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• W3208496628 abstract "We report a method of glycosylated enzymes' surface immobilisation and stabilisation. The enzyme is immobilised at the surface of silica nanoparticles through the reversible covalent binding of vicinal diols of the enzyme glycans with a surface-attached boronate derivative. A soft organosilica layer of controlled thickness is grown at the silica surface, entrapping the enzyme and thus avoiding enzyme leaching. We demonstrate that this approach results not only in high and durable activity retention but also enzyme stabilisation." @default.
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• W3208496628 date "2021-01-01" @default.
• W3208496628 modified "2023-10-03" @default.
• W3208496628 title "Immobilisation and stabilisation of glycosylated enzymes on boronic acid-functionalised silica nanoparticles" @default.
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• W3208496628 doi "https://doi.org/10.1039/d1cc04916j" @default.
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