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- W3208390229 abstract "Plant pathology has been revolutionized by the emergence and intervention of next-generation sequencing technologies (NGS) which provide a fast, cost-effective, and reliable diagnostic for any class of pathogens. NGS has made tremendous advancements in the area of research and diagnostics of plant infecting viromes and has bridged plant virology with other advanced research fields like genome editing technologies. NGS in a broader perspective holds the potential for plant health improvement by diagnosing and mitigating the new or unusual symptoms caused by novel/unidentified viruses. CRISPR-based genome editing technologies can enable rapid engineering of efficient viral/viroid resistance by directly targeting specific nucleotide sites of plant viruses and viroids. Critical genes such as eIf (iso) 4E or eIF4E have been targeted via the CRISPR platform to produce plants resistant to single-stranded RNA (ssRNA) viruses. CRISPR/Cas-based multi-target DNA or RNA tests can be used for rapid and accurate diagnostic assays for plant viruses and viroids. Integrating NGS with CRISPR-based genome editing technologies may lead to a paradigm shift in combating deadly disease-causing plant viruses/viroids at the genomic level. Furthermore, the newly discovered CRISPR/Cas13 system has unprecedented potential in plant viroid diagnostics and interference. In this review, we have highlighted the application and importance of sequencing technologies on covering the viral genomes for precise modulations. This review also provides a snapshot vision of emerging developments in NGS technologies for the characterization of plant viruses and their potential utilities, advantages, and limitations in plant viral diagnostics. Furthermore, some of the notable advances like novel virus-inducible CRISPR/Cas9 system that confers virus resistance with no off-target effects have been discussed." @default.
- W3208390229 created "2021-11-08" @default.
- W3208390229 creator A5002622667 @default.
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- W3208390229 creator A5083005632 @default.
- W3208390229 creator A5084003470 @default.
- W3208390229 date "2021-10-25" @default.
- W3208390229 modified "2023-10-15" @default.
- W3208390229 title "Integrating CRISPR-Cas and Next Generation Sequencing in Plant Virology" @default.
- W3208390229 cites W10783906 @default.
- W3208390229 cites W1940949464 @default.
- W3208390229 cites W1964797774 @default.
- W3208390229 cites W1969522526 @default.
- W3208390229 cites W1974291581 @default.
- W3208390229 cites W1990379499 @default.
- W3208390229 cites W1992314272 @default.
- W3208390229 cites W1996390333 @default.
- W3208390229 cites W2001845502 @default.
- W3208390229 cites W2004622311 @default.
- W3208390229 cites W2007311563 @default.
- W3208390229 cites W2009521247 @default.
- W3208390229 cites W2018366017 @default.
- W3208390229 cites W2021767455 @default.
- W3208390229 cites W2030974598 @default.
- W3208390229 cites W2039375385 @default.
- W3208390229 cites W2041615659 @default.
- W3208390229 cites W2045435533 @default.
- W3208390229 cites W2047248338 @default.
- W3208390229 cites W2049455664 @default.
- W3208390229 cites W2063131041 @default.
- W3208390229 cites W2096261947 @default.
- W3208390229 cites W2100806677 @default.
- W3208390229 cites W2133342886 @default.
- W3208390229 cites W2135008913 @default.
- W3208390229 cites W2137450096 @default.
- W3208390229 cites W2139670358 @default.
- W3208390229 cites W2147419953 @default.
- W3208390229 cites W2149366757 @default.
- W3208390229 cites W2153344788 @default.
- W3208390229 cites W2154354934 @default.
- W3208390229 cites W2176976665 @default.
- W3208390229 cites W2234876210 @default.
- W3208390229 cites W2238012416 @default.
- W3208390229 cites W2257046203 @default.
- W3208390229 cites W2281379240 @default.
- W3208390229 cites W2328268714 @default.
- W3208390229 cites W2345123064 @default.
- W3208390229 cites W2398597893 @default.
- W3208390229 cites W2416796986 @default.
- W3208390229 cites W2530423224 @default.
- W3208390229 cites W2547631956 @default.
- W3208390229 cites W2576785575 @default.
- W3208390229 cites W2606915822 @default.
- W3208390229 cites W2607042046 @default.
- W3208390229 cites W2608659150 @default.
- W3208390229 cites W2608954324 @default.
- W3208390229 cites W2763042654 @default.
- W3208390229 cites W2763816339 @default.
- W3208390229 cites W2765284830 @default.
- W3208390229 cites W2765732718 @default.
- W3208390229 cites W2777524119 @default.
- W3208390229 cites W2783117772 @default.
- W3208390229 cites W2785904295 @default.
- W3208390229 cites W2787024379 @default.
- W3208390229 cites W2788036162 @default.
- W3208390229 cites W2793093904 @default.
- W3208390229 cites W2793359586 @default.
- W3208390229 cites W2795120137 @default.
- W3208390229 cites W2800905921 @default.
- W3208390229 cites W2801783434 @default.
- W3208390229 cites W2809533318 @default.
- W3208390229 cites W2847206774 @default.
- W3208390229 cites W2883233053 @default.
- W3208390229 cites W2886734910 @default.
- W3208390229 cites W2886825305 @default.
- W3208390229 cites W2888684269 @default.
- W3208390229 cites W2894759607 @default.
- W3208390229 cites W2895605803 @default.
- W3208390229 cites W2897700601 @default.
- W3208390229 cites W2903338689 @default.
- W3208390229 cites W2909108012 @default.
- W3208390229 cites W2909877469 @default.
- W3208390229 cites W2910181162 @default.
- W3208390229 cites W2912430338 @default.
- W3208390229 cites W2915823955 @default.
- W3208390229 cites W2917276353 @default.
- W3208390229 cites W2917293204 @default.
- W3208390229 cites W2918418208 @default.
- W3208390229 cites W2923285802 @default.
- W3208390229 cites W2944535522 @default.
- W3208390229 cites W2947089828 @default.
- W3208390229 cites W2951068216 @default.