FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3209333921> ?p ?o ?g. }

• W3209333921 abstract "Cardiac contractility modulation (CCM) is an intracardiac therapy whereby nonexcitatory electrical simulations are delivered during the absolute refractory period of the cardiac cycle. We previously evaluated the effects of CCM in isolated adult rabbit ventricular cardiomyocytes and found a transient increase in calcium and contractility. In the present study, we sought to extend these results to human cardiomyocytes using human induced pluripotent stem cell–derived cardiomyocytes (hiPSC-CMs) to develop a robust model to evaluate CCM in vitro. HiPSC-CMs (iCell Cardiomyocytes2, Fujifilm Cellular Dynamic, Inc.) were studied in monolayer format plated on flexible substrate. Contractility, calcium handling, and electrophysiology were evaluated by fluorescence- and video-based analysis (CellOPTIQ, Clyde Biosciences). CCM pulses were applied using an A-M Systems 4100 pulse generator. Robust hiPSC-CMs response was observed at 14 V/cm (64 mA) for pacing and 28 V/cm (128 mA, phase amplitude) for CCM. Under these conditions, hiPSC-CMs displayed enhanced contractile properties including increased contraction amplitude and faster contraction kinetics. Likewise, calcium transient amplitude increased, and calcium kinetics were faster. Furthermore, electrophysiological properties were altered resulting in shortened action potential duration (APD). The observed effects subsided when the CCM stimulation was stopped. CCM-induced increase in hiPSC-CMs contractility was significantly more pronounced when extracellular calcium concentration was lowered from 2 mM to 0.5 mM. This study provides a comprehensive characterization of CCM effects on hiPSC-CMs. These data represent the first study of CCM in hiPSC-CMs and provide an in vitro model to assess physiologically relevant mechanisms and evaluate safety and effectiveness of future cardiac electrophysiology medical devices." @default.
• W3209333921 created "2021-11-08" @default.
• W3209333921 creator A5000402589 @default.
• W3209333921 creator A5028177602 @default.
• W3209333921 creator A5037564625 @default.
• W3209333921 creator A5078177663 @default.
• W3209333921 date "2021-11-01" @default.
• W3209333921 modified "2023-10-06" @default.
• W3209333921 title "Acute effects of cardiac contractility modulation on human induced pluripotent stem cell–derived cardiomyocytes" @default.
• W3209333921 cites W1730627877 @default.
• W3209333921 cites W1876131152 @default.
• W3209333921 cites W1969832018 @default.
• W3209333921 cites W1996138247 @default.
• W3209333921 cites W2017071823 @default.
• W3209333921 cites W2025600249 @default.
• W3209333921 cites W2040003420 @default.
• W3209333921 cites W2050824263 @default.
• W3209333921 cites W2065572778 @default.
• W3209333921 cites W2068175148 @default.
• W3209333921 cites W2088461131 @default.
• W3209333921 cites W2104745579 @default.
• W3209333921 cites W2116011790 @default.
• W3209333921 cites W2123778449 @default.
• W3209333921 cites W2124929662 @default.
• W3209333921 cites W2129207505 @default.
• W3209333921 cites W2134897733 @default.
• W3209333921 cites W2154395421 @default.
• W3209333921 cites W2164577396 @default.
• W3209333921 cites W2256912117 @default.
• W3209333921 cites W2318641702 @default.
• W3209333921 cites W2406043282 @default.
• W3209333921 cites W2475024815 @default.
• W3209333921 cites W2527750418 @default.
• W3209333921 cites W2556601106 @default.
• W3209333921 cites W2592882315 @default.
• W3209333921 cites W2607906581 @default.
• W3209333921 cites W2610084826 @default.
• W3209333921 cites W2775448139 @default.
• W3209333921 cites W2804024542 @default.
• W3209333921 cites W2876121811 @default.
• W3209333921 cites W2893420171 @default.
• W3209333921 cites W2902201411 @default.
• W3209333921 cites W2964172723 @default.
• W3209333921 cites W2996381282 @default.
• W3209333921 cites W3027461818 @default.
• W3209333921 cites W3073642655 @default.
• W3209333921 cites W3091998877 @default.
• W3209333921 doi "https://doi.org/10.14814/phy2.15085" @default.
• W3209333921 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8564440" @default.
• W3209333921 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34729935" @default.
• W3209333921 hasPublicationYear "2021" @default.
• W3209333921 type Work @default.
• W3209333921 sameAs 3209333921 @default.
• W3209333921 citedByCount "10" @default.
• W3209333921 countsByYear W32093339212022 @default.
• W3209333921 countsByYear W32093339212023 @default.
• W3209333921 crossrefType "journal-article" @default.
• W3209333921 hasAuthorship W3209333921A5000402589 @default.
• W3209333921 hasAuthorship W3209333921A5028177602 @default.
• W3209333921 hasAuthorship W3209333921A5037564625 @default.
• W3209333921 hasAuthorship W3209333921A5078177663 @default.
• W3209333921 hasBestOaLocation W32093339213 @default.
• W3209333921 hasConcept C12554922 @default.
• W3209333921 hasConcept C126322002 @default.
• W3209333921 hasConcept C163415756 @default.
• W3209333921 hasConcept C164705383 @default.
• W3209333921 hasConcept C181080969 @default.
• W3209333921 hasConcept C185263204 @default.
• W3209333921 hasConcept C185592680 @default.
• W3209333921 hasConcept C189327125 @default.
• W3209333921 hasConcept C39133596 @default.
• W3209333921 hasConcept C519063684 @default.
• W3209333921 hasConcept C71924100 @default.
• W3209333921 hasConcept C80093799 @default.
• W3209333921 hasConcept C86803240 @default.
• W3209333921 hasConceptScore W3209333921C12554922 @default.
• W3209333921 hasConceptScore W3209333921C126322002 @default.
• W3209333921 hasConceptScore W3209333921C163415756 @default.
• W3209333921 hasConceptScore W3209333921C164705383 @default.
• W3209333921 hasConceptScore W3209333921C181080969 @default.
• W3209333921 hasConceptScore W3209333921C185263204 @default.
• W3209333921 hasConceptScore W3209333921C185592680 @default.
• W3209333921 hasConceptScore W3209333921C189327125 @default.
• W3209333921 hasConceptScore W3209333921C39133596 @default.
• W3209333921 hasConceptScore W3209333921C519063684 @default.
• W3209333921 hasConceptScore W3209333921C71924100 @default.
• W3209333921 hasConceptScore W3209333921C80093799 @default.
• W3209333921 hasConceptScore W3209333921C86803240 @default.
• W3209333921 hasIssue "21" @default.
• W3209333921 hasLocation W32093339211 @default.
• W3209333921 hasLocation W32093339212 @default.
• W3209333921 hasLocation W32093339213 @default.
• W3209333921 hasLocation W32093339214 @default.
• W3209333921 hasLocation W32093339215 @default.
• W3209333921 hasOpenAccess W3209333921 @default.
• W3209333921 hasPrimaryLocation W32093339211 @default.
• W3209333921 hasRelatedWork W2014732872 @default.
• W3209333921 hasRelatedWork W2053247927 @default.
• W3209333921 hasRelatedWork W2123765920 @default.
• W3209333921 hasRelatedWork W2359060722 @default.

• next