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• W3208432013 endingPage "462643" @default.
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• W3208432013 abstract "Although inorganic silica-based and polymeric micron-sized spheres have widely been explored as column packing materials in high performance liquid chromatography (HPLC), they are still suffering the problems of either alkali corrosion of silica or polymer swelling. It is still necessary to search simple approaches for fabrication of monodisperse micron-sized hybrid particles as packing materials in HPLC. A novel kind of silicon-containing polyacrylate microspheres was designed and fabricated via two-step swelling and photo-initiated polymerization approach using 3-(allylpropylsilane) propyl acrylate (TAPA) containing both acrylate and vinyl groups and trimethylolpropane triacrylate (TRIM) as precursors. After carefully optimizing the fabrication conditions, the monodisperse micron-sized microspheres could be acquired as chromatographic packing, exhibiting excellent mechanical stability and reproducibility. Due to existence of electron-rich vinyl groups, three kinds of thiols such as octadecanethiol (ODT), dithiothreitol (DTT) and trimethylolpropane tris(3-mercaptopropionate) (TTMP) were facilely anchored onto the surface of microsphere via photo-initiated thiol-ene click reaction. They were applied in the separation of small molecules by cLC-UV and complex biosamples by cLC-MS/MS. A total of 6691 unique peptides from 1771 unique proteins was identified by ODT-modified microsphere, which was higher than those by unmodified and DTT/TTMP-modified poly(TAPA-co-TRIM) microspheres. It was expected this kind of hybrid microspheres can be further modified and widely applied in chromatographic field, offering great potential in commercialization." @default.
• W3208432013 created "2021-11-08" @default.
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• W3208432013 date "2021-12-01" @default.
• W3208432013 modified "2023-09-24" @default.
• W3208432013 title "Fabrication of highly crosslinked and monodispersed silicon-containing polymeric microspheres via photo-initiated polymerization and their application in capillary liquid chromatography" @default.
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• W3208432013 doi "https://doi.org/10.1016/j.chroma.2021.462643" @default.
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