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• W3209693417 abstract "Single-cell analysis (SCA) is an important technology used in the fields of cell biology and biomedicine. The birth and development of SCA is based on modern advanced optical technology. So far, a large variety of optical detection methods have been used for SCA, including optical tweezing, super-resolution optical microscopy, dynamic light scattering, fluorescence imaging, evanescent-wave sensing, and hyperspectral microscopy. This chapter focuses on the recent applications of the latter two methods in SCA. Optical evanescent-wave sensors applied for SCA mainly include surface plasmon resonance (SPR) imaging sensors, plasmon waveguide resonance (PWR) imaging sensors, and metal-free waveguide resonance (WGR) sensors, all of which are label-free and noninvasive. Because optical evanescent wave is nonradiated surface electromagnetic wave, the earlier-mentioned sensors are highly sensitive to changes of refractive index induced by chemical and biological reactions on the surface. Nowadays, SPR and PWR imaging sensors and grating-coupled WGR sensor have been well recognized as label-free biosensing platforms for temporally and spatially resolved detection of intercellular and intracellular interactions, cell–substrate interaction, cell–drug molecule interaction, and cell apoptosis process. Hyperspectral microscopy is an informative multifunctional analytical technique based on a combination of the advanced hyperspectral imaging and the conventional optical microscopy such as dark-field microscopy and fluorescence microscopy. Hyperspectral microscopy with high resolution has been used for recognition and detection of metal nanostructures inside single cells, and it can provide spatially resolved quantitative information of intracellular compositional variation, giving deep insights on intracellular reaction. The typical experimental results selected from the relevant papers published so far are shown in this chapter to help readers to understand the roles of the aforementioned optical techniques for SCA. The pros and cons of each technique and the perspectives of further developments are discussed in conclusions." @default.
• W3209693417 created "2021-11-08" @default.
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• W3209693417 date "2022-01-01" @default.
• W3209693417 modified "2023-09-25" @default.
• W3209693417 title "Single-cell analysis by evanescent wave sensing and hyperspectral microscopy" @default.
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• W3209693417 doi "https://doi.org/10.1016/b978-0-323-89841-6.00003-7" @default.
• W3209693417 hasPublicationYear "2022" @default.
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