Matches in SemOpenAlex for { <https://semopenalex.org/work/W3208666929> ?p ?o ?g. }
- W3208666929 abstract "The neurovascular unit (NVU), consisting of neurons, glial cells, vascular cells (endothelial cells, pericytes and vascular smooth muscle cells (VSMCs)) together with the surrounding extracellular matrix (ECM), is an important interface between the peripheral blood and the brain parenchyma. Disruption of the NVU impacts on blood-brain barrier (BBB) regulation and underlies the development and pathology of multiple neurological disorders, including stroke and Alzheimer's disease (AD). The ability to differentiate induced pluripotent stem cells (iPSCs) into the different cell types of the NVU and incorporate them into physical models provides a reverse engineering approach to generate human NVU models to study BBB function. To recapitulate the in vivo situation such NVU models must also incorporate the ECM to provide a 3D environment with appropriate mechanical and biochemical cues for the cells of the NVU. In this review, we provide an overview of the cells of the NVU and the surrounding ECM, before discussing the characteristics (stiffness, functionality and porosity) required of hydrogels to mimic the ECM when incorporated into in vitro NVU models. We summarise the approaches available to measure BBB functionality and present the techniques in use to develop robust and translatable models of the NVU, including transwell models, hydrogel models, 3D-bioprinting, microfluidic models and organoids. The incorporation of iPSCs either without or with disease-specific genetic mutations into these NVU models provides a platform in which to study normal and disease mechanisms, test BBB permeability to drugs, screen for new therapeutic targets and drugs or to design cell-based therapies." @default.
- W3208666929 created "2021-11-08" @default.
- W3208666929 creator A5021063040 @default.
- W3208666929 creator A5051029209 @default.
- W3208666929 creator A5069996663 @default.
- W3208666929 date "2021-11-09" @default.
- W3208666929 modified "2023-10-11" @default.
- W3208666929 title "3D hydrogel models of the neurovascular unit to investigate blood–brain barrier dysfunction" @default.
- W3208666929 cites W1526691126 @default.
- W3208666929 cites W1563180177 @default.
- W3208666929 cites W1584771014 @default.
- W3208666929 cites W1667567520 @default.
- W3208666929 cites W1843099843 @default.
- W3208666929 cites W1855166775 @default.
- W3208666929 cites W1862832045 @default.
- W3208666929 cites W1866745427 @default.
- W3208666929 cites W1913314522 @default.
- W3208666929 cites W1947402635 @default.
- W3208666929 cites W1964354605 @default.
- W3208666929 cites W1965896403 @default.
- W3208666929 cites W1967722867 @default.
- W3208666929 cites W1971163936 @default.
- W3208666929 cites W1971500643 @default.
- W3208666929 cites W1973772667 @default.
- W3208666929 cites W1976837011 @default.
- W3208666929 cites W1977784832 @default.
- W3208666929 cites W1980036375 @default.
- W3208666929 cites W1982367836 @default.
- W3208666929 cites W1982397697 @default.
- W3208666929 cites W1985140092 @default.
- W3208666929 cites W1985914247 @default.
- W3208666929 cites W1988200792 @default.
- W3208666929 cites W1992758695 @default.
- W3208666929 cites W1996964516 @default.
- W3208666929 cites W1997218140 @default.
- W3208666929 cites W2001726213 @default.
- W3208666929 cites W2002665694 @default.
- W3208666929 cites W2006123434 @default.
- W3208666929 cites W2009364907 @default.
- W3208666929 cites W2010415067 @default.
- W3208666929 cites W2011290724 @default.
- W3208666929 cites W2014932510 @default.
- W3208666929 cites W2020061739 @default.
- W3208666929 cites W2022106720 @default.
- W3208666929 cites W2026053645 @default.
- W3208666929 cites W2026208845 @default.
- W3208666929 cites W2026332221 @default.
- W3208666929 cites W2027098220 @default.
- W3208666929 cites W2028231726 @default.
- W3208666929 cites W2029378715 @default.
- W3208666929 cites W2035471044 @default.
- W3208666929 cites W2035516992 @default.
- W3208666929 cites W2042261220 @default.
- W3208666929 cites W2042706188 @default.
- W3208666929 cites W2045242267 @default.
- W3208666929 cites W2045899778 @default.
- W3208666929 cites W2051140555 @default.
- W3208666929 cites W2052664641 @default.
- W3208666929 cites W2056229778 @default.
- W3208666929 cites W2057164916 @default.
- W3208666929 cites W2060358364 @default.
- W3208666929 cites W2065245517 @default.
- W3208666929 cites W2067733328 @default.
- W3208666929 cites W2067935721 @default.
- W3208666929 cites W2072524978 @default.
- W3208666929 cites W2072975935 @default.
- W3208666929 cites W2073880678 @default.
- W3208666929 cites W2074661552 @default.
- W3208666929 cites W2074705887 @default.
- W3208666929 cites W2076503648 @default.
- W3208666929 cites W2076634690 @default.
- W3208666929 cites W2077240652 @default.
- W3208666929 cites W2077673959 @default.
- W3208666929 cites W2079907174 @default.
- W3208666929 cites W2081135553 @default.
- W3208666929 cites W2081510823 @default.
- W3208666929 cites W2082079718 @default.
- W3208666929 cites W2082314612 @default.
- W3208666929 cites W2082844918 @default.
- W3208666929 cites W2087356761 @default.
- W3208666929 cites W2093417927 @default.
- W3208666929 cites W2099361891 @default.
- W3208666929 cites W2104591424 @default.
- W3208666929 cites W2111256015 @default.
- W3208666929 cites W2113887671 @default.
- W3208666929 cites W2114118213 @default.
- W3208666929 cites W2114622532 @default.
- W3208666929 cites W2117481374 @default.
- W3208666929 cites W2117691576 @default.
- W3208666929 cites W2118191386 @default.
- W3208666929 cites W2120471129 @default.
- W3208666929 cites W2126231259 @default.
- W3208666929 cites W2129553803 @default.
- W3208666929 cites W2129605132 @default.
- W3208666929 cites W2130639482 @default.
- W3208666929 cites W2134208433 @default.
- W3208666929 cites W2135401759 @default.
- W3208666929 cites W2135789763 @default.
- W3208666929 cites W2139123746 @default.
- W3208666929 cites W2144734692 @default.