Matches in SemOpenAlex for { <https://semopenalex.org/work/W3208410154> ?p ?o ?g. }
- W3208410154 endingPage "1395" @default.
- W3208410154 startingPage "1395" @default.
- W3208410154 abstract "<ns4:p><ns4:bold>Background:</ns4:bold> Recent strides in computational structural biology have opened up an opportunity to understand previously uncharacterised proteins. The under-representation of transmembrane proteins in the Protein Data Bank highlights the need to apply new and advanced bioinformatics methods to shed light on their structure and function. This study focuses on a family of transmembrane proteins containing the Pfam domain PF09335 ('SNARE_ASSOC'/ ‘VTT ‘/’Tvp38’/'DedA'). One prominent member, Tmem41b, has been shown to be involved in early stages of autophagosome formation and is vital in mouse embryonic development as well as being identified as a viral host factor of SARS-CoV-2.</ns4:p><ns4:p> <ns4:bold>Methods:</ns4:bold> We used evolutionary covariance-derived information to construct and validate <ns4:italic>ab initio </ns4:italic>models, make domain boundary predictions and infer local structural features. </ns4:p><ns4:p> <ns4:bold>Results:</ns4:bold> The results from the structural bioinformatics analysis of Tmem41b and its homologues showed that they contain a tandem repeat that is clearly visible in evolutionary covariance data but much less so by sequence analysis. Furthermore, cross-referencing of other prediction data with covariance analysis showed that the internal repeat features two-fold rotational symmetry. <ns4:italic>Ab initio</ns4:italic> modelling of Tmem41b and homologues reinforces these structural predictions. Local structural features predicted to be present in Tmem41b were also present in Cl<ns4:sup>-</ns4:sup>/H<ns4:sup>+ </ns4:sup>antiporters. </ns4:p><ns4:p> <ns4:bold>Conclusions:</ns4:bold> The results of this study strongly point to Tmem41b and its homologues being transporters for an as-yet uncharacterised substrate and possibly using H<ns4:sup>+</ns4:sup> antiporter activity as its mechanism for transport.</ns4:p>" @default.
- W3208410154 created "2021-11-08" @default.
- W3208410154 creator A5009636964 @default.
- W3208410154 creator A5044560051 @default.
- W3208410154 creator A5060375119 @default.
- W3208410154 creator A5061400116 @default.
- W3208410154 creator A5091516295 @default.
- W3208410154 date "2021-03-25" @default.
- W3208410154 modified "2023-10-14" @default.
- W3208410154 title "In silico prediction of structure and function for a large family of transmembrane proteins that includes human Tmem41b" @default.
- W3208410154 cites W1173462427 @default.
- W3208410154 cites W1567897268 @default.
- W3208410154 cites W1863216998 @default.
- W3208410154 cites W1938173378 @default.
- W3208410154 cites W1970861588 @default.
- W3208410154 cites W1984680116 @default.
- W3208410154 cites W1995317616 @default.
- W3208410154 cites W2000610210 @default.
- W3208410154 cites W2008545402 @default.
- W3208410154 cites W2013863297 @default.
- W3208410154 cites W2033651986 @default.
- W3208410154 cites W2047567262 @default.
- W3208410154 cites W2050073991 @default.
- W3208410154 cites W2051210555 @default.
- W3208410154 cites W2051545676 @default.
- W3208410154 cites W2072470496 @default.
- W3208410154 cites W2083187008 @default.
- W3208410154 cites W2088433350 @default.
- W3208410154 cites W2105537627 @default.
- W3208410154 cites W2106119381 @default.
- W3208410154 cites W2106260605 @default.
- W3208410154 cites W2115170507 @default.
- W3208410154 cites W2119993590 @default.
- W3208410154 cites W2120772351 @default.
- W3208410154 cites W2137703950 @default.
- W3208410154 cites W2138872556 @default.
- W3208410154 cites W2140831051 @default.
- W3208410154 cites W2142529984 @default.
- W3208410154 cites W2145268834 @default.
- W3208410154 cites W2154139219 @default.
- W3208410154 cites W2275780625 @default.
- W3208410154 cites W2328111620 @default.
- W3208410154 cites W2342240481 @default.
- W3208410154 cites W2342569995 @default.
- W3208410154 cites W2376573086 @default.
- W3208410154 cites W2400386683 @default.
- W3208410154 cites W2426403057 @default.
- W3208410154 cites W2511608695 @default.
- W3208410154 cites W2524386004 @default.
- W3208410154 cites W2574496196 @default.
- W3208410154 cites W2601113975 @default.
- W3208410154 cites W2605518777 @default.
- W3208410154 cites W2774178591 @default.
- W3208410154 cites W2791655484 @default.
- W3208410154 cites W2885453664 @default.
- W3208410154 cites W2887384294 @default.
- W3208410154 cites W2888807543 @default.
- W3208410154 cites W2895814244 @default.
- W3208410154 cites W2898402099 @default.
- W3208410154 cites W2916586181 @default.
- W3208410154 cites W2923775987 @default.
- W3208410154 cites W2930774615 @default.
- W3208410154 cites W2970594786 @default.
- W3208410154 cites W2971733377 @default.
- W3208410154 cites W2986648423 @default.
- W3208410154 cites W2997234557 @default.
- W3208410154 cites W3092135414 @default.
- W3208410154 cites W3113360200 @default.
- W3208410154 cites W3121771115 @default.
- W3208410154 cites W4211079598 @default.
- W3208410154 doi "https://doi.org/10.12688/f1000research.27676.2" @default.
- W3208410154 hasPublicationYear "2021" @default.
- W3208410154 type Work @default.
- W3208410154 sameAs 3208410154 @default.
- W3208410154 citedByCount "6" @default.
- W3208410154 countsByYear W32084101542021 @default.
- W3208410154 countsByYear W32084101542023 @default.
- W3208410154 crossrefType "journal-article" @default.
- W3208410154 hasAuthorship W3208410154A5009636964 @default.
- W3208410154 hasAuthorship W3208410154A5044560051 @default.
- W3208410154 hasAuthorship W3208410154A5060375119 @default.
- W3208410154 hasAuthorship W3208410154A5061400116 @default.
- W3208410154 hasAuthorship W3208410154A5091516295 @default.
- W3208410154 hasBestOaLocation W32084101541 @default.
- W3208410154 hasConcept C86803240 @default.
- W3208410154 hasConceptScore W3208410154C86803240 @default.
- W3208410154 hasLocation W32084101541 @default.
- W3208410154 hasLocation W32084101542 @default.
- W3208410154 hasLocation W32084101543 @default.
- W3208410154 hasLocation W32084101544 @default.
- W3208410154 hasOpenAccess W3208410154 @default.
- W3208410154 hasPrimaryLocation W32084101541 @default.
- W3208410154 hasRelatedWork W1641042124 @default.
- W3208410154 hasRelatedWork W1990804418 @default.
- W3208410154 hasRelatedWork W1993764875 @default.
- W3208410154 hasRelatedWork W2013243191 @default.
- W3208410154 hasRelatedWork W2051339581 @default.
- W3208410154 hasRelatedWork W2082860237 @default.