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- W3208544035 abstract "As a model of neurodegenerative disease, the study on SH-SY5Y cells is of great significance due to the complexity of cause and difficulty in cure. Based on this reality, we studied the influence of culture duration on the morphology and mechanical properties of living SH-SY5Y cells by atomic force microscopy (AFM). A comparative analysis was made to characterize the cell size and tip-cell adhesion, and their increased trends with the increase of cell culture duration were obtained, providing a guideline for the physiological characteristics of the cells. As the culture time increased from 24[Formula: see text]h to 48[Formula: see text]h, the measured average height and width of cells were increased by 0.59[Formula: see text][Formula: see text]m and 1.38[Formula: see text][Formula: see text]m, respectively. As for cell elasticity, statistical analysis showed that the difference between different parts of the cell was obvious. Additionally, the electrophysiological characteristic as an important indicator for measuring the functional activities of SH-SY5Y cells was investigated. Here, a homemade conductive atomic force microscope (C-AFM) provided an effective way for membrane potential recordings. It was successfully used to study the real-time responses of living SH-SY5Y cells to mechanical insertions, and a membrane potential with an amplitude of [Formula: see text][Formula: see text]V was obtained without losing cell viability." @default.
- W3208544035 created "2021-11-08" @default.
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- W3208544035 date "2021-10-21" @default.
- W3208544035 modified "2023-10-17" @default.
- W3208544035 title "Mechanical Properties and Electrical Behavior of Neuroblastoma Cells Characterized by Atomic Force Microscopy" @default.
- W3208544035 doi "https://doi.org/10.1142/s179329202150140x" @default.
- W3208544035 hasPublicationYear "2021" @default.
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