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• W3209170437 abstract "Copper-sulfhydryl complexes in wine can be disrupted by addition of brine to release free hydrogen sulfide (H2S), and the resulting brine-releasable H2S is reported to correlate with formation of H2S during bottle storage. However, both the mechanism of the brine-release assay and factors affecting the stability of copper sulfhydryls under brine-release conditions are not well understood. By varying brine composition and concentration, it is shown that release of copper-complexed H2S requires the presence of a halide (Cl- and Br-) and is not due to a general salting-out effect. Release of copper-complexed H2S by the brine dilution assay is highly temperature-dependent. When H2S and Cu(II) are added to a model wine, brine-releasable H2S decreases markedly (∼10-fold) after a 20 min incubation period prior to performing the brine-release assay. In commercial wines, the fraction of added H2S recovered through the brine-release assay was correlated with the initial glutathione (GSH) concentration (r2 = 0.58) but not with initial Cu. Negligible additional release of H2S from organopolysulfanes was observed following addition of a disulfide-reducing agent (tris(2-carboxyethyl)phosphine). As previous studies have reported a correlation between H2S formed under brine-release conditions and normal storage, these results suggest that the susceptibility of a wine to form latent copper-sulfhydryl precursors of H2S following copper addition is dependent on the concentration of sulfhydryls like GSH." @default.
• W3209170437 created "2021-11-08" @default.
• W3209170437 creator A5006796442 @default.
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• W3209170437 date "2021-10-28" @default.
• W3209170437 modified "2023-09-26" @default.
• W3209170437 title "Brine-Releasable Hydrogen Sulfide in Wine: Mechanism of Release from Copper Complexes and Effects of Glutathione" @default.
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• W3209170437 doi "https://doi.org/10.1021/acs.jafc.1c04885" @default.
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