Matches in SemOpenAlex for { <https://semopenalex.org/work/W3208985324> ?p ?o ?g. }
- W3208985324 endingPage "100030" @default.
- W3208985324 startingPage "100030" @default.
- W3208985324 abstract "The study of electrical activity in single cells and local circuits of excitable cells, such as neurons, requires an easy-to-use, high-throughput methodology that allows for the measurement of membrane potential. Investigating the electrical properties in specific subcompartments of neurons, or in a specific type of neurons, introduces additional complexity. An optical voltage-imaging technique that allows high spatial and temporal resolution could be an ideal solution. However, most valid voltage-imaging techniques are nonspecific. Those that are more site-directed require a lot of preliminary work and specific adaptations, among other drawbacks. Here, we explore a new method for membrane voltage imaging, based on Förster resonance energy transfer between fluorescent polystyrene (FPS) beads and dipicrylamine. Not only has it been shown that fluorescence intensity correlates with membrane potential, but more importantly, the membrane potential from individual particles can be detected. Among other advantages, FPS beads can be synthesized with surface functional groups and can be targeted to specific proteins by conjugation of recognition molecules. Therefore, in the presence of dipicrylamine, FPS beads represent single-particle detectors of membrane potential that can be localized to specific membrane compartments. This new and easily accessible platform for targeted optical voltage imaging can further elucidate the mechanisms of neuronal electrical activity." @default.
- W3208985324 created "2021-11-08" @default.
- W3208985324 creator A5010821195 @default.
- W3208985324 creator A5040695867 @default.
- W3208985324 creator A5058017365 @default.
- W3208985324 creator A5060401840 @default.
- W3208985324 creator A5064370530 @default.
- W3208985324 date "2021-12-01" @default.
- W3208985324 modified "2023-10-14" @default.
- W3208985324 title "Optical probing of local membrane potential with fluorescent polystyrene beads" @default.
- W3208985324 cites W1992866186 @default.
- W3208985324 cites W2000206662 @default.
- W3208985324 cites W2004318321 @default.
- W3208985324 cites W2007733019 @default.
- W3208985324 cites W2009667219 @default.
- W3208985324 cites W2015199368 @default.
- W3208985324 cites W2032735451 @default.
- W3208985324 cites W2040785083 @default.
- W3208985324 cites W2050786281 @default.
- W3208985324 cites W2057349955 @default.
- W3208985324 cites W2059825619 @default.
- W3208985324 cites W2061744336 @default.
- W3208985324 cites W2063575064 @default.
- W3208985324 cites W2065550726 @default.
- W3208985324 cites W2072770548 @default.
- W3208985324 cites W2088047695 @default.
- W3208985324 cites W2090568616 @default.
- W3208985324 cites W2111778626 @default.
- W3208985324 cites W2139980848 @default.
- W3208985324 cites W2141194930 @default.
- W3208985324 cites W2257974883 @default.
- W3208985324 cites W2403944530 @default.
- W3208985324 cites W2525787459 @default.
- W3208985324 cites W2560111916 @default.
- W3208985324 cites W2736996438 @default.
- W3208985324 cites W2908861291 @default.
- W3208985324 cites W2909328871 @default.
- W3208985324 cites W2943665347 @default.
- W3208985324 cites W2979747856 @default.
- W3208985324 cites W2995091889 @default.
- W3208985324 cites W3159593375 @default.
- W3208985324 doi "https://doi.org/10.1016/j.bpr.2021.100030" @default.
- W3208985324 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34939044" @default.
- W3208985324 hasPublicationYear "2021" @default.
- W3208985324 type Work @default.
- W3208985324 sameAs 3208985324 @default.
- W3208985324 citedByCount "1" @default.
- W3208985324 countsByYear W32089853242021 @default.
- W3208985324 crossrefType "journal-article" @default.
- W3208985324 hasAuthorship W3208985324A5010821195 @default.
- W3208985324 hasAuthorship W3208985324A5040695867 @default.
- W3208985324 hasAuthorship W3208985324A5058017365 @default.
- W3208985324 hasAuthorship W3208985324A5060401840 @default.
- W3208985324 hasAuthorship W3208985324A5064370530 @default.
- W3208985324 hasBestOaLocation W32089853241 @default.
- W3208985324 hasConcept C120665830 @default.
- W3208985324 hasConcept C121332964 @default.
- W3208985324 hasConcept C12554922 @default.
- W3208985324 hasConcept C171250308 @default.
- W3208985324 hasConcept C181911157 @default.
- W3208985324 hasConcept C185592680 @default.
- W3208985324 hasConcept C186060115 @default.
- W3208985324 hasConcept C192562407 @default.
- W3208985324 hasConcept C41625074 @default.
- W3208985324 hasConcept C55493867 @default.
- W3208985324 hasConcept C57477423 @default.
- W3208985324 hasConcept C86803240 @default.
- W3208985324 hasConcept C91881484 @default.
- W3208985324 hasConcept C96305047 @default.
- W3208985324 hasConceptScore W3208985324C120665830 @default.
- W3208985324 hasConceptScore W3208985324C121332964 @default.
- W3208985324 hasConceptScore W3208985324C12554922 @default.
- W3208985324 hasConceptScore W3208985324C171250308 @default.
- W3208985324 hasConceptScore W3208985324C181911157 @default.
- W3208985324 hasConceptScore W3208985324C185592680 @default.
- W3208985324 hasConceptScore W3208985324C186060115 @default.
- W3208985324 hasConceptScore W3208985324C192562407 @default.
- W3208985324 hasConceptScore W3208985324C41625074 @default.
- W3208985324 hasConceptScore W3208985324C55493867 @default.
- W3208985324 hasConceptScore W3208985324C57477423 @default.
- W3208985324 hasConceptScore W3208985324C86803240 @default.
- W3208985324 hasConceptScore W3208985324C91881484 @default.
- W3208985324 hasConceptScore W3208985324C96305047 @default.
- W3208985324 hasFunder F4320306076 @default.
- W3208985324 hasFunder F4320306084 @default.
- W3208985324 hasFunder F4320320338 @default.
- W3208985324 hasFunder F4320322252 @default.
- W3208985324 hasFunder F4320338335 @default.
- W3208985324 hasIssue "2" @default.
- W3208985324 hasLocation W32089853241 @default.
- W3208985324 hasLocation W32089853242 @default.
- W3208985324 hasLocation W32089853243 @default.
- W3208985324 hasLocation W32089853244 @default.
- W3208985324 hasLocation W32089853245 @default.
- W3208985324 hasLocation W32089853246 @default.
- W3208985324 hasLocation W32089853247 @default.
- W3208985324 hasLocation W32089853248 @default.
- W3208985324 hasOpenAccess W3208985324 @default.