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• W3208503162 abstract "Here, we review recent progress made on the genetic characterization of Giardia duodenalis assemblages and their relationship with virulence. We also discuss the implications of virulence factors in the pathogenesis of giardiasis, and advances in the development of vaccines and drugs based on knowledge of virulence markers. The use of transcriptomic and proteomic technologies as well as whole genome sequencing (WGS) from single cysts has allowed the assembly of the draft genome sequences for assemblages C and D of G. duodenalis. These findings, along with the published genomes for assemblages A, B, and E, have allowed comparative genomic investigations. In addition, the use of these methodologies for the characterization of the secretomes of trophozoite-epithelial cell interactions for assemblages A/B has led to the identification of virulence markers including energy metabolism enzymes, proteinases, high-cysteine membrane proteins (HCMPs), and variant surface proteins (VSPs). Recently, some drugs and vaccines, targeting virulence factors have been developed, offering possible alternatives to current treatment and prevention options against giardiasis. Among the nine recognized species of Giardia, G. duodenalis stands out because of its broad spectrum of hosts and its socio-economic importance. This species comprises eight genetic assemblages (A to H), of which A and B are zoonotic, and the other assemblages have narrow host specificities. Assemblages A and B may be considered as the most virulent ones, but the existence of asymptomatic carriers and considerable genetic variability within and among these assemblages hampers the definition of common virulence factors. The attachment of Giardia trophozoites to epithelial cells and structural cytoskeleton components of the adhesive disk, such as giardins or tubulins, is proposed to play key roles, but toxins have not yet been precisely defined. However, recent transcriptomic and proteomic analyses of the secretomes of trophozoites representing assemblages A and B and interacting with particular epithelial cell lines have defined a series of virulence factors, including glycolytic (e.g., enolase) and arginolytic (e.g., arginine deiminase) enzymes, cysteine proteases (e.g., giardipain-1) and VSPs (e.g., VSP9B10A). Other factors, such as HCMPs and tenascins, have been consistently found to be excreted/secreted, but their role(s) in the pathogenesis of giardiasis has not yet been elucidated. Interestingly, recent investigations of single cysts representing assemblages C and D using advanced sequencing and informatic methods have suggested that the transcription/expression profiles of virulence factors vary both within and between assemblages, thus assemblage-specific molecules might allow adaptation to the microenvironment within the host. Importantly, some drugs active against cysteine-rich proteins of Giardia, including giardipain-1, VSPs and arginine deiminase, have been shown to be targeted by cysteine-modifying compounds as disulfiram, L-canavanin and allicin. On the other hand, VSPs are presently considered as key vaccine candidates because they induce protection against Giardia in rodents and dogs. Overall, this review reveals that much more work is needed to identify, characterize, and understand the roles of virulence factors in Giardia and to assess their validity as drug and vaccine targets. Clear, advanced omics and informatic tools should assist in this future endeavor, with a focus on targeting virulence factors that are common and/or unique to distinct assemblages to develop new and effective interventions against Giardia." @default.
• W3208503162 created "2021-11-08" @default.
• W3208503162 creator A5000662999 @default.
• W3208503162 creator A5025752446 @default.
• W3208503162 date "2021-10-21" @default.
• W3208503162 modified "2023-10-16" @default.
• W3208503162 title "Giardia duodenalis Virulence — “To Be, or Not To Be”" @default.
• W3208503162 cites W1490286156 @default.
• W3208503162 cites W1564662492 @default.
• W3208503162 cites W1831118780 @default.
• W3208503162 cites W1925668163 @default.
• W3208503162 cites W1968307525 @default.
• W3208503162 cites W1970083470 @default.
• W3208503162 cites W1972825953 @default.
• W3208503162 cites W1975336675 @default.
• W3208503162 cites W1980403905 @default.
• W3208503162 cites W1981846308 @default.
• W3208503162 cites W1984687624 @default.
