Matches in SemOpenAlex for { <https://semopenalex.org/work/W3209328851> ?p ?o ?g. }
- W3209328851 endingPage "13428" @default.
- W3209328851 startingPage "13415" @default.
- W3209328851 abstract "Single-molecule fluorescence imaging is among the most advanced analytical technologies and has been widely adopted for biosensing due to its distinct advantages of simplicity, rapidity, high sensitivity, low sample consumption, and visualization capability. Recently, a variety of nucleic acid amplification approaches have been developed to provide a straightforward and highly efficient way for amplifying low abundance target signals. The integration of single-molecule fluorescence imaging with nucleic acid amplification has greatly facilitated the construction of various fluorescent biosensors for in vitro and in vivo detection of DNAs, RNAs, enzymes, and live cells with high sensitivity and good selectivity. Herein, we review the advances in the development of fluorescent biosensors by integrating single-molecule fluorescence imaging with nucleic acid amplification based on enzyme (e.g., DNA polymerase, RNA polymerase, exonuclease, and endonuclease)-assisted and enzyme-free (e.g., catalytic hairpin assembly, entropy-driven DNA amplification, ligation chain reaction, and hybridization chain reaction) strategies, and summarize the principles, features, and in vitro and in vivo applications of the emerging biosensors. Moreover, we discuss the remaining challenges and future directions in this area. This review may inspire the development of new signal-amplified single-molecule biosensors and promote their practical applications in fundamental and clinical research." @default.
- W3209328851 created "2021-11-08" @default.
- W3209328851 creator A5001568746 @default.
- W3209328851 creator A5053220969 @default.
- W3209328851 creator A5079912108 @default.
- W3209328851 date "2021-01-01" @default.
- W3209328851 modified "2023-10-12" @default.
- W3209328851 title "Nucleic acid amplification-integrated single-molecule fluorescence imaging for <i>in vitro</i> and <i>in vivo</i> biosensing" @default.
- W3209328851 cites W1520883967 @default.
- W3209328851 cites W1544923801 @default.
- W3209328851 cites W1831033366 @default.
- W3209328851 cites W1901733664 @default.
- W3209328851 cites W1974527699 @default.
- W3209328851 cites W1976149758 @default.
- W3209328851 cites W1976260744 @default.
- W3209328851 cites W1977926175 @default.
- W3209328851 cites W1991453437 @default.
- W3209328851 cites W1992818586 @default.
- W3209328851 cites W1993859324 @default.
- W3209328851 cites W2004864721 @default.
- W3209328851 cites W2012040132 @default.
- W3209328851 cites W2014468482 @default.
- W3209328851 cites W2017660057 @default.
- W3209328851 cites W2020366763 @default.
- W3209328851 cites W2020451212 @default.
- W3209328851 cites W2022483739 @default.
- W3209328851 cites W2023041614 @default.
- W3209328851 cites W2027001273 @default.
- W3209328851 cites W2029230493 @default.
- W3209328851 cites W2032118018 @default.
- W3209328851 cites W2034630077 @default.
- W3209328851 cites W2035494017 @default.
- W3209328851 cites W2035792726 @default.
- W3209328851 cites W2037902796 @default.
- W3209328851 cites W2040206084 @default.
- W3209328851 cites W2044432713 @default.
- W3209328851 cites W2046387526 @default.
- W3209328851 cites W2046498107 @default.
- W3209328851 cites W2047236144 @default.
- W3209328851 cites W2050387685 @default.
- W3209328851 cites W2050630660 @default.
- W3209328851 cites W2054486764 @default.
- W3209328851 cites W2055222641 @default.
- W3209328851 cites W2061981862 @default.
- W3209328851 cites W2062083667 @default.
- W3209328851 cites W2066081299 @default.
- W3209328851 cites W2067332276 @default.
- W3209328851 cites W2068047533 @default.
- W3209328851 cites W2075487746 @default.
- W3209328851 cites W2090462692 @default.
- W3209328851 cites W2091068879 @default.
- W3209328851 cites W2091414779 @default.
- W3209328851 cites W2091602444 @default.
- W3209328851 cites W2093404711 @default.
- W3209328851 cites W2094437628 @default.
- W3209328851 cites W2100538295 @default.
- W3209328851 cites W2102699655 @default.
- W3209328851 cites W2105275554 @default.
- W3209328851 cites W2109263179 @default.
- W3209328851 cites W2115770874 @default.
- W3209328851 cites W2118022632 @default.
- W3209328851 cites W2121236750 @default.
- W3209328851 cites W2122139577 @default.
- W3209328851 cites W2125569948 @default.
- W3209328851 cites W2138032051 @default.
- W3209328851 cites W2142539585 @default.
- W3209328851 cites W2145303234 @default.
- W3209328851 cites W2146573461 @default.
- W3209328851 cites W2150695488 @default.
- W3209328851 cites W2151366879 @default.
- W3209328851 cites W2152656267 @default.
- W3209328851 cites W2160505835 @default.
- W3209328851 cites W2161863487 @default.
- W3209328851 cites W2169756563 @default.
- W3209328851 cites W2172461241 @default.
- W3209328851 cites W2198153867 @default.
- W3209328851 cites W2259212906 @default.
- W3209328851 cites W2313356495 @default.
- W3209328851 cites W2321092928 @default.
- W3209328851 cites W2379742646 @default.
- W3209328851 cites W2417125981 @default.
- W3209328851 cites W2517162516 @default.
- W3209328851 cites W2573750366 @default.
- W3209328851 cites W2588088434 @default.
- W3209328851 cites W2594225908 @default.
- W3209328851 cites W2613196727 @default.
- W3209328851 cites W2624547014 @default.
- W3209328851 cites W2625437134 @default.
- W3209328851 cites W2739705061 @default.
- W3209328851 cites W2753219571 @default.
- W3209328851 cites W2755909955 @default.
- W3209328851 cites W2771719355 @default.
- W3209328851 cites W2783842036 @default.
- W3209328851 cites W2783855936 @default.
- W3209328851 cites W2785861340 @default.
- W3209328851 cites W2790046171 @default.
- W3209328851 cites W2806353183 @default.
- W3209328851 cites W2888333468 @default.