FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3208579898> ?p ?o ?g. }

• W3208579898 endingPage "18720" @default.
• W3208579898 startingPage "18714" @default.
• W3208579898 abstract "Spatiotemporally resolved dissection of subcellular proteome is crucial to our understanding of cellular functions in health and disease. We herein report a bioorthogonal and photocatalytic decaging-enabled proximity labeling strategy (CAT-Prox) for spatiotemporally resolved mitochondrial proteome profiling in living cells. Our systematic survey of the photocatalysts has led to the identification of Ir(ppy)2bpy as a bioorthogonal and mitochondria-targeting catalyst that allowed photocontrolled, rapid rescue of azidobenzyl-caged quinone methide as a highly reactive Michael acceptor for proximity-based protein labeling in mitochondria of live cells. Upon careful validation through in vitro labeling, mitochondria-targeting specificity, in situ catalytic activity as well as protein tagging, we applied CAT-Prox for mitochondria proteome profiling in living Hela cells as well as hard-to-transfect macrophage RAW264.7 cells with approximately 70% mitochondria specificity observed from up to 300 proteins enriched. Finally, CAT-Prox was further applied to the dynamic dissection of mitochondria proteome of macrophage cells upon lipopolysaccharide stimulation. By integrating photocatalytic decaging chemistry with proximity-based protein labeling, CAT-Prox offers a general, catalytic, and nongenetic alternative to the enzyme-based proximity labeling strategies for diverse live cell settings." @default.
• W3208579898 created "2021-11-08" @default.
• W3208579898 creator A5003266319 @default.
• W3208579898 creator A5024128036 @default.
• W3208579898 creator A5032373349 @default.
• W3208579898 creator A5045364623 @default.
• W3208579898 creator A5078032929 @default.
• W3208579898 creator A5078325885 @default.
• W3208579898 creator A5081811268 @default.
• W3208579898 creator A5090314772 @default.
• W3208579898 date "2021-10-28" @default.
• W3208579898 modified "2023-10-16" @default.
• W3208579898 title "Bioorthogonal Photocatalytic Decaging-Enabled Mitochondrial Proteomics" @default.
• W3208579898 cites W1967143550 @default.
• W3208579898 cites W1977039417 @default.
• W3208579898 cites W1980496079 @default.
• W3208579898 cites W1996941025 @default.
• W3208579898 cites W1999818082 @default.
• W3208579898 cites W2031084334 @default.
• W3208579898 cites W2072362345 @default.
• W3208579898 cites W2085952412 @default.
• W3208579898 cites W2093361250 @default.
• W3208579898 cites W2131415852 @default.
• W3208579898 cites W2141656162 @default.
• W3208579898 cites W2283105825 @default.
• W3208579898 cites W2407177587 @default.
• W3208579898 cites W2460752692 @default.
• W3208579898 cites W2606546819 @default.
• W3208579898 cites W2613629789 @default.
• W3208579898 cites W2738647095 @default.
• W3208579898 cites W2888618670 @default.
• W3208579898 cites W2901173163 @default.
• W3208579898 cites W2913566944 @default.
• W3208579898 cites W2928665623 @default.
• W3208579898 cites W2982058149 @default.
• W3208579898 cites W3010320439 @default.
• W3208579898 cites W3042902202 @default.
• W3208579898 cites W3084705282 @default.
• W3208579898 cites W3093564279 @default.
• W3208579898 cites W3121727740 @default.
• W3208579898 cites W3158193076 @default.
• W3208579898 cites W3160066326 @default.
• W3208579898 cites W3175902314 @default.
• W3208579898 cites W3180578233 @default.
• W3208579898 cites W4211185070 @default.
• W3208579898 doi "https://doi.org/10.1021/jacs.1c09171" @default.
• W3208579898 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34709827" @default.
• W3208579898 hasPublicationYear "2021" @default.
• W3208579898 type Work @default.
• W3208579898 sameAs 3208579898 @default.
• W3208579898 citedByCount "29" @default.
• W3208579898 countsByYear W32085798982022 @default.
• W3208579898 countsByYear W32085798982023 @default.
• W3208579898 crossrefType "journal-article" @default.
• W3208579898 hasAuthorship W3208579898A5003266319 @default.
• W3208579898 hasAuthorship W3208579898A5024128036 @default.
• W3208579898 hasAuthorship W3208579898A5032373349 @default.
• W3208579898 hasAuthorship W3208579898A5045364623 @default.
• W3208579898 hasAuthorship W3208579898A5078032929 @default.
• W3208579898 hasAuthorship W3208579898A5078325885 @default.
• W3208579898 hasAuthorship W3208579898A5081811268 @default.
• W3208579898 hasAuthorship W3208579898A5090314772 @default.
• W3208579898 hasConcept C104317684 @default.
• W3208579898 hasConcept C104397665 @default.
• W3208579898 hasConcept C185592680 @default.
• W3208579898 hasConcept C21951064 @default.
• W3208579898 hasConcept C28859421 @default.
• W3208579898 hasConcept C46111723 @default.
• W3208579898 hasConcept C55493867 @default.
• W3208579898 hasConcept C69610077 @default.
• W3208579898 hasConcept C86803240 @default.
• W3208579898 hasConcept C930959 @default.
• W3208579898 hasConcept C95444343 @default.
• W3208579898 hasConceptScore W3208579898C104317684 @default.
• W3208579898 hasConceptScore W3208579898C104397665 @default.
• W3208579898 hasConceptScore W3208579898C185592680 @default.
• W3208579898 hasConceptScore W3208579898C21951064 @default.
• W3208579898 hasConceptScore W3208579898C28859421 @default.
• W3208579898 hasConceptScore W3208579898C46111723 @default.
• W3208579898 hasConceptScore W3208579898C55493867 @default.
• W3208579898 hasConceptScore W3208579898C69610077 @default.
• W3208579898 hasConceptScore W3208579898C86803240 @default.
• W3208579898 hasConceptScore W3208579898C930959 @default.
• W3208579898 hasConceptScore W3208579898C95444343 @default.
• W3208579898 hasFunder F4320321001 @default.
• W3208579898 hasFunder F4320321540 @default.
• W3208579898 hasFunder F4320322919 @default.
• W3208579898 hasIssue "44" @default.
• W3208579898 hasLocation W32085798981 @default.
• W3208579898 hasLocation W32085798982 @default.
• W3208579898 hasOpenAccess W3208579898 @default.
• W3208579898 hasPrimaryLocation W32085798981 @default.
• W3208579898 hasRelatedWork W1982829397 @default.
• W3208579898 hasRelatedWork W1999412725 @default.
• W3208579898 hasRelatedWork W2086860678 @default.
• W3208579898 hasRelatedWork W2106414650 @default.
• W3208579898 hasRelatedWork W2114732768 @default.
• W3208579898 hasRelatedWork W2124137418 @default.

• next