• W3208503162 cites W1986208999 @default.
• W3208503162 cites W1988227922 @default.
• W3208503162 cites W2007985461 @default.
• W3208503162 cites W2019858741 @default.
• W3208503162 cites W2020451552 @default.
• W3208503162 cites W2020612126 @default.
• W3208503162 cites W2028677604 @default.
• W3208503162 cites W2030684171 @default.
• W3208503162 cites W2031888903 @default.
• W3208503162 cites W2043428681 @default.
• W3208503162 cites W2043871099 @default.
• W3208503162 cites W2050472019 @default.
• W3208503162 cites W2052152201 @default.
• W3208503162 cites W2064960064 @default.
• W3208503162 cites W2072778963 @default.
• W3208503162 cites W2072861322 @default.
• W3208503162 cites W2073178782 @default.
• W3208503162 cites W2075683777 @default.
• W3208503162 cites W2078727354 @default.
• W3208503162 cites W2082120087 @default.
• W3208503162 cites W2087514066 @default.
• W3208503162 cites W2092339922 @default.
• W3208503162 cites W2097046679 @default.
• W3208503162 cites W2101451490 @default.
• W3208503162 cites W2106125311 @default.
• W3208503162 cites W2115438056 @default.
• W3208503162 cites W2116994121 @default.
• W3208503162 cites W2117159489 @default.
• W3208503162 cites W2117628638 @default.
• W3208503162 cites W2139425464 @default.
• W3208503162 cites W2141346104 @default.
• W3208503162 cites W2141688115 @default.
• W3208503162 cites W2147043577 @default.
• W3208503162 cites W2152238692 @default.
• W3208503162 cites W2154607802 @default.
• W3208503162 cites W2158144866 @default.
• W3208503162 cites W2159049823 @default.
• W3208503162 cites W2160121979 @default.
• W3208503162 cites W2165921856 @default.
• W3208503162 cites W2166003238 @default.
• W3208503162 cites W2167958993 @default.
• W3208503162 cites W2168676619 @default.
• W3208503162 cites W2177561135 @default.
• W3208503162 cites W2184405267 @default.
• W3208503162 cites W2254181201 @default.
• W3208503162 cites W2267775177 @default.
• W3208503162 cites W2517798081 @default.
• W3208503162 cites W2519199842 @default.
• W3208503162 cites W2564180726 @default.
• W3208503162 cites W2566592218 @default.
• W3208503162 cites W2590003621 @default.
• W3208503162 cites W2601341455 @default.
• W3208503162 cites W2606004738 @default.
• W3208503162 cites W2732482688 @default.
• W3208503162 cites W2735013611 @default.
• W3208503162 cites W2745866105 @default.
• W3208503162 cites W2775171339 @default.
• W3208503162 cites W2775793257 @default.
• W3208503162 cites W2782570775 @default.
• W3208503162 cites W2787773054 @default.
• W3208503162 cites W2790284643 @default.
• W3208503162 cites W2800486018 @default.
• W3208503162 cites W2804929930 @default.
• W3208503162 cites W2805054868 @default.
• W3208503162 cites W2811306079 @default.
• W3208503162 cites W2883986102 @default.
• W3208503162 cites W2897025776 @default.
• W3208503162 cites W2900816440 @default.
• W3208503162 cites W2921841164 @default.
• W3208503162 cites W2953564680 @default.
• W3208503162 cites W2980515281 @default.
• W3208503162 cites W2984605916 @default.
• W3208503162 cites W2996488076 @default.
• W3208503162 cites W2999742500 @default.
• W3208503162 cites W3064872615 @default.
• W3208503162 cites W3093418501 @default.
• W3208503162 doi "https://doi.org/10.1007/s40475-021-00248-z" @default.
• W3208503162 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8529366" @default.
• W3208503162 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34697581" @default.
• W3208503162 hasPublicationYear "2021" @default.

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