Matches in SemOpenAlex for { <https://semopenalex.org/work/W3208271136> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W3208271136 endingPage "44" @default.
- W3208271136 startingPage "44" @default.
- W3208271136 abstract "Conventional solvent-based precipitation makes it challenging to obtain a high recovery of low mass peptides. However, we previously demonstrated that the inclusion of salt ions, specifically ZnSO4, together with high concentrations of acetone, maximizes the recovery of peptides generated from trypsin digestion. We herein generalized this protocol to the rapid (5 min) precipitation of pepsin-digested peptides recovered from acidic matrices. The precipitation protocol extended to other organic solvents (acetonitrile), with high recovery from dilute peptide samples permitting preconcentration and purification. Mass spectrometry profiling of pepsin-generated peptides demonstrated that the protocol captured peptides as small as 800 u, although with a preferential bias towards recovering larger and more hydrophobic peptides. The precipitation protocol was applied to rapidly quench, concentrate, and purify pepsin-digested samples ahead of MS. Complex mixtures of yeast and plasma proteome extracts were successfully precipitated following digestion, with over 95% of MS-identified peptides observed in the pellet fraction. The full precipitation workflow-including the digestion step-can be completed in under 10 min, with direct MS analysis of the recovered peptide pellets showing exceptional protein sequence coverage." @default.
- W3208271136 created "2021-11-08" @default.
- W3208271136 creator A5009836701 @default.
- W3208271136 creator A5050134085 @default.
- W3208271136 creator A5090752332 @default.
- W3208271136 date "2021-11-03" @default.
- W3208271136 modified "2023-10-14" @default.
- W3208271136 title "Salt-Mediated Organic Solvent Precipitation for Enhanced Recovery of Peptides Generated by Pepsin Digestion" @default.
- W3208271136 cites W102715436 @default.
- W3208271136 cites W1491180863 @default.
- W3208271136 cites W1981417774 @default.
- W3208271136 cites W2002931786 @default.
- W3208271136 cites W2010940561 @default.
- W3208271136 cites W2012366627 @default.
- W3208271136 cites W2035629904 @default.
- W3208271136 cites W2046399369 @default.
- W3208271136 cites W2075584791 @default.
- W3208271136 cites W2084556466 @default.
- W3208271136 cites W2088793589 @default.
- W3208271136 cites W2089503717 @default.
- W3208271136 cites W2095867043 @default.
- W3208271136 cites W2139076700 @default.
- W3208271136 cites W2747664943 @default.
- W3208271136 cites W2896121881 @default.
- W3208271136 cites W2951852411 @default.
- W3208271136 cites W3011682025 @default.
- W3208271136 cites W3082082989 @default.
- W3208271136 doi "https://doi.org/10.3390/proteomes9040044" @default.
- W3208271136 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8628918" @default.
- W3208271136 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34842819" @default.
- W3208271136 hasPublicationYear "2021" @default.
- W3208271136 type Work @default.
- W3208271136 sameAs 3208271136 @default.
- W3208271136 citedByCount "3" @default.
- W3208271136 countsByYear W32082711362022 @default.
- W3208271136 countsByYear W32082711362023 @default.
- W3208271136 crossrefType "journal-article" @default.
- W3208271136 hasAuthorship W3208271136A5009836701 @default.
- W3208271136 hasAuthorship W3208271136A5050134085 @default.
- W3208271136 hasAuthorship W3208271136A5090752332 @default.
- W3208271136 hasBestOaLocation W32082711361 @default.
- W3208271136 hasConcept C107054158 @default.
- W3208271136 hasConcept C121332964 @default.
- W3208271136 hasConcept C153294291 @default.
- W3208271136 hasConcept C162356407 @default.
- W3208271136 hasConcept C16311235 @default.
- W3208271136 hasConcept C178790620 @default.
- W3208271136 hasConcept C181199279 @default.
- W3208271136 hasConcept C185592680 @default.
- W3208271136 hasConcept C2776371256 @default.
- W3208271136 hasConcept C2779281246 @default.
- W3208271136 hasConcept C2780340462 @default.
- W3208271136 hasConcept C2780471494 @default.
- W3208271136 hasConcept C2781357212 @default.
- W3208271136 hasConcept C43617362 @default.
- W3208271136 hasConcept C55493867 @default.
- W3208271136 hasConcept C74774607 @default.
- W3208271136 hasConceptScore W3208271136C107054158 @default.
- W3208271136 hasConceptScore W3208271136C121332964 @default.
- W3208271136 hasConceptScore W3208271136C153294291 @default.
- W3208271136 hasConceptScore W3208271136C162356407 @default.
- W3208271136 hasConceptScore W3208271136C16311235 @default.
- W3208271136 hasConceptScore W3208271136C178790620 @default.
- W3208271136 hasConceptScore W3208271136C181199279 @default.
- W3208271136 hasConceptScore W3208271136C185592680 @default.
- W3208271136 hasConceptScore W3208271136C2776371256 @default.
- W3208271136 hasConceptScore W3208271136C2779281246 @default.
- W3208271136 hasConceptScore W3208271136C2780340462 @default.
- W3208271136 hasConceptScore W3208271136C2780471494 @default.
- W3208271136 hasConceptScore W3208271136C2781357212 @default.
- W3208271136 hasConceptScore W3208271136C43617362 @default.
- W3208271136 hasConceptScore W3208271136C55493867 @default.
- W3208271136 hasConceptScore W3208271136C74774607 @default.
- W3208271136 hasFunder F4320334593 @default.
- W3208271136 hasIssue "4" @default.
- W3208271136 hasLocation W32082711361 @default.
- W3208271136 hasLocation W32082711362 @default.
- W3208271136 hasLocation W32082711363 @default.
- W3208271136 hasLocation W32082711364 @default.
- W3208271136 hasOpenAccess W3208271136 @default.
- W3208271136 hasPrimaryLocation W32082711361 @default.
- W3208271136 hasRelatedWork W2069072339 @default.
- W3208271136 hasRelatedWork W2069126794 @default.
- W3208271136 hasRelatedWork W2167062834 @default.
- W3208271136 hasRelatedWork W2321178541 @default.
- W3208271136 hasRelatedWork W2950761424 @default.
- W3208271136 hasRelatedWork W3123603841 @default.
- W3208271136 hasRelatedWork W4225138533 @default.
- W3208271136 hasRelatedWork W4251993841 @default.
- W3208271136 hasRelatedWork W4310739394 @default.
- W3208271136 hasRelatedWork W93161100 @default.
- W3208271136 hasVolume "9" @default.
- W3208271136 isParatext "false" @default.
- W3208271136 isRetracted "false" @default.
- W3208271136 magId "3208271136" @default.
- W3208271136 workType "article" @default.