Matches in SemOpenAlex for { <https://semopenalex.org/work/W32084904> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W32084904 abstract "Objective To investigate whether isolated blastomeres of discarded human embryos could develop into blastocysts cultured in vitro without zona pellucida. Methods Total discarded 60 embryos, which were not suitable for transplanting or freezing, were collected from 21 patients. Of 60 embryos, 10, 8, 24, 12, and 4 embryos were at 2-, 3-, 4-, 5- and 6-cell stage, respectively. These embryos were split by 0.5% protease combined with mechanical method. The resulting single blastomere was cultured individually, evaluated daily and counted blastocyst development. Pluripotency of inner cell mass (ICM) of the blastocyst was analyzed by the expression of alkaline phosphatase(AKP). Results A total of 229 single blastomeres were isolated from 60 embryos. Defining the day when the embryos were split as the first day (d 1). The majority of the blastomere-derived embryos followed the normal pattern of development with compaction on d 3 and cavitationon d 4 and developed into small blastocysts on d 5. Rates of division, compaction, cavity and expansion of single blastomeres from 4-cell embryos were higher than those in other groups (P Conclusion Some of the blastomeres from discarded human embryo are flexible and able to develop into blastocysts, the potential was related to their donor embryos closely. They seem to follow development pattern of their donor embryos. The blastomere-derived blastocysts have smaller size and fewer cells compared with regular in vitro cultured human embryos. However, AKP expression showed ICM cells with pluripotency. We believe that the value of single blastomere of discarded embryos will be further confirmed in the future." @default.
- W32084904 created "2016-06-24" @default.
- W32084904 creator A5014480953 @default.
- W32084904 creator A5021547417 @default.
- W32084904 creator A5031167388 @default.
- W32084904 creator A5039018137 @default.
- W32084904 creator A5047447008 @default.
- W32084904 creator A5070113542 @default.
- W32084904 date "2010-03-01" @default.
- W32084904 modified "2023-09-23" @default.
- W32084904 title "In-vitro Developmental Potential of Single Blastomeres Derived from Day 3 Discarded Human Embryos" @default.
- W32084904 cites W1934389460 @default.
- W32084904 cites W1966811171 @default.
- W32084904 cites W1989780518 @default.
- W32084904 cites W1989928096 @default.
- W32084904 cites W1993086095 @default.
- W32084904 cites W1993302506 @default.
- W32084904 cites W2016526297 @default.
- W32084904 cites W2030280138 @default.
- W32084904 cites W2032512845 @default.
- W32084904 cites W2038723730 @default.
- W32084904 cites W2101547758 @default.
- W32084904 cites W2114591772 @default.
- W32084904 cites W2123475048 @default.
- W32084904 cites W2147250856 @default.
- W32084904 cites W2153486820 @default.
- W32084904 cites W2285541383 @default.
- W32084904 cites W2460164359 @default.
- W32084904 doi "https://doi.org/10.1016/s1001-7844(10)60008-9" @default.
- W32084904 hasPublicationYear "2010" @default.
- W32084904 type Work @default.
- W32084904 sameAs 32084904 @default.
- W32084904 citedByCount "1" @default.
- W32084904 countsByYear W320849042020 @default.
- W32084904 crossrefType "journal-article" @default.
- W32084904 hasAuthorship W32084904A5014480953 @default.
- W32084904 hasAuthorship W32084904A5021547417 @default.
- W32084904 hasAuthorship W32084904A5031167388 @default.
- W32084904 hasAuthorship W32084904A5039018137 @default.
- W32084904 hasAuthorship W32084904A5047447008 @default.
- W32084904 hasAuthorship W32084904A5070113542 @default.
- W32084904 hasConcept C159399396 @default.
- W32084904 hasConcept C16685009 @default.
- W32084904 hasConcept C179933525 @default.
- W32084904 hasConcept C196843134 @default.
- W32084904 hasConcept C2776010925 @default.
- W32084904 hasConcept C2776690073 @default.
- W32084904 hasConcept C2778177303 @default.
- W32084904 hasConcept C68760520 @default.
- W32084904 hasConcept C71924100 @default.
- W32084904 hasConcept C86803240 @default.
- W32084904 hasConcept C87073359 @default.
- W32084904 hasConcept C95444343 @default.
- W32084904 hasConceptScore W32084904C159399396 @default.
- W32084904 hasConceptScore W32084904C16685009 @default.
- W32084904 hasConceptScore W32084904C179933525 @default.
- W32084904 hasConceptScore W32084904C196843134 @default.
- W32084904 hasConceptScore W32084904C2776010925 @default.
- W32084904 hasConceptScore W32084904C2776690073 @default.
- W32084904 hasConceptScore W32084904C2778177303 @default.
- W32084904 hasConceptScore W32084904C68760520 @default.
- W32084904 hasConceptScore W32084904C71924100 @default.
- W32084904 hasConceptScore W32084904C86803240 @default.
- W32084904 hasConceptScore W32084904C87073359 @default.
- W32084904 hasConceptScore W32084904C95444343 @default.
- W32084904 hasLocation W320849041 @default.
- W32084904 hasOpenAccess W32084904 @default.
- W32084904 hasPrimaryLocation W320849041 @default.
- W32084904 hasRelatedWork W1968365830 @default.
- W32084904 hasRelatedWork W1979252467 @default.
- W32084904 hasRelatedWork W1986446930 @default.
- W32084904 hasRelatedWork W2015666708 @default.
- W32084904 hasRelatedWork W2034574342 @default.
- W32084904 hasRelatedWork W2040114341 @default.
- W32084904 hasRelatedWork W2040309654 @default.
- W32084904 hasRelatedWork W2045324029 @default.
- W32084904 hasRelatedWork W2048983429 @default.
- W32084904 hasRelatedWork W2080411382 @default.
- W32084904 hasRelatedWork W2135693864 @default.
- W32084904 hasRelatedWork W2154016543 @default.
- W32084904 hasRelatedWork W2267091535 @default.
- W32084904 hasRelatedWork W2354231319 @default.
- W32084904 hasRelatedWork W2361269899 @default.
- W32084904 hasRelatedWork W2371805380 @default.
- W32084904 hasRelatedWork W2910974334 @default.
- W32084904 hasRelatedWork W3140634131 @default.
- W32084904 hasRelatedWork W3205259014 @default.
- W32084904 hasRelatedWork W2008148756 @default.
- W32084904 isParatext "false" @default.
- W32084904 isRetracted "false" @default.
- W32084904 magId "32084904" @default.
- W32084904 workType "article" @default.