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• W3208397947 abstract "Cytotoxic natural killer cells kill tumors and infected cells. We carried out CRISPR-based gene editing and transcriptional regulation in hard-to-manipulate NK-92 cells. NK-92-based therapies were found to be safe and efficacious in preclinical studies of cancers. Here, we have pioneered the generation and validation of NK-92 cells constitutively expressing Cas9 or dCas9 for knockout (CRISPRko), transcriptional activation (CRISPRa), or transcriptional repression (CRISPRi) of genes. Our CRISPR-engineered NK-92 cell platforms can be modified for research and off-the-shelf therapeutic applications." @default.
• W3208397947 created "2021-11-08" @default.
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• W3208397947 date "2021-12-01" @default.
• W3208397947 modified "2023-10-01" @default.
• W3208397947 title "Generation and validation of CRISPR-engineered human natural killer cell lines for research and therapeutic applications" @default.
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• W3208397947 doi "https://doi.org/10.1016/j.xpro.2021.100874" @default.
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