Matches in SemOpenAlex for { <https://semopenalex.org/work/W3208697730> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W3208697730 abstract "Background: Epitope mapping is an increasingly important aspect of biotherapeutic and vaccine development. Recent advances in therapeutic antibody design and production has enabled candidate mAbs to be identified at a rapidly increasing rate resulting in a significant bottleneck in the characterization of ‘structural’ epitopes, that are challenging to determine using existing high throughput epitope mapping tools. Here, Hydrogen/Deuterium Exchange Mass Spectrometry (HDX-MS) epitope screening workflow was introduced that is well suited for accelerated characterization of epitopes with a common antigen. Main methods and major results: The method is demonstrated on set of 6 candidate mAbs targeting Pertactin (PRN). Using this approach, five of the six epitopes was unambiguously determined using two HDX mixing timepoints in 24 hours total run time, corresponding to substantial decrease in the instrument time required to map a single epitope using conventional HDX workflows. Conclusion: An accelerated HDX-MS epitope screening workflow was developed. The two-timepoint ‘screening’ workflow mapped all six mAbs and generated high confidence epitopes for five of the six mAbs assayed. The substantial improvement in the rate of data collection can advance HDX-MS for higher throughput investigations supporting the ability to evaluate a broader number of mAb candidates at an earlier stage of vaccine development." @default.
- W3208697730 created "2021-11-08" @default.
- W3208697730 creator A5010232366 @default.
- W3208697730 creator A5040829410 @default.
- W3208697730 creator A5051602996 @default.
- W3208697730 creator A5079336524 @default.
- W3208697730 creator A5087332105 @default.
- W3208697730 creator A5091087100 @default.
- W3208697730 date "2021-07-27" @default.
- W3208697730 modified "2023-09-27" @default.
- W3208697730 title "Epitope Screening Using Hydrogen/Deuterium Exchange Mass Spectrometry (HDX-MS): An Optimized Workflow for Accelerated Evaluation of Lead Monoclonal Antibodies" @default.
- W3208697730 doi "https://doi.org/10.22541/au.162742356.68704724/v1" @default.
- W3208697730 hasPublicationYear "2021" @default.
- W3208697730 type Work @default.
- W3208697730 sameAs 3208697730 @default.
- W3208697730 citedByCount "0" @default.
- W3208697730 crossrefType "posted-content" @default.
- W3208697730 hasAuthorship W3208697730A5010232366 @default.
- W3208697730 hasAuthorship W3208697730A5040829410 @default.
- W3208697730 hasAuthorship W3208697730A5051602996 @default.
- W3208697730 hasAuthorship W3208697730A5079336524 @default.
- W3208697730 hasAuthorship W3208697730A5087332105 @default.
- W3208697730 hasAuthorship W3208697730A5091087100 @default.
- W3208697730 hasBestOaLocation W32086977301 @default.
- W3208697730 hasConcept C147483822 @default.
- W3208697730 hasConcept C159654299 @default.
- W3208697730 hasConcept C162356407 @default.
- W3208697730 hasConcept C167569220 @default.
- W3208697730 hasConcept C177212765 @default.
- W3208697730 hasConcept C185592680 @default.
- W3208697730 hasConcept C195616568 @default.
- W3208697730 hasConcept C203014093 @default.
- W3208697730 hasConcept C2781241564 @default.
- W3208697730 hasConcept C41008148 @default.
- W3208697730 hasConcept C43617362 @default.
- W3208697730 hasConcept C542903549 @default.
- W3208697730 hasConcept C70721500 @default.
- W3208697730 hasConcept C77088390 @default.
- W3208697730 hasConcept C86803240 @default.
- W3208697730 hasConceptScore W3208697730C147483822 @default.
- W3208697730 hasConceptScore W3208697730C159654299 @default.
- W3208697730 hasConceptScore W3208697730C162356407 @default.
- W3208697730 hasConceptScore W3208697730C167569220 @default.
- W3208697730 hasConceptScore W3208697730C177212765 @default.
- W3208697730 hasConceptScore W3208697730C185592680 @default.
- W3208697730 hasConceptScore W3208697730C195616568 @default.
- W3208697730 hasConceptScore W3208697730C203014093 @default.
- W3208697730 hasConceptScore W3208697730C2781241564 @default.
- W3208697730 hasConceptScore W3208697730C41008148 @default.
- W3208697730 hasConceptScore W3208697730C43617362 @default.
- W3208697730 hasConceptScore W3208697730C542903549 @default.
- W3208697730 hasConceptScore W3208697730C70721500 @default.
- W3208697730 hasConceptScore W3208697730C77088390 @default.
- W3208697730 hasConceptScore W3208697730C86803240 @default.
- W3208697730 hasLocation W32086977301 @default.
- W3208697730 hasOpenAccess W3208697730 @default.
- W3208697730 hasPrimaryLocation W32086977301 @default.
- W3208697730 hasRelatedWork W1604248381 @default.
- W3208697730 hasRelatedWork W1971169755 @default.
- W3208697730 hasRelatedWork W1999614011 @default.
- W3208697730 hasRelatedWork W2046065180 @default.
- W3208697730 hasRelatedWork W2063604066 @default.
- W3208697730 hasRelatedWork W2124915684 @default.
- W3208697730 hasRelatedWork W2162389703 @default.
- W3208697730 hasRelatedWork W2400620861 @default.
- W3208697730 hasRelatedWork W3044660350 @default.
- W3208697730 hasRelatedWork W3105097885 @default.
- W3208697730 isParatext "false" @default.
- W3208697730 isRetracted "false" @default.
- W3208697730 magId "3208697730" @default.
- W3208697730 workType "article" @default.