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• W3208943257 abstract "Phytophthoraroot and stem rot (PRSR), caused by oomycete Phytophthora sojae, is themost severe soil-borne disease of soybean (Glycine max (L.) Merr.) worldwide.The disease can be effectively managed by introducing resistance to P. sojae(Rps) genes into soybean cultivars by breeding, which requirescontinuous efforts on identification of resistance resources from soybeangermplasm. Previously, two resistance genes, Rps2-cas (former name Rps2-das)and Rps14 (former name Rps1-f), were mapped by linkage analysisfrom soybean landraces, PI 594549 C and PI 340029, respectively. The resistanceunderlying PI 594592 also need further characterization given its broadresistance spectrum. In this study, Rps-2cas and Rps14 werefurther mapped, and Rps2-b, was identified and initial mapped from PI594592. Thus, this thesis research was divided into three parts for three Rpsgenes.The first partmainly focuses advances on Rps2-cas. Marker-assisted spectrum analysiswas performed for Rps-2cas to confirm its potential in diseasemanagement. A high-quality genome assembly of PI 594549 C was generated, andKASP markers were developed based on comparison between new reference andWilliams 82 reference genome. The gene was further mapped to a 32.67-kb regionon PI 594549 C reference genome harboring three expressed NLRs by 24recombinants screened from a large F4 population. Comparativegenomics analysis suggests the only intact NBS-LRR gene in the fine mappingregion is the best candidate gene for Rps2cas, and its function wasvalidated by stable transformation. Evidences from other high-quality assemblygenomes suggest Rps2-cas originated from an ancient unequal crossingover event.In the secondpart, Rps14 was further mapped using 21 recombinants identified from a F3population consisting of 473 plants. In commonly used Williams 82reference genome, the assembly of fine mapping region was incomplete, and Rps14region showed drastic variation in size and copy number of NLRs in 23high-quality genome assemblies, suggesting the complexity of Rps14region and high-quality reference sequence of donor line is required forisolation of Rps14 candidate genes. Marker assisted resistance testshowed Rps14 had wider resistance spectrum to different P. sojae isolatescomparing to other Rps genes on chromosome 3, and phylogenic analysisfurther supported the potential of Rps14 to be a novel resistance gene. For the thirdpart, an F2 population derived from a cross between PI 594592 andWilliams was tested by P. sojae race 1. The 3:1 and 1:2:1 Mendeliansegregation ratios were observed in F2 individuals and F2:3 families,respectively, suggesting a single dominant Rps gene in PI 594592. Thegene was initially mapped to the distal end chromosome 16 overlapped with Rps2,and the gene was tentatively named as Rps2-b. Polymorphic SSR markersand InDel markers designed based on re-sequencing data of PI 594592 andWilliams was used to genotyping all the F2:3 families, and a linkagemap was constructed for Rps2-b. Rps2-b was mapped to a 461.8-kbregion flanked by SSR marker Satt431 and InDel marker InDel3668 according to thereference genome (Wm82. a2). Marker-assisted resistance test showed Rps2-bhold a wide resistance spectrum." @default.
• W3208943257 created "2021-11-08" @default.
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• W3208943257 date "2021-07-29" @default.
• W3208943257 modified "2023-09-23" @default.
• W3208943257 title "Molecular identification of Phytophthora resistant genes in soybean" @default.
• W3208943257 doi "https://doi.org/10.25394/pgs.15074508.v1" @default.
• W3208943257 hasPublicationYear "2021" @default.
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