Matches in SemOpenAlex for { <https://semopenalex.org/work/W3209238838> ?p ?o ?g. }
- W3209238838 abstract "Abstract Clusters of ryanodine receptor calcium channels (RyRs) form the primary molecular machinery in cardiomyocytes. Various adaptations of super-resolution microscopy have revealed intricate details of the structure, molecular composition and locations of these couplons. However, most optical super-resolution techniques lack the capacity for three-dimensional (3D) visualisation. Enhanced Expansion Microscopy (EExM) offers resolution (in-plane and axially) sufficient to spatially resolve individual proteins within peripheral couplons and within dyads located in the interior. We have combined immunocytochemistry and immunohistochemistry variations of EExM with 3D visualisation to examine the complex topologies, geometries and molecular sub-domains within RyR clusters. We observed that peripheral couplons exhibit variable co-clustering ratios and patterns between RyR and the structural protein, junctophilin-2 (JPH2). Dyads possessed sub-domains of JPH2 which occupied the central regions of the RyR cluster, whilst the poles were typically devoid of JPH2 and broader, and likely specialise in turnover and remodelling of the cluster. In right ventricular myocytes from rats with monocrotaline-induced right ventricular failure, we observed hallmarks of RyR cluster fragmentation accompanied by similar fragmentations of the JPH2 sub-domains. We hypothesise that the frayed morphology of RyRs in close proximity to fragmented JPH2 structural sub-domains may form the primordial foci of RyR mobilisation and dyad remodelling." @default.
- W3209238838 created "2021-11-08" @default.
- W3209238838 creator A5014123456 @default.
- W3209238838 creator A5014298019 @default.
- W3209238838 creator A5016335570 @default.
- W3209238838 creator A5049939552 @default.
- W3209238838 creator A5068412492 @default.
- W3209238838 creator A5072741787 @default.
- W3209238838 date "2021-10-22" @default.
- W3209238838 modified "2023-09-27" @default.
- W3209238838 title "Molecular-scale 3D visualisation of the cardiac ryanodine receptor clusters and the molecular-scale fraying of dyads" @default.
- W3209238838 cites W1964683921 @default.
- W3209238838 cites W1986683817 @default.
- W3209238838 cites W1994304532 @default.
- W3209238838 cites W1995628893 @default.
- W3209238838 cites W2022142440 @default.
- W3209238838 cites W2030267203 @default.
- W3209238838 cites W2046526051 @default.
- W3209238838 cites W2046895212 @default.
- W3209238838 cites W2049849698 @default.
- W3209238838 cites W2050824263 @default.
- W3209238838 cites W2054225000 @default.
- W3209238838 cites W2065076724 @default.
- W3209238838 cites W2072670197 @default.
- W3209238838 cites W2090116945 @default.
- W3209238838 cites W2116529502 @default.
- W3209238838 cites W2127041759 @default.
- W3209238838 cites W2130264694 @default.
- W3209238838 cites W2142344615 @default.
- W3209238838 cites W2145582089 @default.
- W3209238838 cites W2162408579 @default.
- W3209238838 cites W2460281481 @default.
- W3209238838 cites W2533359311 @default.
- W3209238838 cites W2744858607 @default.
- W3209238838 cites W2784007059 @default.
- W3209238838 cites W2785004435 @default.
- W3209238838 cites W2804777919 @default.
- W3209238838 cites W2897886929 @default.
- W3209238838 cites W2898269107 @default.
- W3209238838 cites W2899411827 @default.
- W3209238838 cites W2904180818 @default.
- W3209238838 cites W2906540700 @default.
- W3209238838 cites W2917151158 @default.
- W3209238838 cites W2999699470 @default.
- W3209238838 cites W3011892935 @default.
- W3209238838 cites W3030236448 @default.
- W3209238838 cites W3040121687 @default.
- W3209238838 cites W3193545391 @default.
- W3209238838 cites W4210456529 @default.
- W3209238838 doi "https://doi.org/10.1101/2021.10.21.462365" @default.
- W3209238838 hasPublicationYear "2021" @default.
- W3209238838 type Work @default.
- W3209238838 sameAs 3209238838 @default.
- W3209238838 citedByCount "3" @default.
- W3209238838 countsByYear W32092388382022 @default.
- W3209238838 countsByYear W32092388382023 @default.
- W3209238838 crossrefType "posted-content" @default.
- W3209238838 hasAuthorship W3209238838A5014123456 @default.
- W3209238838 hasAuthorship W3209238838A5014298019 @default.
- W3209238838 hasAuthorship W3209238838A5016335570 @default.
- W3209238838 hasAuthorship W3209238838A5049939552 @default.
- W3209238838 hasAuthorship W3209238838A5068412492 @default.
- W3209238838 hasAuthorship W3209238838A5072741787 @default.
- W3209238838 hasBestOaLocation W32092388381 @default.
- W3209238838 hasConcept C105702510 @default.
- W3209238838 hasConcept C113217602 @default.
- W3209238838 hasConcept C12554922 @default.
- W3209238838 hasConcept C158617107 @default.
- W3209238838 hasConcept C164866538 @default.
- W3209238838 hasConcept C185592680 @default.
- W3209238838 hasConcept C199360897 @default.
- W3209238838 hasConcept C41008148 @default.
- W3209238838 hasConcept C86803240 @default.
- W3209238838 hasConcept C95444343 @default.
- W3209238838 hasConceptScore W3209238838C105702510 @default.
- W3209238838 hasConceptScore W3209238838C113217602 @default.
- W3209238838 hasConceptScore W3209238838C12554922 @default.
- W3209238838 hasConceptScore W3209238838C158617107 @default.
- W3209238838 hasConceptScore W3209238838C164866538 @default.
- W3209238838 hasConceptScore W3209238838C185592680 @default.
- W3209238838 hasConceptScore W3209238838C199360897 @default.
- W3209238838 hasConceptScore W3209238838C41008148 @default.
- W3209238838 hasConceptScore W3209238838C86803240 @default.
- W3209238838 hasConceptScore W3209238838C95444343 @default.
- W3209238838 hasLocation W32092388381 @default.
- W3209238838 hasOpenAccess W3209238838 @default.
- W3209238838 hasPrimaryLocation W32092388381 @default.
- W3209238838 hasRelatedWork W1969707292 @default.
- W3209238838 hasRelatedWork W2003800797 @default.
- W3209238838 hasRelatedWork W2040299448 @default.
- W3209238838 hasRelatedWork W2046475516 @default.
- W3209238838 hasRelatedWork W2110538544 @default.
- W3209238838 hasRelatedWork W2144742768 @default.
- W3209238838 hasRelatedWork W2150611110 @default.
- W3209238838 hasRelatedWork W2585328935 @default.
- W3209238838 hasRelatedWork W2951542374 @default.
- W3209238838 hasRelatedWork W3128955856 @default.
- W3209238838 isParatext "false" @default.
- W3209238838 isRetracted "false" @default.
- W3209238838 magId "3209238838" @default.