3208239795 hasLocation W32082397951 @default.
• W3208239795 hasOpenAccess W3208239795 @default.
• W3208239795 hasPrimaryLocation W32082397951 @default.
• W3208239795 hasRelatedWork W13013805 @default.
• W3208239795 hasRelatedWork W16791344 @default.
• W3208239795 hasRelatedWork W21040569 @default.
• W3208239795 hasRelatedWork W522966 @default.
• W3208239795 hasRelatedWork W8818755 @default.
• W3208239795 hasRelatedWork W9113744 @default.
• W3208239795 hasRelatedWork W9904803 @default.
• W3208239795 hasRelatedWork W14094381 @default.
• W3208239795 hasRelatedWork W16911764 @default.
• W3208239795 hasRelatedWork W880080 @default.
• W3208239795 isParatext "false" @default.
• W3208239795 isRetracted "false" @default.
• W3208239795 magId "3208239795" @default.
• W3208239795 workType "article" @default.